Cytochemical and Immunocytochemical Characterization of Yoshida Ascites Sarcoma Cells

Abstract

Some cytochemical and immunocytochemical investigations were carried out on actively growing Yoshida ascites sarcoma cells. These cells displayed an intense granular α-naphthylacetate esterase (ANAE) staining while the α-naphthylbutyrate esterase (ANBE) reaction was in part fluoride-sensitive and marked particularly in the large-sized malignant cells. Acid phosphatase as well as peroxidase activities were not detected. The lack of immu-noreactive lysozyme and α₁-antitrypsin suggested a poor differentiation of the above-mentioned tumor cells, but fibronectin and S-100 protein where highly expressed, as in tumors arising from the mononuclear phagocyte system.