Cytochemical characterization of Triatoma infestans and Panstrongylus megistus salivary gland cells (Hemiptera, Reduviidae, Triatominae)

Anhê, Ana Carolina Borella Marfil; Azeredo-Oliveira, Maria Tercília Vilela de

doi:10.1016/j.micron.2008.06.003

Cited by 13 publications

(18 citation statements)

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“…Orcein (for proteins-DNA complexed), Feulgen reaction (specific to DNA) and C-Banding (for constitutive heterochromatin), which are specific for chromatin staining, showed a predominance of decondensed chromatin, as already described for salivary glands of other Hemiptera (laCoMBe, 1999;anHê & azereDo-oliVeira, 2008;serrão et al, 2008;kuMar & saHayaraj, 2012;De Castro et al, 2013;Martínez et al, 2014). These characteristics indicated increasing nuclear mass in relation to the cytoplasm and increasing metabolic activity (li et al, 2007), necessary to produce saliva.…”

Section: Discussionsupporting

confidence: 64%

“…They showed a predominance of two bulky and polyploid nuclei in each cell, as observed in salivary gland of Triatoma infestans (Klug, 1834) and Panstrongylus megistus (Burmeister, 1835) (anHê & azereDo-oliVeira, 2008). According to BartH (1954), this increasing of nuclear mass may provide the intense metabolism, essential to accelerate and regulate cellular regeneration after saliva secretion.…”

Section: Discussionmentioning

confidence: 91%

“…Similar results were found in other species of triatomines. In T. infestans, a heterochromatic body called chromocenter (Mello, 1971(Mello, , 1975) is characteristic of males (anHê & azereDo-oliVeira, 2008) and corresponds to the packed three largest autosomes and the sex chromosome (solari, 1979;Panzera et al, 1992;Perez et al, 1997). In P. megistus, just males presented a heterochromatic corpuscle (anHê & azereDo-oliVeira, 2008), which is characteristic of Y chromosome and can also be present in X1 and X2 chromosomes (tartarotti & azereDo-oliVeira, 1999).…”

Section: Discussionmentioning

confidence: 99%

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Cytochemical study of Rhodnius neglectus and Rhodnius prolixus salivary gland cells (Hemiptera, Triatominae)

Anhê

Lima-Oliveira

Azeredo-Oliveira

2014

Iheringia, Sér. Zool.

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ABSTRACT. Triatomines are hematophagous bugs of medical interest in South and Central America, where they may act as invertebrate hosts of the hemoflagellate protozoa Trypanosoma cruzi (the causative of Chagas' disease) and Trypanosoma rangeli (Tejera, 1920). Triatomines of Rhodnius genus have salivary gland formed by two close and independent units: the principal and the accessory. This gland secretes saliva that abounds in substances that facilitate and permit feeding. Despite this importance, there are few reports on its cytochemistry. In purpose of amplifying this understanding, in this work it was investigated the nuclear structures (chromatin and nucleolar corpuscles) of salivary gland cells of Rhodnius neglectus (Lent, 1954) and Rhodnius prolixus (Stål, 1859). The salivary glands were removed from adult insects, fixed and submitted to different cytochemical methods: lacto-acetic orcein, silver ion impregnation, Feulgen reaction, Toluidine Blue, Variant method of critical electrolyte concentration and C-banding. The results evidenced predominance of binucleated cells, with bulky and polyploid nucleus, decondensed chromatin and a large nucleolar area. In addition, cytoplasmic metachromasy and a clear association between nucleolar and heterochromatic corpuscles were observed. Such characteristics were associated with intense synthesis activity to produce saliva. Besides, the heterochromatic corpuscles observed with C Banding permitted the differentiation of sexes and species.KEYWORDS. Gland, morphology, chromatin, nucleolus, nucleus. (Tejera, 1920). Os triatomíneos do gênero Rhodnius possuem glândula salivar formada por duas unidades próximas e independentes: a principal e a acessória. Esta glândula secreta uma saliva que contém substâncias que facilitam e permitem a hematofagia. Entretanto, apesar da sua importância, poucos estudos realizaram sua análise citoquímica. No propósito de ampliar esse entendimento, neste trabalho foram investigadas as estruturas nucleares (cromatina e corpúsculos nucleolares) das células da glândula salivar de Rhodnius neglectus (Lent, 1954) e Rhodnius prolixus (Stål, 1859). As glândulas foram removidas de insetos adultos, fixadas e submetidas a diferentes métodos citoquímicos: Orceína Lacto-Acética, Impregnação por Íons de Prata, Reação de Feulgen, Azul de Toluidina, Variante da Concentração Eletrolítica Crítica e Bandamento C. Os resultados evidenciaram predomínio de células binucleadas, com núcleos volumosos e poliplóides, cromatina descondensada e uma grande área nucleolar. Além disso, foi observada metacromasia citoplasmática e uma clara associação entre os corpúsculos nucleolares e heterocromáticos. Tais características foram associadas às atividades de intensa síntese proteica para a produção de saliva. Além disso, os corpúsculos heterocromáticos observados no Bandamento C permitiram a diferenciação dos sexos e das espécies. RESUMO. Estudo citoquímico de células da glândula salivar de Rhodnius neglectus e Rhodnius prolixus (Hemiptera, Triatominae) Os triatomíneos são in...

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Cytochemical study of Rhodnius neglectus and Rhodnius prolixus salivary gland cells (Hemiptera, Triatominae)

Anhê

Lima-Oliveira

Azeredo-Oliveira

2014

Iheringia, Sér. Zool.

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“…These structures have been studied anatomically, histologically (Lacombe, 1999), cytogenetically (Anhê and Azeredo-Oliveira, 2008;Anhê et al, 2014), and biochemically (Anhê et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Alkaline phosphatase activity in salivary gland cells of Rhodnius neglectus and R. prolixus (Hemiptera, Triatominae)

et al. 2016

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ABSTRACT. Alkaline phosphatase activity was detected in salivary gland cells of the Rhodnius neglectus Lent, 1954, and R. prolixus Stal, 1859, vectors of Trypanosoma cruzi Chagas, 1909 of Chagas disease) and T. rangeli Tejera, 1920 (pathogenic to insect). The Gomori technique was used to demonstrate alkaline phosphatase activity. Alkaline phosphatase activity was observed throughout the entire gland, with an increased activity in the posterior region of the principal gland. In particular, phosphatase activity was found in the nucleolar corpuscles, suggesting a relationship with the rRNA transcription and ribosomal biogenesis. Alkaline phosphatase was also detected in the nuclear membrane and nuclear matrix, suggesting an association with the nucleo-cytoplasmic transport of ribonucleoproteins and the mechanisms of cell cycle and DNA replication, respectively. This study highlights the importance of alkaline phosphatase in the salivary gland of R. prolixus and R. neglectus and emphasizes its importance in secretory activity. Secretory activity is directly involved in hematophagy and, consequently, in development during metamorphosis. The observed presence of alkaline phosphatase suggests its involvement in the production of saliva allowing feeding of these insects that are important vectors of Chagas disease.

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“…Culture supernatants were collected and maintained at -20°C until use. may indicate the higher metabolic activity necessary for rapid and regular cell regeneration (Barth 1954, Anhe & Azeredo-Oliveira 2008.…”

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confidence: 99%

Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells

Rocha

Araújo

Silva

et al. 2010

Mem. Inst. Oswaldo Cruz

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In the present paper, we developed a primary culture of Rhodnius prolixus salivary gland and main salivary canal cells. Cells remained viable in culture for 30 days. Three types of cells were indentified in the salivary gland cultures, with binuclear cells being the most abundant. The supernatants of salivary cultures contained mainly 16-24 kDa proteins and presented anticoagulant and apyrase activities. Secretion vesicles were observed budding from the cellular monolayer of the main salivary canal cells. These results indicate that R. prolixus salivary proteins may be produced in vitro and suggest that the main salivary canal may have a possible secretory role

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Cytochemical characterization of Triatoma infestans and Panstrongylus megistus salivary gland cells (Hemiptera, Reduviidae, Triatominae)

Cited by 13 publications

References 39 publications

Cytochemical study of Rhodnius neglectus and Rhodnius prolixus salivary gland cells (Hemiptera, Triatominae)

Cytochemical study of Rhodnius neglectus and Rhodnius prolixus salivary gland cells (Hemiptera, Triatominae)

Alkaline phosphatase activity in salivary gland cells of Rhodnius neglectus and R. prolixus (Hemiptera, Triatominae)

Primary culture of Rhodnius prolixus (Hemiptera: Reduviidae) salivary gland cells

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