Cytochrome C Based Superoxide Sensor for In Vivo Application

Scheller, W; Jin, Wen; Ehrentreich‐Förster, Eva; Ge, Bao-Feng; Lisdat, Fred; Büttemeier, R.; Wollenberger, Ursula; Scheller, Frieder W.

doi:10.1002/(sici)1521-4109(199907)11:10/11<703::aid-elan703>3.0.co;2-j

Cited by 83 publications

(48 citation statements)

References 23 publications

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“…Recently, single component T-COOH momolayers 58 and mixed-monolayers of short alkanethiols 59 with immobilized cyt c gold electrodes have been employed in the electrochemical detection of superoxide radical.…”

Section: ·2 Application To Biosensing Of Metabolismic Materials Inmentioning

confidence: 93%

Electrochemical Biosensors in Nonaqueous Solutions and Their Applications

Nakamura

2007

ANAL. SCI.

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This review first describes the invention of functional interfaces to promote biochemical redox reactions between substrates in dipolar aprotic solvents and enzymes or related compounds immobilized at the interface. The interfaces contain hydrophilic polymer membranes, a gold nanoparticle self-assembled electrode constructed by using rigid rod dithiols, and binary self-assembled monolayers composed of amino and carboxyl terminal groups. Other topics covered are: the electrochemical characterization of the hydrophilic polymer membrane; the development of biosensors to obtain reaction parameters of enzymatic and of electrochemical kinetics; and applications to the study of materials involved in metabolism.

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Section: ·2 Application To Biosensing Of Metabolismic Materials Inmentioning

confidence: 93%

Electrochemical Biosensors in Nonaqueous Solutions and Their Applications

Nakamura

2007

ANAL. SCI.

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“…5. The O2 -radical generated in the biopolymer membrane would be electrocatalytically oxidized by 14 ANALYTICAL SCIENCES JANUARY 2001, VOL. 17 the immobilized cyt c to give an anodic current response.…”

Section: Amperometric Measurement Of Superoxide Radical and Sodmentioning

confidence: 99%

“…[1][2][3][4][5][6] Detection of superoxide and SOD has been intensively pursued by spectrophotometric and electrochemical methods. [7][8][9][10][11][12][13][14][15][16] Electrochemical sensors are of great interest due to their advantages, which include microfabrication and in-vivo and in-vitro applications. 17,18 The hemoprotein, cytochrome c (cyt c), undergoes facile reductive reaction with superoxide.…”

Section: Introductionmentioning

confidence: 99%

“…Electrochemical biosensors for superoxide with cyt c as the sensing element were constructed employing a suitable electron-transfer promoter. [11][12][13][14] SOD is a selective scavenger of superoxide, and the best way for the detection of SOD thus could be the estimation of superoxide. Superoxide undergoes spontaneous disproportionation to produce H2O2, an electroactive species.…”

Section: Introductionmentioning

confidence: 99%

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Amperometric Detection of Superoxide Dismutase at Cytochrome c-Immobilized Electrodes: Xanthine Oxidase and Ascorbate Oxidase Incorporated Biopolymer Membrane for in-vivo Analysis

Gobi

Mizutani

2001

ANAL. SCI.

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Superoxide radical is highly reactive and short-lived and is formed in all aerobic cells. The superoxide radical generated during the "respiratory burst" of phagocytic cells plays an important role in the destruction of microorganisms invading into our body. But it may be involved in oxidative damage to tissues and organisms if it exceeds the level at which the systems are able to provide defence. The ubiquitous presence of SOD enzyme throughout the evolutionary chain emphasizes the importance of superoxide radical in cell function and survival. SOD plays a major protective role in living cells and has been widely used as a pharmacological tool in the study of pathophysiological mechanisms. 1-6 Detection of superoxide and SOD has been intensively pursued by spectrophotometric and electrochemical methods. [7][8][9][10][11][12][13][14][15][16] Electrochemical sensors are of great interest due to their advantages, which include microfabrication and in-vivo and in-vitro applications. 17,18 The hemoprotein, cytochrome c (cyt c), undergoes facile reductive reaction with superoxide. Electrochemical biosensors for superoxide with cyt c as the sensing element were constructed employing a suitable electron-transfer promoter.11-14 SOD is a selective scavenger of superoxide, and the best way for the detection of SOD thus could be the estimation of superoxide. Superoxide undergoes spontaneous disproportionation to produce H2O2, an electroactive species. Thus, the electrical response of the superoxide sensor system for interfering compounds is a very important limitation in its application. We recently reported a selective detection of superoxide and SOD at cyt c/alkanethiol electrodes, where the response due to interferents was suppressed by the use of a mixed-monolayer of alkanethiols for anchoring cyt c; but the presence of L-ascorbic acid, a potential scavenger of superoxide existing ubiquitously in our body, was detrimental to the detection of superoxide and SOD. 19 The detection of superoxide and consequently SOD in the presence of L-ascorbic acid was established using XOD/cyt c-bilayer electrodes, where the poly-L-lysine: polystyrenesulfonate complex membrane employed for the immobilization of XOD restricted the permeation of L-ascorbic acid across the membrane and consequently suppressed the scavenging of the superoxide produced at the membrane-bound XOD by Lascorbic acid. 20 The XOD/cyt c-bilayer electrodes could detect SOD in the presence of L-ascorbic acid; however, the sensitivity of XOD/cyt c-bilayer electrodes for SOD was rather low (detection limit, 0.2 µg ml -1 ) relative to that of cyt c-monolayer electrodes (detection limit, 3 ng ml -1 ); 19,20 the permeation of SOD across the polyion complex membrane confining XOD was suppressed in the case of the bilayer electrodes, which resulted into a low sensitivity for the detection of SOD. In the present investigation, the cyt c-immobilized monolayer electrodes were prepared by covalently-immobilizing cyt c on mixed-monolayers of 3-mercaptopropionic acid (MPA) with t...

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“…Although several electrochemical biosensors for the separate measurement of O 2 •-and H 2 O 2 have been presented (Alpeeva et al, 2005;Campanella et al, 2000Campanella et al, , 1997Emreguel, 2005;Ferapontova et al, 2001;Ge and Lisdat, 2002;Lindgren et al, 2000;Lisdat et al, 1999;McNeil et al, 1995;McNeil and Manning, 2002;Scheller et al, 1999;Shleev et al, 2006), only a limited number of studies describe their simultaneous use (Krylov et al, 2006;Lvovich and Scheeline, 1997;Shipovskov et al, 2004). Furthermore, to our best knowledge simultaneous optical (using LumH 2 ) and electrochemical (using biosensors) monitoring of O 2 •-and H 2 O 2 production by human neutrophil granulocytes has not been performed.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous use of electrochemistry and chemiluminescence to detect reactive oxygen species produced by human neutrophils

Shleev

Wetterö

Magnusson

et al. 2008

Cell Biology International

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A novel approach for the simultaneous optical and electrochemical detection of biologically produced reactive oxygen species has been developed and applied. The set-up consists of a luminol-dependent chemiluminescence assay combined with two amperometric •-sensitive amperometric response. By contrast, the H 2 O 2 sensitive biosensor, based on an HRP-modified graphite electrode, was able to reflect the bulk concentration of H 2 O 2 , produced by stimulated neutrophils, and would be very useful in modestly equipped biomedical research laboratories. In summary, the system would also be appropriate for assessment of several other metabolites in different cell types, and tissues of varying complexity, with only minor electrode modifications. _____________________________________________________________________________

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Cytochrome C Based Superoxide Sensor for In Vivo Application

Cited by 83 publications

References 23 publications

Electrochemical Biosensors in Nonaqueous Solutions and Their Applications

Electrochemical Biosensors in Nonaqueous Solutions and Their Applications

Amperometric Detection of Superoxide Dismutase at Cytochrome c-Immobilized Electrodes: Xanthine Oxidase and Ascorbate Oxidase Incorporated Biopolymer Membrane for in-vivo Analysis

Simultaneous use of electrochemistry and chemiluminescence to detect reactive oxygen species produced by human neutrophils

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