Cytochemical Staining and Immunocytochemistry

Raskin, Rose E.; Santangelo, Kelly S.; Polak, Klaudia

doi:10.1002/9781119500537.ch61

Cited by 4 publications

(10 citation statements)

References 154 publications

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“…The eosinophils were strongly positive for PAS and showed a diffusely stained magenta-coloured cytoplasm when stained for the same. This finding was similar to the finding of Mohd et al (2015) in Uttara fowl but was different from the finding of Raskin (2010), who reported that the eosinophils of chickens were negative for PAS. The cytoplasmic granules of the heterophils were moderately positive for PAS, which was in accordance to the finding of Kumar (2019) in domestic fowl but completely opposite to the findings of Thral et al (2004) where they reported that the avian heterophils were negative for PAS.…”

Section: Discussionsupporting

confidence: 62%

“…All the erythrocytes, basophils, lymphocytes, monocytes and thrombocytes examined under this study were negative for SBB, which was similar to the findings of Shalini (2015) in domestic fowl and of Chen et al (2019) in domestic pigeon. However, Salakij et al (2003) had reported that erythrocytes of Painted storks were positive for SBB while Raskin (2010) reported that the avian monocytes were occasionally positive for SBB.…”

Section: Discussionmentioning

confidence: 99%

“…However, Andreasen and Latimer (1990) reported that the heterophils and eosinophils of chicken were both negative for alkaline phosphatase. Raskin (2010) reported that in dog and cat the late stages of neutrophils are devoid of alkaline phosphatase activity. In contrast, enzymatic activity is present in the myeloblast and promyelocyte stages, so the demonstration of alkaline phosphatase activity was helpful in the diagnosis of myelogenous leukaemia.…”

Section: Discussionmentioning

confidence: 99%

“…However, Bounous and Stedman (2000) reported that the heterophils of avian species were negative, but the eosinophils were positive for the enzyme acid phosphatase while Claver and Quaglia (2009) reported that heterophils and eosinophils of avian species were both positive for the enzyme acid phosphatase. Raskin (2010) reported that acid phosphatase activity may be used to detect various blast cells and may help diagnose acute myeloblastic leukaemia like myelomonocytic leukaemia, erythroleukemia and megakaryoblastic leukaemia.…”

Section: Discussionmentioning

confidence: 99%

“…However, Salakij et al. (2003) had reported that erythrocytes of Painted storks were positive for SBB while Raskin (2010) reported that the avian monocytes were occasionally positive for SBB. Jamal (2020) reported that SBB is a fat‐soluble dye that stains fat particles in the primary and secondary granules of myelocytic and monocytic cells.…”

Section: Discussionmentioning

confidence: 99%

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Light microscopic morphology of blood cells of non‐descript indigenous Zoar chicken of Mizoram, India

Doley,

Sarma,

Kalita

et al. 2024

Anat Histol Embryol

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Identifying and analysing distinct blood cells is crucial for the diagnosis and treatment of diseases in the field of biomedicine. The present study was undertaken to study the cytomorphological and cytochemical characteristics of the blood cells of Zoar, a non‐descript indigenous breed of chicken extensively reared under backyard poultry farming in Mizoram, India. For this study, 2 mL of blood samples were aseptically collected from the wings veins of 12 chickens and were processed for light microscopic study under standard protocols. The matured erythrocytes were elliptical, while the immature erythrocytes appeared oval. The heterophils were positive for SBB (SBB), Periodic Acid Schiff (PAS), acid phosphatase, alkaline phosphatase and Arylsulphatase while the eosinophils were positive for SBB, PAS, alkaline phosphatase, cytochrome oxidase and peroxidase. The basophils of were positive for toluidine blue while the thrombocytes were positive for PAS. These cytochemical and cytoenzymatic staining properties plays a very important role in diagnosis, differentiation, and classification of leukaemias.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Light microscopic morphology of blood cells of non‐descript indigenous Zoar chicken of Mizoram, India

Doley,

Sarma,

Kalita

et al. 2024

Anat Histol Embryol

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Evaluation of canine and feline leukocyte differential counts obtained with the scil vCell 5 compared to the Advia 2120 hematology analyzer and a manual method

Zelmer,

Moritz,

Bauer

2023

J VET Diagn Invest

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The vCell 5 (scil Animal Care), a point-of-care hematology analyzer (POCA), was recently introduced to veterinary laboratories. This laser- and impedance-based analyzer is capable of providing a CBC with 5-part WBC differential count (Diff) along with WBC cytograms and flags serving as interpretation aids for numerical results. We compared the scil POCA-Diff to reference methods (i.e., manual differential count, Advia 2120 hematology analyzer [Siemens]) for canine and feline blood samples and considered WBC cytograms and flags. Total observed error (TEo), calculated from CV and bias%, was compared to total allowable error (TEa). Data were analyzed before and after a review process (exclusion of flagged and samples with invalid cytograms). For both species, correlation was good-to-excellent ( rs = 0.81–0.97) between both analyzers for all variables, except for feline monocytes ( rs = 0.21–0.63) and canine monocyte% ( rs = 0.50). Smallest biases were seen for neutrophils (dog: −5.7 to 0.8%; cat: 1.5–9.4%) with both reference methods. Quality requirements (TEo < TEa) were fulfilled for canine and feline neutrophils (TEo = 5.3–10.6%, TEa = 15%) and eosinophils (TEo = 67.1–83%, TEa = (90)–50%) considering at least one reference method. Our review process led to mildly higher rs-values for most variables. Although not completely satisfactory, the scil POCA provides reliable results in compliance with ASVCP quality goals for canine and feline neutrophils and eosinophils. Analyzer flag and cytogram analysis served as useful tools for QA, indicating the necessity for manual review of blood smears, and contributed to improvement of scil POCA performance.

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Indicaciones para la evaluación morfológica de la médula ósea en caninos y felinos: revisión de la literatura

Roldán–Carvajal,

Jaramillo–Arbeláez,

Velásquez

et al. 2023

Rev. Med. Vet. Zoot.

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Son múltiples las indicaciones para evaluar la médula ósea de caninos o felinos, entre ellas, anomalías persistentes o de causa no explicable, alteraciones en el hemogramay/o en la bioquímica sanguínea, acompañadas de signos y síntomas que pueden ser inespecíficos, estos pueden ser trastornos reactivos o de origen medular. El objetivo de esta revisión es presentar un panorama general de las indicaciones más frecuentes para la evaluación morfológica de la médula ósea en caninos y felinos y hallazgos reportados en la literatura. Como metodología, se empleó un abordaje de los trastornos malignosy benignos con compromiso medular en caninos y felinos, mediante una búsqueda de artículos originales en las bases de datos PubMed, ScienceDirect, Scopus, SciELO y LILACS, en la que se utilizaron los términos MeSh: bone marrow neoplasms, bone marrow examination, congenital bone marrow failure syndromes, bone marrow failure disorders, combinados con el operador booleano AND con las palabras dog y cat. Se encontraron artículos que estudiaron la médula ósea para el diagnóstico diferencial de infeccionescomo leishmaniasis, ehrlichiosis y anaplasmosis. Sin embargo, en su mayoría, las publicacionestenían como objetivo el diagnóstico de procesos malignos, como trastornos mieloproliferativos y linfoproliferativos. Se concluye que la evaluación de la médula ósea es de gran importancia, lo que permite esclarecer diagnósticos y evaluar la estadificación de algunas enfermedades, así como su pronóstico y seguimiento.

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Cytochemical Staining and Immunocytochemistry

Cited by 4 publications

References 154 publications

Light microscopic morphology of blood cells of non‐descript indigenous Zoar chicken of Mizoram, India

Light microscopic morphology of blood cells of non‐descript indigenous Zoar chicken of Mizoram, India

Evaluation of canine and feline leukocyte differential counts obtained with the scil vCell 5 compared to the Advia 2120 hematology analyzer and a manual method

Indicaciones para la evaluación morfológica de la médula ósea en caninos y felinos: revisión de la literatura

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