Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining

Chi, Heng; Wen, Li-Lian; Sui, Zhi-Hai; Sun, Qinglei; Sun, Li

doi:10.1016/j.tice.2017.10.008

Cited by 9 publications

(8 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human MPO is also found in monocytes in mammals (Olsson et al, 2004). However, this does not seem to be the case in turbot, as monocytes were described as peroxidase negative (Chi et al, 2017). However, because myeloperoxidase has been reported in macrophages of some fish species (Meseguer et al, 1994;Neumann et al, 2000), the presence of Mpx in other turbot cell types cannot be ruled out, as the sensitivity of the cytochemical methods is relatively low.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the turbot Scophthalmus maximus (L.) myeloperoxidase. An insight into the evolution of vertebrate peroxidases

Noia

Fontenla-Iglesias

Valle

et al. 2021

Developmental & Comparative Immunology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Characterization of the turbot Scophthalmus maximus (L.) myeloperoxidase. An insight into the evolution of vertebrate peroxidases

Noia

Fontenla-Iglesias

Valle

et al. 2021

Developmental & Comparative Immunology

View full text Add to dashboard Cite

“…Although the expression of MPO in monocytes has been reported, it still is a matter of debate. Chi et al., 2017 reported that a potassium iodide and oxidized pyronine Y (KI-PyY) stained MPO + cells in turbot exhibited characteristics of neutrophil morphology ( 51 ). Other markers used to differentiate different types of cells are: Zap-70 (Zeta-chain-associated protein kinase 70) a phylogenetically conserved protein used to visualize the presence of both T and NK lymphocytes ( 33 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of myeloperoxidase and its contribution to antimicrobial effect on extracellular traps in flounder (Paralichthys olivaceus)

Gan

Chi²,

Dalmo³

et al. 2023

Front. Immunol.

Self Cite

View full text Add to dashboard Cite

Myeloperoxidase (MPO) is a cationic leukocyte haloperoxidase and together with other proteins, they possess activities against various microorganisms and are involved in extracellular trap (ET) formation. The present work describes the gene and deduced protein sequences, and functions of MPO in flounder (PoMPO). The PoMPO possesses a 2313 bp open reading frame (ORF) that encodes a protein of 770 amino acids. The highest PoMPO mRNA expression levels were found in the head kidney, followed by peritoneal cells, gill, spleen, skin, muscle, and liver. PoMPO was expressed in MHCII+ and GCSFR+ cells which indicated that PoMPO mainly is expressed in flounder macrophages and granulocytes. Bacterial lipopolysaccharide-stimulated peritoneal leukocytes showed an increased protein level of PoMPO while it seemed that LPS also promoted the migration of MPO+ cells from the head kidney into the peripheral blood and peritoneal cavity. After phorbol 12-myristate 13-acetate (PMA) or bacterial stimulation, flounder leukocytes produced typical ET structures containing DNA with decoration by MPO. The ETs containing DNA and PoMPO effectively inhibited the proliferation of ET-trapped bacteria. Blocking PoMPO with antibodies decreased the enzymatic activity, which attenuated the antibacterial activity of ETs. This study pinpoints the involvement of ETs in flounder innate responses to pathogens.

show abstract

“…Chi et al. reported that MPO-positive cells probably represented neutrophils with a high proportion (38.05%) in the peritoneum of turbot stained by potassium iodide and oxidized pyronine Y ( Chi et al., 2017 ). Other investigators have reported that MPO is not detectable after the differentiation of monocytes into macrophages ( Kumar et al., 2004 ).…”

Section: Discussionmentioning

confidence: 99%

Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)

Chi

Shi

et al. 2022

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

Adjuvants are used to increase the strength, quality, and duration of the immune response of vaccines. Neutrophils are the first immune cells that arrive at the injection site and can release DNA fibers together with granular proteins, so-called neutrophil extracellular traps (NETs), to entrap microbes in a sticky matrix of extracellular chromatin and microbicidal agents. Similar extracellular structures were also released by macrophages, mast cells, and eosinophils and are now generalized as “ETs.” Here we demonstrated that Alum adjuvant stimulation led to peritoneal cells swarming and ET release in vitro. Moreover, compared to antigen stimulation alone, ET release was significantly increased after stimulation with antigen-mixed adjuvants and in a time- and dose-dependent manner. In vivo, we were able to monitor and quantify the continuous changes of the ET release in the same fish by using the small animal in vivo imaging instrument at different times during the early stages after intraperitoneal immunization. The results showed that the fluorescence signal of ETs in the peritoneum increased from 0 to 12 h after injection and then gradually decreased. The fluorescence signals came from extracellular DNA fibers, which are sensitive to DNase I and confirmed by microscopy of peritoneal fluid ex vivo. In summary, this study introduced a new method for detecting ETs in the peritoneum of fish in vivo and indicated that ET formation is involved in the immune response at the early stage after intraperitoneal immunization to vaccines.

show abstract

Cytochemical identification of turbot myeloperoxidase-positive granulocytes by potassium iodide and oxidized pyronine Y staining

Cited by 9 publications

References 25 publications

Characterization of the turbot Scophthalmus maximus (L.) myeloperoxidase. An insight into the evolution of vertebrate peroxidases

Characterization of the turbot Scophthalmus maximus (L.) myeloperoxidase. An insight into the evolution of vertebrate peroxidases

Characterization of myeloperoxidase and its contribution to antimicrobial effect on extracellular traps in flounder (Paralichthys olivaceus)

Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)

Contact Info

Product

Resources

About