Cytochemical reactions of normal and starving Amoeba proteus

Heller, Ida M.; Kopac, M. J.

doi:10.1016/0014-4827(56)90204-x

Cited by 7 publications

(4 citation statements)

References 23 publications

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“…Many new compounds synthesized in the course of such investigations have proven useful in this and other laboratories for studies of inhibitory activities, enzyme susceptibilities, identifications, spectra (33,56,113), etc. Proof of structure by synthesis has been offered for several natural nucleosides (70,78,106,110,141,170,207), and several nucleosides first synthesized here as potential antimetabolites were subsequently found in nature, most notably, all of the major methylated nucleosides of the tRNA's.…”

Section: Rbe Of Fast Neutrons On Sea Urchin Eggsmentioning

confidence: 99%

“…Organic chemical techniques for nucleoside syntheses (66,81,106,141,198,199), isopropylidene derivatives (172), thiations of nucleosides (181,207,268), and nucleotide syntheses (173, 259) have been derived from these works. Many new compounds synthesized in the course of such investigations have proven useful in this and other laboratories for studies of inhibitory activities, enzyme susceptibilities, identifications, spectra (33,56,113), etc.…”

Section: Rbe Of Fast Neutrons On Sea Urchin Eggsmentioning

confidence: 99%

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Biological effects of radiation, and related biochemical and physical studies. Summary progress report, July 1, 1950--September 30, 1971

Wade¹

1971

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Section: Rbe Of Fast Neutrons On Sea Urchin Eggsmentioning

confidence: 99%

Section: Rbe Of Fast Neutrons On Sea Urchin Eggsmentioning

confidence: 99%

Biological effects of radiation, and related biochemical and physical studies. Summary progress report, July 1, 1950--September 30, 1971

Wade¹

1971

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“…ly fluid and compressible (40), contain peripherally lipoprotein (40,10,47) and, in the inner core, Mg and Ca (30,31). Recent studies on Amoeba proteus using electron probe analysis (19) have shown that Mg, Ca, K, and P are present throughout the HSBs.…”

Section: Introductionmentioning

confidence: 99%

“…Several authors (38,63,3) have ascribed variations in the number of HSBs to the species of prey organism, but no detailed studies relating cell growth, and division rate, during a given diet, to the number and size of the inclusions have yet been made. Data so far accumulated on HSBs show that they are the heaviest organelles in the cell (41,54,2), soluble in water (31), present a two-phase appearance in the light microscope, are partial-* Footnote: The abbreviation ~HSBs~ will be used to designate the heavy spherical bodies throughout this paper.…”

Section: Introductionmentioning

confidence: 99%

Studies on the heavy spherical (refractive) bodies of freshwater Amoebae. I. Morphology and regeneration of HSBS in Chaos carolinense

Chapman-Andresen

1976

Carlsberg Res. Commun.

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*Supported by The Carlsberg FoundationThe heavy spherical bodies of Amoeba and Chaos are refractive organelles, varying in size from ca. 10 ~tm in diameter, down to the limit of resolution of the light microscope. These inclusions have many characteristics in common with the phosphate-rich, Jvolutin~ granules of other unicellular organisms, but differ from the latter in being constantly present in healthy growing amoebae, while in other organisms they appear only under conditions of nutritional imbalance. The fine structure of the HSBs of Amoeba and Chaos show that the organelles are membrane-bound, with an electron translucent periphery, and an electron dense inner core which sublimes in the electron beam, but which is stabilized by lead staining. During centrifugation in vivo, the HSBs collect at the centrifugal pole of the amoebae; under favourable conditions, a HSB-sack is formed at the heavy pole, and can be excised, leaving amoebae, normal in other respects, but containing only ca. 5% of the normal complement of HSBs. Using this method for obtaining practically HSB-free amoebae, the regeneration of HSBs was studied in Chaos carolinense, by determining the number and size distribution of HSBs in a standard volume in fixed, whole mounts by phase contrast microscopy. It was found that although the number of HSBs per standard volume in both fed and starved amoebae returned to that found in control, unoperated, Chaos by 7 days after operation, the size distribution did not return to normal until ca. 50 days after excision of the HSBs, indicating that HSBs originate from precursors in the cytoplasm. Operated amoebae showed normal feeding and locomotory behaviour, and their division rate was similar to that of control amoebae. However, HSB-free amoebae were more prone to rupture at an air-water interface, presumably owing to their lower specific gravity.

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Localization of ionic calcium in

Shida

1970

Experimental Cell Research

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Cytochemical reactions of normal and starving Amoeba proteus

Cited by 7 publications

References 23 publications

Biological effects of radiation, and related biochemical and physical studies. Summary progress report, July 1, 1950--September 30, 1971

Biological effects of radiation, and related biochemical and physical studies. Summary progress report, July 1, 1950--September 30, 1971

Studies on the heavy spherical (refractive) bodies of freshwater Amoebae. I. Morphology and regeneration of HSBS in Chaos carolinense

Localization of ionic calcium in

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