Cytochrome gene expression shifts in Geobacter sulfurreducens to maximize energy conservation in response to changes in redox conditions

Howley, Ethan T; Krajmalnik‐Brown, Rosa; Torres, César I.

doi:10.1016/j.bios.2023.115524

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…Within these organisms, multiple parallel pathways seem to exist, at least as options at the level of the genome. This matches the observation in G. sulfurreducens and S. oneidensis of multiple pathways that offer alternative routes for reduction or oxidation of insoluble acceptors and donors, respectively (66,78), which is often linked to their ability in fine-tuning access to various redox potential ranges and conserve energy (79,80). Ultimately, this underscores the importance of having diverse MHC as a beneficial option to adapt to multiple conditions, as previously proposed (81).…”

Section: Geobacterialessupporting

confidence: 86%

A Survey of theDesulfuromonadiacytochromome provides a glimpse of the unexplored diversity of multiheme cytochromes in nature

Soares,

Fonseca,

Nash

et al. 2024

Preprint

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Multiheme cytochromes (MHC) provide prokaryotes with a broad metabolic versatility that contributes to their role in the biogeochemical cycling of the elements. However, MHC were isolated and studied in detail only from a limited number of species. To obtain a broader view of the diversity of MHC, we employed bioinformatics tools to study the cytochromome encoded in the genomes of theDesulfuromonadiaclass. We found that MHC predicted to be extracellular are the least conserved and present higher diversity. Although the most prevalent MHC have homologues already characterized, nearly half of the MHC families in theDesulforomonadiaclass have no known homologues and AlphaFold2 was employed to predict their 3D structures. This work illuminates for the first time the universe of experimentally uncharacterized cytochromes that are likely to contribute to the metabolic versatility and to the fitness ofDesulfuromonadiain diverse environmental conditions and to drive biotechnological applications.

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Section: Geobacterialessupporting

confidence: 86%

A Survey of theDesulfuromonadiacytochromome provides a glimpse of the unexplored diversity of multiheme cytochromes in nature

Soares,

Fonseca,

Nash

et al. 2024

Preprint

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Influence of support materials on the electroactive behavior, structure and gene expression of wild type and GSU1771-deficient mutant of Geobacter sulfurreducens biofilms

Rodríguez-Torres,

Huerta-Miranda,

Martínez-García

et al. 2024

Environ Sci Pollut Res

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Geobacter sulfurreducens DL1 is a metal-reducing dissimilatory bacterium frequently used to produce electricity in bioelectrochemical systems (BES). The biofilm formed on electrodes is one of the most important factors for efficient electron transfer; this is possible due to the production of type IV pili and c-type cytochromes that allow it to carry out extracellular electron transfer (EET) to final acceptors. In this study, we analyzed the biofilm formed on different support materials (glass, hematite (Fe2O3) on glass, fluorine-doped tin oxide (FTO) semiconductor glass, Fe2O3 on FTO, graphite, and stainless steel) by G. sulfurreducens DL1 (WT) and GSU1771-deficient strain mutant (Δgsu1771). GSU1771 is a transcriptional regulator that controls the expression of several genes involved in electron transfer. Different approaches and experimental tests were carried out with the biofilms grown on the different support materials including structure analysis by confocal laser scanning microscopy (CLSM), characterization of electrochemical activity, and quantification of relative gene expression by RT-qPCR. The gene expression of selected genes involved in EET was analyzed, observing an overexpression of pgcA, omcS, omcM, and omcF from Δgsu1771 biofilms compared to those from WT, also the overexpression of the epsH gene, which is involved in exopolysaccharide synthesis. Although we observed that for the Δgsu1771 mutant strain, the associated redox processes are similar to the WT strain, and more current is produced, we think that this could be associated with a higher relative expression of certain genes involved in EET and in the production of exopolysaccharides despite the chemical environment where the biofilm develops. This study supports that G. sulfurreducens is capable of adapting to the electrochemical environment where it grows.

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Impact of extended starvation conditions on bioelectrocatalytic activity of a methane-producing microbial electrolysis cell

Cristiani,

Zeppilli,

Brutti

et al. 2024

Bioresource Technology

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Cytochrome gene expression shifts in Geobacter sulfurreducens to maximize energy conservation in response to changes in redox conditions

Cited by 3 publications

References 45 publications

A Survey of theDesulfuromonadiacytochromome provides a glimpse of the unexplored diversity of multiheme cytochromes in nature

A Survey of theDesulfuromonadiacytochromome provides a glimpse of the unexplored diversity of multiheme cytochromes in nature

Influence of support materials on the electroactive behavior, structure and gene expression of wild type and GSU1771-deficient mutant of Geobacter sulfurreducens biofilms

Impact of extended starvation conditions on bioelectrocatalytic activity of a methane-producing microbial electrolysis cell

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