Cytochrome d induction in Escherichia coli growing under unfavorable conditions

Abstract

Growth of .E. coli in the presence of the protonophorous uncoupler pentachlorophenol is shown to strongly enhance levels of cytochrome d, a putative Na+-motive oxidase. This effect was found to be arrested by chloramphenicol and stimulated by high Na+ concentration in the growth medium. The induction of cytochrome d takes place in a mutant deficient in the FoF, ATP-synthase but does not occur in mutants deficient in either of two different components of the Arc system. Similar relationships were revealed when pentachlorophenol was replaced by ferricyanide and phenazine methosulfate, agents oxidizing the respiratory chain. Induction of cytochrome dis also shown to occur in riboflavin-deficient mutants growing in the presence of such low riboflavin concentrations as to be insutlicient to maintain a high respiration rate. It is suggested (i) that it is &+ decrease rather than reduction of the respiratory chain that is the signal for the induction of cytochrome d, and (ii) the Arc system is involved in this type of metabolic regulation.

The Global Regulator ArcA Controls Resistance to Reactive Nitrogen and Oxygen Intermediates in Salmonella enterica Serovar Enteritidis

“…ArcA is the transcriptional regulator, and ArcB is a transmembrane protein that contains receiver and transmitter domains. Signals recognized by ArcB, thought to be membrane potential or metabolites generated during anaerobic growth, may activate the system (4,20,21,(26)(27)(28). Recently, Georgellis et al reported that the redox signals for the ArcAB system are quinones which are membrane-associated electron carriers (21).…”

The CydDC Family of Transporters and Their Roles in Oxidase Assembly and Homeostasis

“…As well as adapting to low oxygen conditions, expression and membrane content of cytochrome bd-I has been shown to increase in response to a variety of stresses, including high temperature (Delaney, Wall, & Georgopoulos, 1993;Wall et al, 1992), high pH (Avetisyan, Dibrov, Semeykina, Skulachev, & Sokolov, 1991), high pressure (Tamegai et al, 2005) and the presence of protonophore-uncouplers (Avetisyan et al, 1991;Bogachev, Murtazina, Shestopalov, & Skulachev, 1995;Bogachev, Murtazina, & Skulachev, 1993). Also, induction of cytochrome bd-I by heat shock and loss of PMF is dependent upon the ArcAB two-component system (Wall et al, 1992).…”

Na+‐driven ATP synthesis in Methanobacterium thermoautotrophicum and its differentiation from H+‐driven ATP synthesis by rhodamine 6G

“…thermoautotrophicum [ 151. In this context it should be mentioned that the requirement of a high concentration of Na' for induction of the Enterococcus hirae Na'-ATPase and for the Na'-motive oxidase of Escherichia coli was reported [22,23]. In different methanogenic bacteria the existence of a sodium cycle has been suggested [9,24,25].…”