Cytochrome <i>c</i> Effect on Respiration of Heart Mitochondria: Influence of Various Factors

Toleikis, Adolfas; Trumbeckaitė, Sonata; Majienė, Daiva

doi:10.1007/s10540-005-2897-2

Cited by 8 publications

(9 citation statements)

References 24 publications

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“…42 In this study (Fig. 4B), the increase of respiration rate in state 2 with pyruvate + malate due to ischemia-reperfusion was not observed when compared with the control data presented in Figure 4A and the usually obtainable control respiration rates of isolated mitochondria.…”

Section: Discussioncontrasting

confidence: 50%

“…43 The high degree (2-fold) of stimulation of respiration by cytochrome c in state 3 in mitochondria isolated from ischemia-reperfusion-treated hearts indicates the damage of the outer membrane of the mitochondria and the possible cytochrome c release from the mitochondria, which often causes state 3 respiration rate decrease. 42,44 However, GBE treatment (neither concentration, 0.05 and 0.4 mL) of rat hearts did not affect the cytochrome c test value, suggesting that GBE did not prevent the damage of outer mitochondrial membrane and cytochrome c loss from mitochondria during the ischemia-reperfusion period (data not shown).…”

Section: Discussionmentioning

confidence: 92%

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Influence of Ethanol Extract of Ginkgo biloba Leaves on the Isolated Rat Heart Work and Mitochondria Functions

Baliūtytė

Banienė

Gendvilienė³

et al. 2012

Journal of Cardiovascular Pharmacology

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In this study, we attempted to elucidate whether the effects of ethanol extract of Ginkgo biloba leaves (GBE) observed previously on isolated rat heart mitochondria may be realized in situ (in case of isolated heart perfused under normal conditions and under ischemia-reperfusion). We found that GBE at low concentrations (0.01, 0.05, and 0.1 mL/mL) does not affect the heart rate and parameters of electrocardiogram (ECG) but produces a small increase in the coronary flow. Higher concentration of GBE (0.2 and 0.3 mL/mL) diminished the heart rate, decreased the coronary flow, and tended to enhance the parameters of ECG. The contractility of isolated rat heart and mitochondrial nicotinamide adenine dinucleotide reduced form fluorescence decreased in a GBE concentration-dependent manner. Mitochondria isolated from hearts pre-perfused with GBE (0.05 mL/mL) for 20 minutes before nonflow global ischemia-reperfusion (45 min/15 min) showed higher respiratory rates with pyruvate + malate in state 2 and state 3, higher respiratory control index, and diminished H 2 O 2 generation compared with untreated group. Higher GBE concentration, 0.4 mL/mL, had no effect on H 2 O 2 generation and did not prevent the ischemiareperfusion-induced decrease of pyruvate + malate oxidation in state 3 but even enhanced it. However, in the case of nonischemic perfusions, this GBE concentration had no significant effect on these parameters of respiratory functions of isolated heart mitochondria.

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Section: Discussioncontrasting

confidence: 50%

Section: Discussionmentioning

confidence: 92%

Influence of Ethanol Extract of Ginkgo biloba Leaves on the Isolated Rat Heart Work and Mitochondria Functions

Baliūtytė

Banienė

Gendvilienė³

et al. 2012

Journal of Cardiovascular Pharmacology

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“…It was found that quercetin at higher concentration (30-50 μM) and in some conditions can do it (Ortega and García 2009) and cause the decrease in mitochondrial respiration rate. To check this possibility we applied cytochrome c test (Toleikis et al 2005). We observed large enhancement of depressed by EGb761 mitochondrial respiration both in the absence and presence of ADP after addition of pure cytochrome c into incubation medium (Figs.…”

Section: Discussionmentioning

confidence: 99%

Effects of standardized extract of Ginkgo biloba leaves EGb761 on mitochondrial functions: mechanism(s) of action and dependence on the source of mitochondria and respiratory substrate

Baliūtytė

Trumbeckaitė

Banienė

et al. 2014

J Bioenerg Biomembr

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In this work, the effects of standardized dry extract of Ginkgo biloba leaves, EGb761, on the respiration of rat heart and liver mitochondria were investigated. We revealed uncoupling of oxidative phosphorylation in rat heart mitochondria by EGb761 which was not observed in liver mitochondria respiring on pyruvate + malate; oxidation of succinate in heart mitochondria was inhibited by EGb761, concentration-dependently, almost completely at 1.00 mg/mL. Uncoupling effect of EGb761 was found to be due to increase in H(+) and K(+) permeability of inner membrane of mitochondria which is most likely to be mediated by the ATP/ADP-translocator and uncoupling proteins. EGb761 depressed State 3 respiration with pyruvate + malate (similarly in heart and liver mitochondria) and succinate (stronger than with pyruvate + malate) but not respiratory chain Complex IV; inhibition of respiration was not restored by uncoupler indicating the inhibitory action of EGb761 on the respiratory complexes preceding to Complex IV and/or on the substrate transport. Moreover, EGb761 rapidly reduced pure cytochrome c. This property of EGb761 together with the observed uncoupling of oxidative phosphorylation and reduction of H2O2 accumulation may be beneficial for the cell in the prevention of apoptosis and protection of cellular functions in pathological situations.

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“…Respiration is ceased when the substrate and ADP are absent (State I), 20 and slightly activated when only the substrate succinate is present (State IV). 5 This translated to the comparable deformation of isolated mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Membrane Deformability and Membrane Tension of Single Isolated Mitochondria

et al. 2008

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Abstract-Mitochondria dynamics is crucial to many biological processes such as mitochondria fusion and fission, which is highly correlated to the mechanics of single mitochondria. However, the mechanobiological coupling of mitochondria has been poorly understood. Here membrane deformability and membrane tension of individual mitochondria isolated from MtDsRed labeled human embryonic T-Rex-293 kidney cells were measured using a micropipette aspiration assay. The results demonstrated that membrane deformation of isolated mitochondria exhibited an elastic transition phase followed by an equilibrium phase, and mitochondrial membrane tension was proportional to the area compressibility. It was also indicated that mitochondrial membrane deformability was significantly affected by physical-chemical factors such as osmotic pressure or pH value, and was further correlated to mitochondrial functionality in different respiratory states and Ca 2+ regulation. These findings provide a new insight into understanding the mechanical regulation of mitochondrial physiology.

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Cytochrome c Effect on Respiration of Heart Mitochondria: Influence of Various Factors

Cited by 8 publications

References 24 publications

Influence of Ethanol Extract of Ginkgo biloba Leaves on the Isolated Rat Heart Work and Mitochondria Functions

Influence of Ethanol Extract of Ginkgo biloba Leaves on the Isolated Rat Heart Work and Mitochondria Functions

Effects of standardized extract of Ginkgo biloba leaves EGb761 on mitochondrial functions: mechanism(s) of action and dependence on the source of mitochondria and respiratory substrate

Membrane Deformability and Membrane Tension of Single Isolated Mitochondria

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