Cytochemical Demonstration of Acrosomal Proteinase in Mammalian and Avian Spermatozoa by a Silver Proteinate Method

Yanagimachi, Ryuzo; Teichman, R.

doi:10.1093/biolreprod/6.1.87

Cited by 75 publications

(29 citation statements)

References 21 publications

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“…They also provide conclusive evidence for the lack of uniformity or homogeneity of the acrosome. In all the species studied, the acrosomal collar (Yanagimachi & Noda, 1970) remains resistant to the extraction. The acrosomal cap substance shows considerable variation, both in ease of extraction and in the enzymes associated with this substance.…”

Section: Light Microscopic Examinationmentioning

confidence: 96%

“…There are now a number of exceptions to the lysosome-like character of the acrosome, largely re¬ lating to the expectation of the presence of a number of hydroly tic enzymes with acid pH optima. The trypsin-like enzyme (Zaneveld, Srivastava & Williams, 1969) (Teichman & Bernstein, 1971) (Teichman, 1969) (Yanagimachi & Teichman, 1972 (Fig. 5), man (Fig.…”

Section: Light Microscopic Examinationmentioning

confidence: 99%

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A Chemical Procedure for Extraction of the Acrosomes of Mammalian Spermatozoa

Bernstein¹,

Teichman²

1973

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Section: Light Microscopic Examinationmentioning

confidence: 96%

Section: Light Microscopic Examinationmentioning

confidence: 99%

A Chemical Procedure for Extraction of the Acrosomes of Mammalian Spermatozoa

Bernstein¹,

Teichman²

1973

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“…They reside in the acrosome and are believed to aid the spermatozoon to penetrate the surrounding cells and zona pellucida of the egg before fertilization. The enzymes found in this part of the mammalian (including human) spermatozoon include acid phosphatase, arylsulphatase, N-acetyl-/?-D-glucosaminidase, phospholipase A, proteases (Allison & Hartree, 1970;Teichman & Bernstein, 1971;Penn, Gledhill & Darzynkiewicz, 1972; Yanagimachi & Teichman, 1972), trypsin-like enzyme, 'corona-penetrating' enzyme, neuraminidase Pedersen, 1972;Polakoski, 1972) and hyaluronidase (Srivastava, Adams & Hartree, 1965). Acrosomal proteolytic activity has also been demonstrated in the spermatozoa of Rana pipiens .…”

Section: Introductionmentioning

confidence: 99%

The Distribution of Some Hydrolytic Enzymes in the Semen of the Domestic Fowl, Gallus Domesticus

McIndoe¹,

Lake²

1974

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Summary. The distribution of some hydrolytic enzymes in fowl semen was studied. Washed spermatozoa and seminal plasma were examined for the following enzymes: acid phosphatase, sulphatase, N-acetyl-\g=b\-dglucosaminidase, \g=b\-glucuronidase,phospholipase A, trypsin-like enzyme and neuraminidase. Only a trypsin-like enzyme, N-acetyl-\g=b\-d-glucosaminidase and acid phosphatase were detected in the semen. Most of the acid phosphatase activity occurred in particulate material in the seminal plasma, whereas virtually all the trypsin-like activity was in the spermatozoa. The N-acetyl-\g=b\-d-glucosaminidaseactivity in semen occurred both in the spermatozoa and the seminal plasma; the proportion of the activity in whole semen contributed by the spermatozoa was less than that contributed by the seminal plasma but the concentration of activity was greater in the spermatozoa.A slightly inhibitory action of seminal plasma on the activity of the trypsin-like enzyme was indicated.A preliminary examination was made of the semen of different strains of fowl and revealed between-strain differences in the activity of enzymes, particularly acid phosphatase.

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“…From histochemical studies on the light microscopic level, Yanagimachi and Teichman (1972), by using the silver proteinate method, considered that acrosomal proteinase exists only in the acrosomal cap. However, Garner et al (1975) recognized the presence of the enzyme both in the acrosomal cap and collar by staining the testis with fluorescein-labeled anti-acrosomal proteinase.…”

mentioning

confidence: 99%