Cytochrome C with Peroxidase‐like Activity Supported on Plasmonic AuNPs: Improved Stability and Enhanced Nanobioplasmonic Catalytic Conversion

Abstract

Metallic nanoparticles have been extensively used to adsorb biomolecules and improve their stability under broader conditions. This study focuses on the interaction of Cytochrome C (CytC) and gold nanoparticles (AuNP), CytC@AuNP. Circular dichroism (CD) and Raman show significant differences in secondary structure, mainly in α‐helix. CD ramp temperatures revealed enhanced thermal stability of CytC@AuNP, as evidenced by a refolding of the α‐helix conformation of CytC and a delay in denaturation temperature by approximately 15 °C. Catalytic oxidation performed with CytC@AuNP showed a hyperbolic profile up to 104 % higher yields compared to CytC, which exhibited a linear profile and 30 % loss of activity after thermal treatment. The same reaction performed upon plasmon stimulus comparatively increased the catalytic yield of CytC@AuNP in 12 %, although it had no effect on free CytC. This study highlights the advantages of adsorbing biomolecules on nanoparticles and is a glimpse at plasmon‐driven nanobiocatalysis.

Unraveling the Nano–Bio Interface Interactions of a Lipase Adsorbed on Gold Nanoparticles under Laser Excitation

“…In particular, the effects generated from the localized surface plasmon resonance (LSPR) excitation of plasmonic NPs can lead to enhanced performance of biocatalysts coupled to their surface, which has gained significant relevance as a novel strategy to improve biocatalysis. , This emerging field of study, recently named plasmonic biocatalysis, explores the improvement of biocatalytic performance by remote regulation of enzyme properties via noninvasive strategies . Previous studies have shown the effect of laser stimuli on biocatalytic activity using different enzymes and supports. − However, although these studies analyzed the interactions between enzymes and metallic nanoparticles, the changes in their secondary structure and enzyme–nanoparticle interactions induced by laser irradiation have largely been underexplored. However, the impacts of LSPR excitation at the nano–bio interface need to be further studied.…”