Cytochemical detection of saccharide residues in paramural bodies formed in tomato root cells infected by Fusarium oxysporum f. sp. radicis-lycopersici

Chamberland, H.; Benhamou, Nicole; Ouellette, G. B.; Pauzé, F. J.

doi:10.1016/0885-5765(89)90021-0

Cited by 14 publications

(10 citation statements)

References 30 publications

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“…This internal layer also contains traces of pectic compounds. Other cytochemical studies conducted with an exoglucanase-gold complex also indicate that this method is easily usable and gives reliable and intense labelling patterns (Chamberland et al 1989;Nicole et al 1992;Nicole et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Suberized tyloses in trees: An ultrastructural and cytochemical study

Rioux

Chamberland

Simard

et al. 1995

Planta

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The nature of the wall layers observed in suberized tyloses was studied in Populus basalmifera L., Ulmus americana L. and Quercus rubra L. As the suberin layers were present only in tyloses that had completed their expansion, most of the results concern mature tyloses. The cyto-and immunocytochemical tests were conducted, respectively, with an exoglucanase having a binding affinity for 13(l~4)-D-glucans, the subunits of cellulose, and with two monoclonal antibodies specific for un-esterified and esterified pectic molecules. In the three species, labelling for pectic compounds was intense over the external layer of tyloses but usually more dispersed or nearly absent over the layer corresponding to a primary wall that was, however, intensely labelled for 13(1 ~4)-Dglucans. The outer wall layer, comparable to a middle lamella in mature tyloses, was continuous with similar material that appeared to be secreted by the tylosis. This material was particularly abundant in pit chambers, in void spaces between the tylosis and the vessel wall, particularly at the junction of the vessel and two adjacent cells, and close to the rim of vessel perforation plates. In P. balsamifera, a single suberized layer or occasionally a succession of suberized and cellulose-containing layers was observed internal to the tylosis primary wall. In U. americana, the wall of tylosis was similar to that of P. balsamifera except that, at times, a secondary-wall-like layer was formed and only a single suberized layer was observed. In Q. rubra, the suberized layer was always observed internal to the tylosis secondary wall. Simple pits were also constantly noted in Q. rubra tyloses. The occasional occurrence of a cellulosic layer internally to the suberized layer was observed in the three species. Histochemical tests revealed that lignin was also an important component of the tylosis wall. The tyloses frequently contained phenolic compounds in close association with the suberized layers. The significance of the formation of suberized tyloses in trees is discussed.Abbreviation: TEM = transmission electron microscope

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Section: Discussionmentioning

confidence: 99%

Suberized tyloses in trees: An ultrastructural and cytochemical study

Rioux

Chamberland

Simard

et al. 1995

Planta

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“…2 F). Interestingly, the composition of saccharide residues transported in paramural bodies seems to be different from the material secreted into papillae (see below) as Gal, GalNAc, GalA, and [~-glucosides were identified as a the major constituents (Chamberland et al 1989). The separation of the necrotic lesion involves not only physical strengthening of the walls but also disconnection of protoplasmic continuity between cells.…”

Section: Plant Defence Responses To Fungal Infectionmentioning

confidence: 98%

Pathogenic infection and the oxidative defences in plant apoplast

et al. 2001

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The structural and functional continuum of the plant apoplast is the first site of contact with a pathogen and plays a crucial role in initiation and coordination of many defence responses. In this paper, we present an overview of the involvement of the plant apoplast in plant-pathogen interactions. The process of infection of French bean (Phaseolus vulgaris L.) plants by Colletotrichum lindemuthianum is analysed. The ultrastructural features of plant defence responses to fungal infection are then compared with those observed in plants or cell suspensions treated with various elicitors. Changes in cell walls and in whole plant cells responding to infection seem to be highly similar in all systems used. Model systems of French bean and white lupin (Lupinus albus L.) are then utilised to provide some biochemical characteristics of oxidative reactions in the apoplast evoked by elicitor treatment. The species specificity of various mechanisms generating reactive oxygen species is discussed, and some details of pH-dependent H2O2-generating activity of peroxidases are demonstrated. As its exocellular nature is an important feature of the oxidative burst, the major consequence of this event, i.e., the oxidative cross-linking of wall components during the papilla formation and strengthening of the walls, is analysed. Finally, the possible involvement of other wall-associated and developmentally regulated H2O2-generating mechanisms, like amine and oxalate oxidases, in plant defence is demonstrated. It is concluded that under stress conditions, such apoplastic mechanisms might be employed to increase plants' chances of survival.

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“…The Ricinus communis agglutinin I (Rc A,) -gold complex was prepared according to Benhamou et al (1988b) (pH 8.0) and applied for the localization of D-galactose residues. Helixpomatia agglutinin (HpA) was utilized for the detection of the N-acetyl-D-galactosamine residues and complexed to colloidal gold at pH 7.4 as previously described (Chamberland et al 1989). Concanavalin A (ConA) was used for the detection of a-D-mannose and (or) glucose and complexed to colloidal gold at pH 8.0, while Ulex europaeus agglutinin 1 (Ue A,) was applied for localization of a-L-fucose residues after being complexed to colloidal gold at pH 6.3 (Chamberland et al 1989).…”

Section: Tissue Processingmentioning

confidence: 99%

“…Application of the HpA-gold complex for localization of N-acetyl-D-galactosamine residues (Chamberland et al 1989) resulted in a specific labeling of the cytoplasm of chlamydospores (Fig. 12).…”

Section: Cytochemical Labeling Of N-acetyl-o-galactosamine Residuesmentioning

confidence: 99%

Cytochemical localization of some polysaccharide components in the cell walls of Chalara elegans during its life cycle

Dumas‐Gaudot¹,

Tahiri-Alaoui²,

Benhamou³

1992

Can. J. Microbiol.

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DUMAS-GAUDOT, E., TAHIRI-ALAOUI, A., and BENHAMOU, N. 1992. Cytochemical localization of some polysaccharidic components in the cell walls of Chalara elegans during its life cycle. Can. J. Microbiol. 38: 828-837. The occurrence of polysaccharides and sugars in the cell walls of the soil-borne pathogenic fungus Chalara elegans (deuteromycete) has been investigated at the electron microscope level, using cytochemical approaches based on the affinity of lectin or enzyme-gold complexes for some carbohydrates. Evidence for the presence of both P-1,4-glucans and chitinous components is reported here for the first time in the cell walls of chlamydospores, endoconidia, and hyphae. A minor component with N-acetyl-D-galactosamine residues is also detected in the cell walls and, as a storage product, in the cytoplasm. The spatial distribution of both cellulosic P-1,4-glucans and N-acetylglucosamine residues differs according to the fungal cells, and the present results give a more accurate description of the multilayered structure of the fungal walls (i.e., chlamydospores and hyphae).

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Cytochemical detection of saccharide residues in paramural bodies formed in tomato root cells infected by Fusarium oxysporum f. sp. radicis-lycopersici

Cited by 14 publications

References 30 publications

Suberized tyloses in trees: An ultrastructural and cytochemical study

Suberized tyloses in trees: An ultrastructural and cytochemical study

Pathogenic infection and the oxidative defences in plant apoplast

Cytochemical localization of some polysaccharide components in the cell walls of Chalara elegans during its life cycle

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