Cytochemical application of tris (2,2'-bipyridine) ruthenium (II):                fluorescence reaction with sulfated polyanions of mast cell granules.

Bertolesi, Gabriel E.; Trigoso, C. I.; Espada, Jesús; Stockert, Juan C.

doi:10.1177/43.5.7730592

Cited by 19 publications

(10 citation statements)

References 5 publications

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“…Rat peritoneal mast cells were isolated essentially as described previously (Berolesi et al, 1999). Adult rats were sacri®ced by ether inhalation followed by cervical dislocation.…”

Section: Isolation and Culture Of Peritoneal Mast Cellsmentioning

confidence: 99%

Effects of mast cells on the behavior of isolated heart fibroblasts: Modulation of collagen remodeling and gene expression

Almeida

Mustin

Forman

et al. 2002

Journal Cellular Physiology

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The extracellular matrix plays a critical role in the development and maintenance of the vertebrate heart. Changes in the accumulation, composition, or organization of the extracellular matrix are known to deleteriously affect heart function. Mast cells are thought to stimulate collagen expression and fibroblast proliferation accompanying fibrosis in some organs; however, the effects of mast cells on the heart interstitium are largely unexplored. The present studies were carried out to determine the effects of mast cells on isolated heart fibroblasts. Several in vitro assays were used including collagen gel contraction to examine the effects of mast cells on the function of isolated fibroblasts. Neonatal heart fibroblasts were cultured either with mast cells, mast cell-conditioned medium, or mast cell extracts, and their ability to contract collagen gels measured. Results from these experiments indicated that mast cells inhibit heart fibroblast migration and contraction of 3-dimensional collagen gels. Further experiments indicated that incubation of neonatal heart fibroblasts with extracts of mast cells altered the expression of collagen, matrix metalloproteases, and matrix receptors of the integrin family. These studies suggest that mast cells play an important role in the regulation of the cardiac interstitial matrix. Further studies are warranted to determine the mechanisms whereby mast cells modulate fibroblast activity.

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“…Rat peritoneal mast cells were isolated essentially as described previously (Berolesi et al, 1999). Adult rats were sacri®ced by ether inhalation followed by cervical dislocation.…”

Section: Isolation and Culture Of Peritoneal Mast Cellsmentioning

confidence: 99%

Effects of mast cells on the behavior of isolated heart fibroblasts: Modulation of collagen remodeling and gene expression

Almeida

Mustin

Forman

et al. 2002

Journal Cellular Physiology

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“…This feature has already been successfully used for very sensitive fluorescence staining of proteins and nucleic acids by different Ru(II) complexes with various chelating heterocyclic ligands in different biochemical samples, e.g. acrylamide gels or Western blot membranes [67,68]. To the best of our knowledge, our approach to prepare the fluorescent Ru(II)-bipyridine complexes in situ in the Ru(II)-metallated tissues is original.…”

Section: Visualization Of Intracellular Ru(ii) By Fluorescence Microsmentioning

confidence: 99%

In vitro and in vivo biological activity screening of Ru(III) complexes involving 6-benzylaminopurine derivatives with higher pro-apoptotic activity than NAMI-A

Trávníček

Matiková-Maľarová

Novotná

et al. 2011

Journal of Inorganic Biochemistry

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“…The former are less acidic than the latter and N-sulfate groups are less acidic than O-sulfates. Considering also that the interaction of Rubipy with O-des and O/N-des N-Ac heparins is lower than with N-des and N-des N-Ac heparins [15], if all the groups were interacting with Rubipy, different Kd should be expected. Our results suggest that not all charged groups bind Rubipy.…”

Section: Heparin-rubipy Binding Characteristicsmentioning

confidence: 99%

“…1) that binds to the negatively charged heparin groups and proved to be very useful for the cytochemical identification of mast cells [15,16]. Rubipy is a fluorescent cationic molecule consisting of one ruthenium atom, which forms a stable chelate-compound with three bipyridine rings.…”

Section: Introductionmentioning

confidence: 99%

Heparan sulfate, heparin, and heparinase activity detection on polyacrylamide gel electrophoresis using the fluorochrome tris(2,2′-bipyridine) ruthenium (II)

Rozenberg¹,

Espada

Cidre

et al. 2001

Electrophoresis

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The paper shows the ability of the fluorochrome tris(2,2'-bipyridine) ruthenium (II) (Rubipy) to detect heparan sulfate, heparin, and heparinase activity of M3 murine mammary adenocarcinoma cells as well as bacterial heparinases I, II, and III in native polyacrylamide gel electrophoresis (PAGE). The technique is based on the electrophoretic mobility of high molecular weight heparins and subsequent staining with Rubipy (50 micrograms/mL). The minimum content of heparin detected by fluorescence in a UV transilluminator was 25-50 ng. The number of Rubipy molecules bound to heparin, determined in relationship to the number of disaccharide units (DU), showed that two to six heparin disaccharide units are bound by each fluorochrome molecule. Scatchard plot analysis showed one Rubipy-binding site (Kd = (8.56 +/- 2.97) x 10(-5) M). Heparinase activity was determined by densitometric analysis of the fluorescence intensity of the heparin-containing band of the gel. While heparinase I (EC 4.2.2.7.) degraded heparin and, to a lower degree, partially N-desulfated N-acetylated heparin (N-des N-Ac), heparinase II (no EC number) could efficiently degrade heparan sulfate (HS) and partially N-des N-Ac heparin. Finally, heparinase III (EC 4.2.2.8.) degraded HS almost exclusively. Only heparin and N-des N-Ac heparin were substrates for M3 tumor cell heparinases. We describe a qualitative, sensitive and simple method to detect heparinase activity and determine its substrate specificity using Rubipy fluorescence with heparin and heparan sulfate in multiple biological samples tested in parallel.

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Cytochemical application of tris (2,2'-bipyridine) ruthenium (II): fluorescence reaction with sulfated polyanions of mast cell granules.

Cited by 19 publications

References 5 publications

Effects of mast cells on the behavior of isolated heart fibroblasts: Modulation of collagen remodeling and gene expression

Effects of mast cells on the behavior of isolated heart fibroblasts: Modulation of collagen remodeling and gene expression

In vitro and in vivo biological activity screening of Ru(III) complexes involving 6-benzylaminopurine derivatives with higher pro-apoptotic activity than NAMI-A

Heparan sulfate, heparin, and heparinase activity detection on polyacrylamide gel electrophoresis using the fluorochrome tris(2,2′-bipyridine) ruthenium (II)

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