Cytochrome b gene for species identification of the conservation animals

Hsieh, Hsing Mei; Chiang, Hsiao Ling; Tsai, Li Chin; Lai, Shu Ya; Huang, Nu En; Linacre, Adrian; Lee, James Chun-I

doi:10.1016/s0379-0738(01)00403-0

Cited by 188 publications

(133 citation statements)

References 9 publications

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“…17 Few studies are also reported using scanning electron micrography of rhinoceros horn. 21,34 Identified sculptors made up of rhinoceros horn using SEM and microscopic characteristics to use it for molecular study. 8 In present study scanning electron microscopy was used to study both dorsal and ventral surface and the structure noticed were distinct that is presence of compact hair growth dorsally as seen in Figure 2a and presence of uniformly placed pores having mean diameter of 320 µm and these range from 160 to 400 µm on the ventral surface as shown in Figure 3a.…”

Section: Discussionmentioning

confidence: 99%

“…Hair like structure was also noticed by. 34 Presence of sub-pores having diameter 9.39 µm and these ranges from 5.56 to 10.11 µm within a pore is very remarkable and can be used for identification of rhinoceros horn as seen in Figure 3c. These pores seem to be similar to the tubules examined by Hieronymus et al The pattern of "sub-pores within a pore" is unique to rhinoceros horn and it is very difficult to imitate it with uniformity thus, it can be important tool for forensic identification.…”

Section: Discussionmentioning

confidence: 99%

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Scanning electron microscopy: a potential forensic tool to identify a piece of rhinoceros horn

Singh¹,

Saini

Goyal

2017

Int J Sci Rep

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Background: Illegal trade of rhinoceros horn in the global market has posed a serious threat to the long-term survival and the conservation of endangered one horn rhinoceros. Presence of fake horns imitating rhinoceros horn makes the situation graver. Thus, to curb the trade it is very important to scientifically identify the horns used in trade to support and strengthen the enforcement agencies. The presence of fake items makes the identification task much more difficult thus, a project entitled “Characterization of species from bone, tusk, rhino horn and antler to deal wildlife offence cases” was developed to identify rhinoceros (Rhinoceros unicornis) horn in complete to pulverized form.Methods: Various bio-scientific techniques like morphological, analytical and molecular were applied to identify the rhinoceros horn. Most of these techniques are promising in identifying rhinoceros horn in different forms. In present paper, we discuss on identification of a piece of rhinoceros horn using Scanning Electron Microscope and total nine horns were examined. The required micrographs were saved using software Digital Image Scanning System (DISS 5, Point Electronics, Germany). Scanned images for both rhinoceros horn from wild and zoo were similar. To find the specific signature, the micrographs of the rhinoceros horn were compared with buffalo and fake horns. Results: Differences were noted on both dorsal and ventral surfaces of rhinoceros horns examined under scanning electron microscope. On ventral surface numerous uniformly placed circular pores with mean diameter of 320 µm and had characteristic “sub pores within a pore”. Instead, in fake horn only few non-uniform pores were visible without any sub pores. The ventral portion of buffalo horn does not indicate presence of any such pores.Conclusions: The characteristic “sub pores within a pore” signature of the ventral portion of rhinoceros horn has high potential for forensic identification and consequently, proving offences and convicting offenders.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Scanning electron microscopy: a potential forensic tool to identify a piece of rhinoceros horn

Singh¹,

Saini

Goyal

2017

Int J Sci Rep

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“…(F5'-TAAA-CTTCAGGTGACCAAAAAATCA-3') 8 Diversas regiões do DNA mitocondrial podem ser utilizadas na identificação de espécies animais, entre elas, o gene Cytocromo b (cytb), que tem sido uma escolha tradicional para este fim desde sua descoberta 22,23 . Na literatura, podem ser encontrados inúmeros exemplos do uso desta região na identificação de vertebrados para fins forenses, incluindo mamíferos 24 , peixes 25 ; répteis 26 , e aves 27,28 , inclusive seus ovos [29][30][31] .…”

Section: Hco2198unclassified

Identificação de Espécies de Animais Silvestres por Meio de Sequências Mitocondriais Utilizados no Combate aos Crimes Contra a Fauna – Mato Grosso – Brasil

Carmo¹,

Garcez²,

Vasconcelos³

et al. 2017

BJFS

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Resumo. O Brasil possui uma importância inquestionável no cenário da biodiversidade global o que o torna um dos países mais visados para este que é o terceiro maior comércio ilícito do mundo, o tráfico de animais silvestres. Mesmo com o reconhecimento da técnica molecular como importante ferramenta na identificação, ainda paira uma discussão sobre qual o melhor segmento de DNA a ser utilizado Cytb ou COI e se estes são efetivos para amostras degradadas, como é o caso das amostras forenses. Objetivo foi contribuir para dissolução dos questionamentos e depositar no banco de dados GenBank ® as sequências de DNA mitocondrial geradas. As

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“…However, these methods are of limited use in species identification. The progress of molecular biology introduced a new approach, which is based on nucleotide sequence diversities among species in particular regions of DNA [5][6][7]. The nucleotide regions chosen for species identification were varied by researchers.…”

Section: Introductionmentioning

confidence: 99%

“…The nucleotide regions chosen for species identification were varied by researchers. Within vertebrates, a cytochrome b (cyt b) gene in the mitochondrial DNA has been studied from multiple viewpoints including the nucleotide diversity among species [6] and the availability of nucleotide sequence data for references [5]. Many of the other regions studied are also located in the mtDNA.…”

Section: Introductionmentioning

confidence: 99%