Cytochrome c dissolved in 1‐allyl‐3‐methylimidazolium chloride type ionic liquid undergoes a quasi‐reversible redox reaction up to 140°C

Tamura, Kaori; Nakamura, Nobuhumi; Ohno, Hiroyuki

doi:10.1002/bit.24357

Cited by 47 publications

(42 citation statements)

References 50 publications

(53 reference statements)

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“…This occurs even though the ILs have a different affinity owing to the anionic structures. Despite the fact that [BMI]Cl has a high β value and [BMI] [Tf 2 N] has a low β value [28], both ILs form a cholesterol-water interface. These observations suggest that [EMI][Lac] and [Ch][Lac] will also localize around the lipid-water interface in a vesicle solution.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Hydrophilic Ionic Liquids 1-Ethyl-3-Methylimidazolium Lactate and Choline Lactate on Lipid Vesicle Fusion

et al. 2013

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Ionic liquids (ILs) are room-temperature molten salts that have applications in both physical sciences and more recently in the purification of proteins and lipids, gene transfection and sample preparation for electron microscopy (EM) studies. Transfection of genes into cells requires membrane fusion between the cell membrane and the transfection reagent, thus, ILs may be induce a membrane fusion event. To clarify the behavior of ILs with cell membranes the effect of ILs on model membranes, i.e., liposomes, were investigated. We used two standard ILs, 1-ethyl-3-methylimidazolium lactate ([EMI][Lac]) and choline lactate ([Ch][Lac]), and focused on whether these ILs can induce lipid vesicle fusion. Fluorescence resonance energy transfer and dynamic light scattering were employed to determine whether the ILs induced vesicle fusion. Vesicle solutions at low IL concentrations showed negligible fusion when compared with the controls in the absence of ILs. At concentrations of 30% (v/v), both types of ILs induced vesicle fusion up to 1.3 and 1.6 times the fluorescence intensity of the control in the presence of [Ch][Lac] and [EMI][Lac], respectively. This is the first demonstration that [EMI][Lac] and [Ch][Lac] induce vesicle fusion at high IL concentrations and this observation should have a significant influence on basic biophysical studies. Conversely, the ability to avoid vesicle fusion at low IL concentrations is clearly advantageous for EM studies of lipid samples and cells. This new information describing IL-lipid membrane interactions should impact EM observations examining cell morphology.

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Section: Discussionmentioning

confidence: 99%

The Effect of Hydrophilic Ionic Liquids 1-Ethyl-3-Methylimidazolium Lactate and Choline Lactate on Lipid Vesicle Fusion

et al. 2013

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“…[1][2][3] Furthermore, as the first research group, which converted the chemical oscillation of a BZ reaction into a mechanical change in polymer gels, we have been systematically studying a "self-oscillating gel" that exhibits an autonomous swelling-deswelling oscillation under nonoscillatory outer conditions. [15] Ohno and coworkers also found that cytochrome c retained 70 % of its original activity for a long period of time (as long as 18 months) in a mixture of choline dihydrogen phosphate PIL and water, [16] known as a "hydrated IL". [10][11][12] These properties are a high ion conductivity, non-flammability, (electro)chemical stability, and thermal stability.…”

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confidence: 99%

“…[13] ILs can be divided generally into two categories: ILs with an ion structure that includes active protons (protic ILs: PILs) and that does not include active protons (aprotic ILs: APILs). [15] Ohno and coworkers also found that cytochrome c retained 70 % of its original activity for a long period of time (as long as 18 months) in a mixture of choline dihydrogen phosphate PIL and water, [16] known as a "hydrated IL". PILs are assumed to be a promising class of a preservation medium for biopolymers.…”

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confidence: 99%

Belousov–Zhabotinsky Reaction in Protic Ionic Liquids

2012

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Rhythmic oscillation is one of the universal phenomena in living systems, and important rhythms that sustain a life, such as heart beating, breathing, and circadian rhythm, occur at all levels in physiological tissue. The Belousov-Zhabotinsky (BZ) reaction is an oscillating reaction accompanying a rhythmical oscillation of the redox potential of metal catalysts, such as ferroin and ruthenium bipyridine, Ru(bpy) 3 . Typically, the BZ reaction is caused in a strong acidic aqueous solution using sulfuric acid or HNO 3 as proton source with an oxidant (for example, sodium bromate, NaBrO 3 ) and an organic acid acting as the reductant (for example, malonic acid, MA, or citric acid). The rhythmic self-oscillation of the BZ reaction is known to have many analogies to living systems and is important from the perspective of understanding nonlinear, non-equilibrium science. [1][2][3] Furthermore, as the first research group, which converted the chemical oscillation of a BZ reaction into a mechanical change in polymer gels, we have been systematically studying a "self-oscillating gel" that exhibits an autonomous swelling-deswelling oscillation under nonoscillatory outer conditions. [4][5][6][7][8][9] However, to apply this gel to functional materials, there are two problems that have to be solved: 1) the BZ reaction occurs only under strong acidic conditions (a typically pH value is below one) and 2) the duration of the operation is short.Ionic liquids (ILs) are room-temperature molten salts and have attracted much attention because of their interesting properties, which render them useful for applications in materials science. [10][11][12] These properties are a high ion conductivity, non-flammability, (electro)chemical stability, and thermal stability. [13] ILs can be divided generally into two categories: ILs with an ion structure that includes active protons (protic ILs: PILs) and that does not include active protons (aprotic ILs: APILs). [14] Recently, many research groups have pointed out the affinity between living matter and (P)ILs. PILs are assumed to be a promising class of a preservation medium for biopolymers. Byrne and Angell reported that a hen egg protein recovered from a thermally denatured structure to a significant higher-order architecture by using a PILs/water mixture as the solvent. [15] Ohno and coworkers also found that cytochrome c retained 70 % of its original activity for a long period of time (as long as 18 months) in a mixture of choline dihydrogen phosphate PIL and water, [16] known as a "hydrated IL". MacFarlane and coworkers reported long-term structural and chemical stabilities of DNA in hydrated PILs. [17] Excellent review articles are now available on (P)ILs for biopolymers. [18,19] Studies on the preparation of ILs from bioderivatives also received attention. [20,21] On the other hand, for the development of energy conversion and storage materials, PILs are widely seen as a promising novel proton-conducting medium for fuel cell applications, instead of the traditional acidic aqueous soluti...

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“…Polar ILs exhibit extremely high hydrogen bond basicity 14 and are reported to be good solvents for some poorly soluble materials such as polysaccharides and proteins. 13,57 The second-generation polar ILs, namely phosphonate-and carboxylate-type ILs, have recently gained special importance owing to their high polarity and low viscosity as compared to those of chloride-type ILs. 2,3,8 Since cellulose displays high crystallinity and poor solubility, many studies have reported enzymatic hydrolysis of cellulose after pretreatment with polar ILs.…”

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confidence: 99%

“…11,12 While reports show that only chloride-type ILs can dissolve cytochrome c (cyt c) without denaturation, 13 phosphonate-type ILs cause its denaturation. On the other hand, Fujita et al reported that certain ILs, typically composed of choline dihydrogen phosphate and a small volume of water, enhance protein stabilization without denaturation in the presence of a small volume of water.…”

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confidence: 99%

Renaturation of Cytochrome c Dissolved in Polar Phosphonate-type Ionic Liquids Using Highly Polar Zwitterions

2017

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Cytochrome c dissolved in 1‐allyl‐3‐methylimidazolium chloride type ionic liquid undergoes a quasi‐reversible redox reaction up to 140°C

Cited by 47 publications

References 50 publications

The Effect of Hydrophilic Ionic Liquids 1-Ethyl-3-Methylimidazolium Lactate and Choline Lactate on Lipid Vesicle Fusion

The Effect of Hydrophilic Ionic Liquids 1-Ethyl-3-Methylimidazolium Lactate and Choline Lactate on Lipid Vesicle Fusion

Belousov–Zhabotinsky Reaction in Protic Ionic Liquids

Renaturation of Cytochrome c Dissolved in Polar Phosphonate-type Ionic Liquids Using Highly Polar Zwitterions

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