Cytochrome oxidase subunit 1 and mitochondrial 16S rDNA sequences of Trichuris skrjabini (Tricocephalida: Trichuridae)

Callejón, Rocío; Rojas, Manuel de; Ariza, C.; Úbeda, J. M.; Guevara, Diego; Cutillas, Cristina

doi:10.1007/s00436-008-1280-1

Cited by 13 publications

(6 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, COI gene amplified all the samples thus demonstrating that COI is a better gene for this purpose. The effectiveness of COI gene in species classification has also been reported before in wildlife [9], nematodes [32], reedbuck [10], birds [33], rodents [15], and lepidoptera [34]. The COI genes have been the most frequent methods used for species identification in animal biological studies due to its high degree of phylogenetic species differentiation as compared to other mtDNA genes [33].…”

Section: Discussionmentioning

confidence: 64%

Identification of Rodent Species That Infest Poultry Houses in Mafikeng, North West Province, South Africa

Ramatla

Mphuthi

Gofaone

et al. 2019

International Journal of Zoology

View full text Add to dashboard Cite

Rodents cause serious adverse effects on farm production due to destruction of food, contamination of feed, and circulation of diseases. The extent of damage or the diseases spread will depend on the type of rodents that invade the farm. This study was conducted in order to find out the species of rodents that infest poultry farms around Mafikeng, North West Province of South Africa. The study was part of a broader project that was investigating Salmonella vectors in the poultry farms around the province. The study trapped 154 rodents from selected farms and used the Cytochrome oxidase subunit 1 (COI) and the Cytochrome b (Cyt-b) barcoding genes for species identification. Two rodent pest species, namely, Rattus rattus (the black rat) and Rattus tanezumi (the Asian Rat/Asian House Rat) were identified. A total of 99 (64.3%) were identified as Rattus rattus and 55 (35.7%) were Rattus tanezumi. Between the two target genes, Cyt-b gene was only able to identify 40 (25.97%) of the total samples while COI was more efficient and amplified all the samples and thus was a better target gene for this kind of identification. The two rat species identified are known vectors of serious diseases; thus their presence should be regarded as an indication of high risk for diseases. Despite having been detected in the country before, finding R. tanezumi as the second largest rat species in the area was unexpected since this species is known to be indigenous to Asia.

show abstract

Section: Discussionmentioning

confidence: 64%

Identification of Rodent Species That Infest Poultry Houses in Mafikeng, North West Province, South Africa

Ramatla

Mphuthi

Gofaone

et al. 2019

International Journal of Zoology

View full text Add to dashboard Cite

show abstract

“…found in ruminants (both domesticated and non-domesticated). Four Trichuris spp.-T. discolor, T. ovis, T. globulosa and T. skrjabini-have been identified as inhabiting the caecum and colon of ruminants [35][36][37][38][39][40][41][42][43][44][45]. One of the major discoveries was the identification of T. globulosa and T. ovis as the same species by isoenzymes [35], using second, internally transcribed spacer ribosomal DNA (ITS2 rDNA) [38] and ITS1-5.8S-1TS2 [37].…”

Section: Molecular Identification Of Trichuris Spp In Domestic and Non-domestic Ruminantsmentioning

confidence: 99%

Trichuris spp. in Animals, with Specific Reference to Neo-Tropical Rodents

Jones

2021

Veterinary Sciences

View full text Add to dashboard Cite

Trichuriasis is the clinical disease of animals infected with the parasite of the genus Trichuris. This review attempts to present information on Trichuris spp. infestation in neo-tropical rodents that are utilized for meat consumption by humans. Neo-tropical rodents utilized for meat production can be divided into two categories: those that have been domesticated, which include the guinea pig (Cavia porcellus), and those that are on the verge of domestication, such as the capybara (Hydrochoerus hydrochaeris), lappe (Cuniculus paca/Agouti paca), and agouti (Dasyprocta leporina). This document reviews the literature on the species of Trichuris that affects the rodents mentioned above, as well as the clinical signs observed. The literature obtained spans over sixty years, from 1951 to 2020. Trichuris spp. was found in these neo-tropical rodents mentioned. However, there is a dearth of information on the species of Trichuris that parasitize these animals. The capybara was the only rodent where some molecular techniques were used to identify a new species named T. cutillasae. In most cases, Trichuris spp. was found in combination with other endoparasites, and was found at a low prevalence in the lappe and guinea pig. The presence of Trichuris spp. ranged from 4.62–53.85% in the agouti, 4.21–10.00% in the lappe, 50% in the capybaras, and 1–31% in guinea pigs. Further work must be done towards molecular identification of various Trichuris spp. present in these rodents, as well as the clinical effect of infection on the performance of agouti, lappe, capybara, and guinea pigs.

show abstract

“…PCR was performed using the following conditions: an initial denaturation at 96 °C for 3 min and then 30 cycles of 1 min at 94 °C, 1 min at 58 °C and 1 min at 72 °C, followed by 10 min at 72 °C. Finally, a 419 bp fragment of the cox1 gene was amplified using the forward primer L6625 (5'-TTYTGRTTYTTYGGNCAYCC-3') and reverse primer H7005 (5'-ACTACGTAGTAGGTATCATG-3'), which were together with PCR conditions modified by Callejón et al (2009). Each PCR mixture included: 12.5 μl of commercial Combi PPP Master Mix (Top-Bio, Praha, Czech Republic), 9.5 μl of PCR water, 1 μl of each primer mix (0.01 mM each) and 1 μl of template DNA.…”

Section: Pcr Amplification and Sequencingmentioning

confidence: 99%

How many species of whipworms do we share? Whipworms from man and other primates form two phylogenetic lineages

et al. 2015

View full text Add to dashboard Cite

Abstract:The whipworms, i.e. parasitic nematodes of the genus Trichuris Roederer, 1761, infect a variety of mammals. Apparently low diversity of primate-infecting species of Trichuris strongly contrasts with the high number of species described in other mammalian hosts. The present study addresses the diversity of whipworms in captive and free-ranging primates and humans by analysing nuclear (18S rRNA, ITS2) and mitochondrial (cox1) DNA. Phylogenetic analyses revealed that primate whipworms form two independent lineages: (i) the Trichuris trichiura (Linnaeus, 1771) clade comprised of genetically almost identical whipworms from human and other primates, which suggests the ability of T. trichiura to infect a broader range of primates; (ii) a clade containing primarily Trichuris suis Schrank, 1788, where isolates from human and various primates formed a sister group to isolates from pigs; the former isolates thus may represent of more species of Trichuris in primates including humans. The analysis of cox1 has shown the polyphyly of the genera Trichuris and Capillaria, Zeder, 1800. High sequence similarity of the T. trichiura isolates from humans and other primates suggests their zoonotic potential, although the extent of transmission between human and other non-human primates remains questionable and requires further study.

show abstract

Cytochrome oxidase subunit 1 and mitochondrial 16S rDNA sequences of Trichuris skrjabini (Tricocephalida: Trichuridae)

Cited by 13 publications

References 14 publications

Identification of Rodent Species That Infest Poultry Houses in Mafikeng, North West Province, South Africa

Identification of Rodent Species That Infest Poultry Houses in Mafikeng, North West Province, South Africa

Trichuris spp. in Animals, with Specific Reference to Neo-Tropical Rodents

How many species of whipworms do we share? Whipworms from man and other primates form two phylogenetic lineages

Contact Info

Product

Resources

About