Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa

Martinez, Guillaume; Cappetta, Donato; Telesca, Marialucia; Urbanek, Konrad; Castaldo, Giuseppe; Dhellemmes, Magali; Mele, Vincenza Grazia; Chioccarelli, Teresa; Porreca, Veronica; Barbotin, Anne‐Laure; Boursier, Angèle; Guillou, Florian; Coutton, Charles; Brouillet, Sophie; Angelis, Antonella De; Berrino, Liberato; Pierantoni, Riccardo; Cobellis, Gilda; Chianese, Rosanna; Manfrevola, Francesco

doi:10.7150/ijbs.77166

Cited by 3 publications

(2 citation statements)

References 47 publications

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“…BV2 cells at a density of 2 × 10 6 cells were inoculated into a 60 mm culture dish. After TUS, TMAS, or sham treatment, cells were treated with the phagocytosis inhibitor cytochalasin D (Calbiochem, Saint Louis, MO, USA; Cat#250255, RRID:HY-19693/cs-6968, 10 µM) for 30 min [81], and incubation using HiLyte™ Fluor 555-labeled-Aβ42 oligomers (Anaspec, Fremont, CA, USA; Cat# AS-60480-01, 500 nM) for an additional 4 h followed [82]. Cold PBS-washed cells were used for the detection of Fluor 555-labeled Aβ42 fluorescence under a confocal microscope.…”

Section: Aβ Phagocytosis Assays Of Bv2 Cellsmentioning

confidence: 99%

Transcranial Magneto-Acoustic Stimulation Protects Synaptic Rehabilitation from Amyloid-Beta Plaques via Regulation of Microglial Functions

Zhang,

Tan,

Zhou

et al. 2024

IJMS

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Transcranial magneto-acoustic stimulation (TMAS), which is characterized by high spatiotemporal resolution and high penetrability, is a non-invasive neuromodulation technology based on the magnetic–acoustic coupling effect. To reveal the effects of TMAS treatment on amyloid-beta (Aβ) plaque and synaptic plasticity in Alzheimer’s disease, we conducted a comparative analysis of TMAS and transcranial ultrasound stimulation (TUS) based on acoustic effects in 5xFAD mice and BV2 microglia cells. We found that the TMAS-TUS treatment effectively reduced amyloid plaque loads and plaque-associated neurotoxicity. Additionally, TMAS-TUS treatment ameliorated impairments in long-term memory formation and long-term potentiation. Moreover, TMAS-TUS treatment stimulated microglial proliferation and migration while enhancing the phagocytosis and clearance of Aβ. In 5xFAD mice with induced microglial exhaustion, TMAS-TUS treatment-mediated Aβ plaque reduction, synaptic rehabilitation improvement, and the increase in phospho-AKT levels were diminished. Overall, our study highlights that stimulation of hippocampal microglia by TMAS treatment can induce anti-cognitive impairment effects via PI3K-AKT signaling, providing hope for the development of new strategies for an adjuvant therapy for Alzheimer’s disease.

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Section: Aβ Phagocytosis Assays Of Bv2 Cellsmentioning

confidence: 99%

Transcranial Magneto-Acoustic Stimulation Protects Synaptic Rehabilitation from Amyloid-Beta Plaques via Regulation of Microglial Functions

Zhang,

Tan,

Zhou

et al. 2024

IJMS

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show abstract

“…In addition, Sosnik et al reported that TSSK6 is involved in IZUMO1 redistribution through the regulation of actin polymerization after the acrosomal reaction. Indeed, the involvement of F-actin via histone acetylation regulation has recently been demonstrated, being located at the apical part of the midpiece [26].…”

Section: Introductionmentioning

confidence: 99%

IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa

Gómez-Torres,

Hernández-Falcó,

López-Botella

et al. 2023

Biomedicines

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IZUMO1 is an acrosome transmembrane protein implicated in the adhesion and fusion of gametes. This study aims to describe the distribution of IZUMO1 in human sperm under different physiological conditions: before capacitation (NCS), at one-hour capacitation (CS1), after a hyaluronic acid (HA) selection test (mature, MS1 and immature, IS1), and induced acrosome reaction from one-hour-capacitated sperm (ARS1). The data obtained in NCS, CS1, and MS1 significantly highlight dotted fluorescence in the acrosomal region (P1) as the major staining pattern (~70%). Moreover, we describe a new distribution pattern (P2) with a dotted acrosomal region and a labelled equatorial region that significantly increases in HA-bound spermatozoa, suggesting the onset of the migration of IZUMO1. In contrast, unbound spermatozoa presented an increase in P3 (equatorial region labelled) and P4 (not labelled). Finally, costaining to observe IZUMO1 distribution and acrosome status was performed in ARS1. Interestingly, we reported a variety of combinations between the IZUMO1 staining patterns and the acrosomal stages. In conclusion, these data show as a novelty the diffusion of the IZUMO1 protein during different physiological conditions that could contribute to the improvement in sperm selection techniques.

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The Dual Roles of Lamin A/C in Mechanosensation to Compressive Force

Groeger,

Wang,

Ruf

et al. 2023

Preprint

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Cellular mechanosensation is a complex physiological process coupling alterations in the external environment and cellular behaviors. Over the past decade, the role of the nucleus in mechanosensation has gained increasing attention. Our research found that lamin A/C, a component of the nuclear envelope, plays a dual role in the mechanosensation of macrophages in response to compressive force. Our findings indicated that hydrostatic compressive force downregulated lamin A/C protein via the cytoskeleton. Consequently, this lamin A/C deficiency enhanced compressive-force-induced inflammatory cytokines secretion and proliferative impairment. Unexpectedly, lamin A deficiency also inhibits compressive force-induced DNA damage and interferon regulatory factor 4 (IRF4) up-regulation. Our findings suggest that lamin A/C is involved in multiple mechanosensation mechanisms. Mechanistically, lamin A/C deficiency augments nuclear permeability, facilitates the activation of yes-associated protein 1 (YAP1) and promotes force-induced nuclear translocation of YAP1. These mechanisms have been validated to favor mechanosensation. Conversely, we also found that lamin A/C deficiency led to detachment of components of linker of nucleoskeleton and cytoskeleton (LINC) complex, which impeded intracellular mechanotransmission. In summary, lamin A/C can promote some responses of macrophages to mechanical compression but inhibits others. It is involved in two distinct mechanisms: enhancing nuclear permeability to transcription factors and impairing mechanotransmission by disrupting the LINC complex's connection to the nucleus.

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Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa

Cited by 3 publications

References 47 publications

Transcranial Magneto-Acoustic Stimulation Protects Synaptic Rehabilitation from Amyloid-Beta Plaques via Regulation of Microglial Functions

Transcranial Magneto-Acoustic Stimulation Protects Synaptic Rehabilitation from Amyloid-Beta Plaques via Regulation of Microglial Functions

IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa

The Dual Roles of Lamin A/C in Mechanosensation to Compressive Force

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