Cyto- And Histochemistry of the Liver

Wachstein, M.

doi:10.1016/b978-1-4832-2824-2.50010-8

Cited by 34 publications

(14 citation statements)

References 136 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cytochrome oxidase gradient across the liver lobule: According to Burstone (3), Mattenheimer et al (9) and Wachstein (13), cytochrome oxidase activity is stronger in the periportal areas of the lobule than in the central ones in rat, hamster and some other species. As these findings remain qualitative, some quantitative analysis seems necessary to get a close insight into the activity distribution in the liver lobule.…”

Section: Discussionmentioning

confidence: 99%

Cobalt acetylacetonate-diaminobenzidine reaction for microspectrophotometry of cytochrome oxidase.

Hiraoka

Hirai

Ueda

1986

Acta Histochem. Cytochem

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Cobalt acetylacetonate-diaminobenzidine reaction for microspectrophotometry of cytochrome oxidase.

Hiraoka

Hirai

Ueda

1986

Acta Histochem. Cytochem

View full text Add to dashboard Cite

“…This is true with regard to cell organelle number and size (Loud, 1968;Uchiyama and Asari, 1984), ploidy (Chen et al, 1995), pattern of enzymatic activity (Rabes, 1978;Wachstein, 1963), autonomous innervation, number of nonparenchymatous cells, concentration of hormones (Balks and Jungermann, 1984;Jungermann and Katz, 1989;Katz, 1989), substrates and their metabolites Address correspondence to: Silke Burkhardt, Department of Product Safety, Regulations, Experimental Toxicology and Ecology, BASF AG, GV/T -Z 470, 67056 Ludwigshafen, Germany; e-mail: silke.burkhardt@ basf-ag.de (Thurman et al, 1989), and the partial O 2 pressure (Rappaport et al, 1954;Rappaport, 1963). These differences might influence the metabolism of the administered compound, and as further consequence, cell turnover.…”

Section: Introductionmentioning

confidence: 99%

Zonal Distribution of Cell Proliferation in the Liver of Untreated B6C3F1 and C57BL Mice

et al. 2004

View full text Add to dashboard Cite

To determine the lobular distribution of hepatocellular proliferation, S-phase response was measured in untreated adult male B6C3F1 and C57BL mice at ages 11, 14, and 23 weeks. The percentage of cells in S-phase (labeling index, LI) was evaluated using immunohistochemical detection of 5-bromo-2 -deoxyuridine (BrdU). The BrdU was delivered either by a single ip injection 2 hr prior to sacrifice or via an osmotic minipump implanted subcutaneously for 3 or 7 days. Mitotic and apoptotic indices were determined on H&E stained sections. Animals of both strains and all ages revealed highest LI in the intermediate compartment of the liver acinus (zone 2) irrespective of the length of BrdU application. Cell proliferation in this zone was at all time points significantly higher than elsewhere in the liver. The mitotic index confirmed the data obtained by the S-phase response study. Apoptosis was rarely observed. With regard to rodent data in chemical carcinogenesis and the way they should be evaluated, this study proved that the acinar distribution of proliferating cells in liver of mice is different from that in rats, since, according to the literature, rats reveal highest cell proliferative activity in the periportal zone.

show abstract

“…Alkaline phosphatase staining is generally located on the sinusoidal borders of the hepatocytes in the region of the terminal hepatic venules in both birds and mammals, however, in the calf and rat livers, increased staining occurs in the hepatocytes in the areas of the terminal portal venules (Thorpe and Ford, 1968;Manns and Mortimer, 1969). NAD-D and malate dehydrogenase stainings are diffuse throughout the hepatic parenchyma in both birds and mammals, however, in the rat liver there is increased staining by both of these reactions in the areas of the terminal hepatic venules (Wachstein, 1959(Wachstein, , 1963Manns and Mortimer, 1969). Isocitrate dehydrogenase staining is diffuse in both avian and mammalian livers, however, Manns and Mortimer (1969) demonstrated a slight increase in activity of this enzyme in the hepatocytes in the regions of the terminal hepatic venules.…”

Section: Adenosinementioning

confidence: 87%

“…The histochemical reactions which occur similarly in both groups are: glucose-6-phosphatase and succinate dehydrogenase located in the hepatocytes in the regions of the terminal portal venules (Wachstein, 1963;Thorpe and Ford, 1968;Manns and Mortimer, 1969); NADP-D in the hepatocytes in the regions of the terminal hepatic venules (Manns and Mortimer, 1969); adenosine triphosphatase on the canalicular borders and a widening of the canaliculi in the portal regions (Wachstein, 1963;Manns and Mortimer, 1969); and non-specific esterase which exhibits a diffuse reaction throughout the liver lobule except for a slight increase in staining in the hepatocytes in the regions of both the terminal hepatic and portal venules (Wachstein, 1963).…”

Section: Adenosinementioning

confidence: 97%

A Histochemical Study of the Avian Liver

Ratzlafe

Tyler

1973

Poultry Science

View full text Add to dashboard Cite

The enzymatic histochemistry of the avian hver is described and compared with the reported results on the mammalian Uver. The following results were obtained: Acid phosphatase staining was located in the Kupffer cells lining the sinusoids; some localization occurred on the sinusoidal margins of the hepatic cells in the region of the terminal hepatic venules. Adenosine triphosphatase staining was located on the canalicular boundaries of the hepatic cells. Non-specific esterase staining was generally diffuse but some localization occurred in the hepatic cells adjacent to both the terminal hepatic and portal venules. The highest concentration of alkaline phosphatase and nicotinamide-adenine dinucleotide phosphate diaphorase stains were located in the hepatic cells in the regions of the terminal hepatic venules. Glucose-6-phosphatase and succinate dehydrogenase localizations were greatest in the hepatic cells in the vicinity of the portal canals. Malate dehydrogenase, isocitrate dehydrogenase and nicotinamide-adenine dinucleotide diaphorase exhibited diffuse reactions throughout the hepatic parenchyma. ABSTRACTHearts of embryonic turkeys, 10 days and older, and young poults, 1 day, 1 week, and 6 weeks old, were fixed in either cold (4 C C.) 10% formalin or Rossman's mixture. Adjacent sections, 7^. in thickness, were stained with: (a) the periodic acid-Schiff technic of McManus with hematoxylin counterstain and salivary amylase controls, (b) hematoxylin and eosin, (c) Mallory's triple stain, and (d) Movat's pentachrome stain.The first histologic indication of conduction cells was seen in the hearts of 15-day embryos. These cells were seen both in the subendocardial region and perivascularly, especially in relation to the larger vessels present in the myocardium. Results indicate that conduction cells: (1) arise de novo, (2) occur extensively throughout the turkey heart, (3) are void of stainable glycogen, (4) are surrounded by a reticular connective tissue sheath, (5) possess striations, (6) are often binucleate, (7) are appreciably larger than the usual myocardial cells, and (8) usually are joined together to form fibers with no apparent intercalated discs separating the individual cells as in true myocardial fibers. by guest on May 14, 2015 http://ps.oxfordjournals.org/ Downloaded from

show abstract

Cyto- And Histochemistry of the Liver

Cited by 34 publications

References 136 publications

Cobalt acetylacetonate-diaminobenzidine reaction for microspectrophotometry of cytochrome oxidase.

Cobalt acetylacetonate-diaminobenzidine reaction for microspectrophotometry of cytochrome oxidase.

Zonal Distribution of Cell Proliferation in the Liver of Untreated B6C3F1 and C57BL Mice

A Histochemical Study of the Avian Liver

Contact Info

Product

Resources

About