Cytochemical study of interaction between lymphocytes and target cells in tissue culture

Sura, S. N.; Chernyakhovskaya, I. Yu.; Kadaghidze, ZairaG.; Fuks, B. B.; Svet-Moldavsky, GeorgeJ.

doi:10.1016/0014-4827(67)90338-2

Cited by 26 publications

(4 citation statements)

References 5 publications

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“…Lindholm and Britton [20] have found morphologic "bridges" between several mammalian cells. Moreover, Sura et al [21] claim that bridges form between lymphocytes and target cells in tissue culture (with transfer of DNA), but we have not been able t o demonstrate such bridges in our system. However, connections such as those seen by Schoenberg We have used prelabeling with vital dyes to identify target and aggressor cells, which can occasionally overlap in size.…”

Section: Discussioncontrasting

confidence: 70%

Intercellular communication in cell‐mediated cytotoxicity. Fluorescein transfer between H‐2^d target cells and H‐2^b lymphocytes in vitro

1971

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Fluorescein permeable junctions occur infrequently between unsensitized C57BL/6 lymphocytes (H‐2b) and DBA/2 mastocytoma cells (H‐2d), but the incidence of such junctions increases significantly with lymphocytes from animals sensitized against the mastocytes. The mastocytoma cells were labeled intracellularly with fluorescein using permeant (non‐fluorescent) fluorescein dipropionate which is hydrolyzed to free fluorescein within the cells; they were additionally stained with neutral red. As an in vitro model of cell‐mediated cytotoxicity the Brunner system was used in which close contact is a prerequisite of cell lysis. With contacts of aggressor cells to targets fluorescein permeable junctions were found in 17 out of 23 experiments, or 168 times, when sensitized spleen cells from female C57BL mice took part in the reaction. This incidence is significantly greater than in control experiments with normal spleen cells, when fluorescein permeable junctions were observed only 28 times and in only 9 out of 23 experiments.

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Section: Discussioncontrasting

confidence: 70%

Intercellular communication in cell‐mediated cytotoxicity. Fluorescein transfer between H‐2^d target cells and H‐2^b lymphocytes in vitro

1971

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“…Although most evidence in support of their proposal comes from ultrastructural studies, there have been other suggestions for exterioration and transfer of components from killer to target cell. For example, cytoplasmic junctions between CTL and TC suggesting a mechanism for transfer of a lysogeneic component has been reported by Sura et al (1967) and by Sellin et al (1971). These investigators observed transfer of cytoplasmic contents or fluorescent probes.…”

Section: Transfer Of Materials From Ctl To the Target Cellmentioning

confidence: 77%

Cytotoxic T‐Lymphocytes How Do They Function?

Berke

1983

Immunological Reviews

114

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The central theme of this work has been the roles of the CTL receptor and of MHC-proteins in CTL recognition and lysis. A major conclusion that may be deduced from the work presented here is that one CTL receptor is responsible for both target cell recognition and lysis. Although their function as recognitive structures is well established, involvement of MHC-proteins in the events that follow recognition has not been investigated in detail. We have proposed that MHC-proteins are molecular mediators whereby CTL receptors transmit signals ultimately leading to lysis of the target cell. I see future work on CTL-mediated lysis proceeding in the following directions: 1. Verification and analysis of the precise role of MHC proteins in CTL recognition and lysis by use of cell and vesicle systems of defined composition and structure. 2. Study of CTL-mediated 'lethal hit' in systems enabling analysis of early events (millisecond level) preceding lysis. 3. Grafting of CTL receptor(s) activity onto naive cells, using liposomes or other vehicles, and 4. Production of idiotypic reagents such as monoclonal antibodies specific for the combining site/effector mechanism of CTL.

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“…Another possible mechanism of target cell destruction -penetration into them of lymphocyte nucleic acids -has not been proved, even though such penetration has been established (Sura et al 1967). In a recent study using electron microscopy it was shown that the so-called "nuclear bridge" between the lymphocyte and the target cell was the result of phagocytosis of fragments of dead lymphocytes, and DNA of lymphocytes phagocytized by the target cell had no functional activity (Sura el al.…”

Section: Immediate Consequences Of Immunological Recognitionmentioning

confidence: 99%

Lymphocyte Receptors and Mechanisms of in Vitro Cell‐Mediated Immune Reactions

Brondz

1972

Immunological Reviews

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Cytochemical study of interaction between lymphocytes and target cells in tissue culture

Cited by 26 publications

References 5 publications

Intercellular communication in cell‐mediated cytotoxicity. Fluorescein transfer between H‐2^d target cells and H‐2^b lymphocytes in vitro

Intercellular communication in cell‐mediated cytotoxicity. Fluorescein transfer between H‐2^d target cells and H‐2^b lymphocytes in vitro

Cytotoxic T‐Lymphocytes How Do They Function?

Lymphocyte Receptors and Mechanisms of in Vitro Cell‐Mediated Immune Reactions

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Cytochemical study of interaction between lymphocytes and target cells in tissue culture

Cited by 26 publications

References 5 publications

Intercellular communication in cell‐mediated cytotoxicity. Fluorescein transfer between H‐2d target cells and H‐2b lymphocytes in vitro

Intercellular communication in cell‐mediated cytotoxicity. Fluorescein transfer between H‐2d target cells and H‐2b lymphocytes in vitro

Cytotoxic T‐Lymphocytes How Do They Function?

Lymphocyte Receptors and Mechanisms of in Vitro Cell‐Mediated Immune Reactions

Contact Info

Product

Resources

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Intercellular communication in cell‐mediated cytotoxicity. Fluorescein transfer between H‐2^d target cells and H‐2^b lymphocytes in vitro

Intercellular communication in cell‐mediated cytotoxicity. Fluorescein transfer between H‐2^d target cells and H‐2^b lymphocytes in vitro