Cytochemistry of mouse mast cell reaction to polylysine

Abstract

The modifications of mouse mast cells incubated in solutions of polylysine at two different concentrations are studied. At higher dose (0.5 mg/ml), the polycation has evident toxic effects. At lower dose (0.5 micrograms/ml), it induces some modifications of the granule morphology. Cytochemical reactions to demonstrate polyanions, basic proteins and organic bases are used to study the granule content. A polylysine treatment promotes the release of basic groups (vaso-amines) and sulphydryl-containing compounds (basic protein) from the mast cell granules. On the contrary, polyanions remain in the cells and correspond to the granule matrix persisting after amino-liberator treatment.

Impact of Actin Rearrangement and Degranulation on the Membrane Structure of Primary Mast Cells: A Combined Atomic Force and Laser Scanning Confocal Microscopy Investigation

“…As an alternative stimulation approach, poly-L-lysine (152689, MW 1500-8000; MP Biomedicals, Solon, OH) coated onto coverslips was used. The degranulation of mast cells triggered by poly-L-lysine has been reported by previous studies (29)(30)(31). Our goal was to demonstrate the sensitivity of combined AFM and LSCM to the variations in cell signaling, and to capture the characteristic membrane and cytoskeletal structures of BMMCs under this protocol.…”

Phenomenology of hadron structure — why low energy physics matters

“…In Ref. [21] the perturbative evolution approach is justified by comparing it to the nonperturbative momentum dependence as determined by the phenomenon of the freezing of the coupling constant, and to analyze the consequences of introducing an effective gluon mass.…”

“…The main experimental access to the tensor and scalar charges is provided thanks to the sum rules Eqs. (20,21). The knowledge on the PDFs h 1 (x) and e(x), up to theoretical limitations, will bring some light on the values of g S/T .…”

Inactivation of herpes simplex virus types 1 and 2 by synthetic histidine peptides

“…In early studies, investigators examined the effect of different molecular species of poly-L-lysine polypeptides on a variety of viruses. The viruses studied included tobacco mosaic, mumps, Newcastle disease, and influenza, and although inhibitory effects were noted in both tissue culture and animal studies, poly-L-lysine has since been determined to be toxic in mammalian systems (1,4,27,28).In our laboratory, we have been examining the antifungal properties of synthetic homologous histidine polypeptides (24, 25) because of their structural and functional resemblance to a unique family of naturally occurring histidinerich polypeptides secreted by the human salivary glands (17,24,26). In the course of our investigations into the spectrum of antimicrobial activities of these synthetic histidine peptides, we have observed that poly-L-histidine of appropriate chain length and molecular weight can directly and irreversibly inactivate herpes simplex virus types 1 and 2 (HSV-1 and HSV-2).…”

“…In early studies, investigators examined the effect of different molecular species of poly-L-lysine polypeptides on a variety of viruses. The viruses studied included tobacco mosaic, mumps, Newcastle disease, and influenza, and although inhibitory effects were noted in both tissue culture and animal studies, poly-L-lysine has since been determined to be toxic in mammalian systems (1,4,27,28).…”