Cytochrome c biosensor for determination of trace levels of cyanide and arsenic compounds

Fuku, Xolile; Iftikar, Faiza; Hess, Euodia; Iwuoha, Emmanuel I.; Baker, Priscilla

doi:10.1016/j.aca.2012.02.025

Cited by 55 publications

(23 citation statements)

References 15 publications

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“…The control disc with no MT adsorbed showed As 3+ -dependent peaks at approximately +160 mV (Figure 4) which is very similar to that reported in the literature with more sensitive electrodes [40,41,42]. The potential of As 3+ -stripping in the MT-modified disc scans was shifted to approximately −60 mV and this type of shift is consistent with coordination by thiol [43] and protein modified electrodes [44]. Both the control and experimental traces had high degrees of linearity over the range tested (Table 1).…”

Section: Resultssupporting

confidence: 86%

A Simple Metallothionein-Based Biosensor for Enhanced Detection of Arsenic and Mercury

2017

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Metallothioneins (MTs) are a family of cysteine-rich proteins whose biological roles include the regulation of essential metal ions and protection against the harmful effects of toxic metals. Due to its high affinity for many toxic, soft metals, recombinant human MT isoform 1a was incorporated into an electrochemical-based biosensor for the detection of As3+ and Hg2+. A simple design was chosen to maximize its potential in environmental monitoring and MT was physically adsorbed onto paper discs placed on screen-printed carbon electrodes (SPCEs). This system was tested with concentrations of arsenic and mercury typical of contaminated water sources ranging from 5 to 1000 ppb. The analytical performance of the MT-adsorbed paper discs on SPCEs demonstrated a greater than three-fold signal enhancement and a lower detection limit compared to blank SPCEs, 13 ppb for As3+ and 45 ppb for Hg2+. While not being as low as some of the recommended drinking water limits, the sensitivity of the simple MT-biosensor would be potentially useful in monitoring of areas of concern with a known contamination problem. This paper describes the ability of the metal binding protein metallothionein to enhance the effectiveness of a simple, low-cost electrochemical sensor.

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Section: Resultssupporting

confidence: 86%

A Simple Metallothionein-Based Biosensor for Enhanced Detection of Arsenic and Mercury

2017

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“…12 For example, genetically engineered bacterial cells, 13 and enzyme inhibition assays were developed to detect arsenic. 14,15 On the chemical sensor side, a few optical and electrochemistry assays were reported. [16][17][18][19] The World Health Organization (WHO) initially sets a guideline for arsenic in drinking water at 50 µg/L (0.67 µM).…”

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confidence: 99%

DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection

2014

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“…Similarly, subcutaneous and intraperitoneal injection of 100 nm NDs in rats led to accumulation of NDs in retention organs over 1 month with no impact on the rats' viability [197]. However, a slight surface-dependent genotoxicity of NDs was recently reported on embryonic stem cells [199].…”

Section: Nds Toxicity and Biodistributionmentioning

confidence: 98%

Novel Aspects of Diamond

2015

Topics in Applied Physics

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Cytochrome c biosensor for determination of trace levels of cyanide and arsenic compounds

Cited by 55 publications

References 15 publications

A Simple Metallothionein-Based Biosensor for Enhanced Detection of Arsenic and Mercury

A Simple Metallothionein-Based Biosensor for Enhanced Detection of Arsenic and Mercury

DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection

Novel Aspects of Diamond

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