Cyto- and genotoxicity of a vanadyl(IV) complex with oxodiacetate in human colon adenocarcinoma (Caco-2) cells: potential use in cancer therapy

Virgilio, Ana Laura Di; Rivadeneira, Josefina; Muglia, Cecilia Isabel; Reigosa, Miguel A.; Butenko, Nataliya; Cavaco, Isabel; Etcheverry, Susana B.

doi:10.1007/s10534-011-9474-x

Cited by 13 publications

(7 citation statements)

References 57 publications

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“…These results correlated with those of the viability study as previously reported for other vanadium derivatives. [24][25][26]…”

Section: Dalton Transactions Papermentioning

confidence: 99%

“…This action agreed with the effect on plasmidic DNA causing single and double strand cleavage. 26 We have also studied the genotoxicity of hydroxylamido-amino acid complexes of oxovanadium(V) in osteoblast-like cell lines, showing an effect dependent on the cell line. 34 On the other hand, in vivo experiments showed the genotoxicity of tetravalent vanadium.…”

Section: Papermentioning

confidence: 99%

“…Results previously reported by our group have also shown that ROS formation was also dependent on the cellular type. 24,26 The increase in ROS levels has been associated with cell death or membrane injury. It has been reported that high levels of ROS can induce apoptosis by activating either the ER stress-mediated apoptotic pathway or the mitochondrial-mediated apoptotic pathway or both.…”

Section: Mechanism Of Actionmentioning

confidence: 99%

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Antitumor properties of a vanadyl(iv) complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 in a human osteosarcoma model: the role of oxidative stress and apoptosis

et al. 2013

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Flavonoids, a polyphenolic compound family, and the vanadium compounds have interesting biological, pharmacological, and medicinal properties. We report herein the antitumor actions of the complex [VO(chrysin)2EtOH]2 (VOchrys) on the MG-63 human osteosarcoma cell line. Oxovanadium(IV), chrysin and VOchrys caused a concentration-dependent inhibition of cell viability. The complex was the strongest antiproliferative agent (p < 0.05). Cytotoxicity and genotoxicity studies also showed a concentration effect. Reactive oxygen species (ROS) and the alterations in the GSH/GSSG ratio underlie the main mechanisms of action of VOchrys. Additions of ROS scavengers (vitamin C plus vitamin E) or GSH to the viability experiments demonstrated beneficial effects (p < 0.01). Besides, the complex triggered apoptosis, disruption of the mitochondria membrane potential (MMP), increased levels of caspase 3 and DNA fragmentation measured by the sub-G1 peak in cell cycle arrest experiments (p < 0.01). Collectively, VOchrys is a cell death modulator and a promissory complex to be used in cancer treatments.

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“…These results correlated with those of the viability study as previously reported for other vanadium derivatives. [24][25][26]…”

Section: Dalton Transactions Papermentioning

confidence: 99%

Section: Papermentioning

confidence: 99%

Section: Mechanism Of Actionmentioning

confidence: 99%

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Antitumor properties of a vanadyl(iv) complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 in a human osteosarcoma model: the role of oxidative stress and apoptosis

et al. 2013

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“…It has been reported that these compounds, depending on the cellular line, may promote the generation of the reactive oxygen species (ROS) mainly in the mitochondria (Leon et al 2012a, 2014b; Rivadeneira et al 2009; Di Virgilio et al 2011) leading to a decrease of glutathione (GSH) concentration. GSH is one of the mayor reducing agents responsible for maintaining the cellular redox status through the balance of the couple glutathione/glutathione disulphide (GSH/GSSG).…”

Section: Resultsmentioning

confidence: 99%

Characterization and cytotoxic effect of aqua-(2,2′,2′′-nitrilotriacetato)-oxo-vanadium salts on human osteosarcoma cells

et al. 2017

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The use of protonated N-heterocyclic compound, i.e. 2,2′-bipyridinium cation, [bpyH+], enabled to obtain the new nitrilotriacetate oxidovanadium(IV) salt of the stoichiometry [bpyH][VO(nta)(H2O)]H2O. The X-ray measurements have revealed that the compound comprises the discrete mononuclear [VO(nta)(H2O)]− coordination ion that can be rarely found among other known compounds containing nitrilotriacetate oxidovanadium(IV) moieties. The antitumor activity of [bpyH][VO(nta)(H2O)]H2O and its phenanthroline analogue, [phenH][VO(nta)(H2O)](H2O)0.5, towards human osteosarcoma cell lines (MG-63 and HOS) has been assessed (the LDH and BrdU tests) and referred to cis-Pt(NH3)2Cl2 (used as a positive control). The compounds exert a stronger cytotoxic effect on MG-63 and HOS cells than in untransformed human osteoblast cell line. Thus, the [VO(nta)(H2O)]− containing coordination compounds can be considered as possible antitumor agents in the osteosarcoma model of bone-related cells in culture.Electronic supplementary materialThe online version of this article (doi:10.1007/s10534-017-0001-6) contains supplementary material, which is available to authorized users.

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“…This action agreed with the effect of this complex on plasmidic DNA causing single and double strand cleavage. 61 On the other hand, genotoxicity of tetravalent vanadium was also evaluated in vivo. The subacute oral exposure to vanadyl sulphate (VOSO 4 ) in male CD1 mice caused the increase in the incidence of micronucleated blood reticulocytes and bone marrow polychromatic erythrocytes, in addition to DNA lesions detectable by the comet assay.…”

Section: Discussionmentioning

confidence: 99%

Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model

et al. 2012

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Oxovanadium(V) complexes [VO(NH(2)O)(2)(val)] and [VO(NH(2)O)(2)(met)] caused inhibition of cell proliferation in two osteoblast cell lines, MC3T3-E1 and UMR106, as well as the viability of zebrafish eggs. In MC3T3-E1, both compounds inhibited cell proliferation (up to ca. 40% at 25 μM [VO(NH(2)O)(2)(val)] and 25% at 25 μM [VO(NH(2)O)(2)(met)]). This effect occurs in a dose response manner from 2.5 μM (p < 0.01) with a more deleterious action of [VO(NH(2)O)(2)(met)]. In UMR106 tumoral cells, [VO(NH(2)O)(2)(val)] inhibited cell proliferation up to 75% from 25 μM while [VO(NH(2)O)(2)(met)] behaved as an inhibitory agent in the whole range of concentrations (p < 0.01). Similar toxic effects were obtained from morphological studies in cell cultures. Moreover, the IC(50) values for both complexes in culture studies correlated with the IC(50) values obtained with an in vivo model of toxicity (FET test). Besides, the cytotoxicity evaluation in cell culture showed a decrease in mitochondrial activity which was stronger for [VO(NH(2)O)(2)(met)] than for [VO(NH(2)O)(2)(val)] (44% vs. 58% at 25 μM) in both cell lines (p < 0.001). Genotoxicity assessed by micronuclei induction also showed a stronger effect of [VO(NH(2)O)(2)(met)] in both cell lines. Besides, [VO(NH(2)O)(2)(val)] caused DNA damage determined by comet formation in MC3T3-E1 cells in the range of 2.5-25 μM, while this effect could not be observed in the osteosarcoma cells. On the other hand, [VO(NH(2)O)(2)(val)] enhanced ROS levels over basal up to 225% and 170% at 100 μM in MC3T3-E1 and UMR106 cells, respectively (p < 0.01). For [VO(NH(2)O)(2)(met)] a similar situation was observed, suggesting an important role for oxidative stress in the toxicity mechanism of action. Although both complexes showed interesting results that would deserve further drug development [VO(NH(2)O)(2)(val)] was more stable than [VO(NH(2)O)(2)(met)] in the solid state. Therefore, we consider that [VO(NH(2)O)(2)(val)] is a good candidate to be tested in in vivo models as a potential antitumoral agent.

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Cyto- and genotoxicity of a vanadyl(IV) complex with oxodiacetate in human colon adenocarcinoma (Caco-2) cells: potential use in cancer therapy

Cited by 13 publications

References 57 publications

Antitumor properties of a vanadyl(iv) complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 in a human osteosarcoma model: the role of oxidative stress and apoptosis

Antitumor properties of a vanadyl(iv) complex with the flavonoid chrysin [VO(chrysin)2EtOH]2 in a human osteosarcoma model: the role of oxidative stress and apoptosis

Characterization and cytotoxic effect of aqua-(2,2′,2′′-nitrilotriacetato)-oxo-vanadium salts on human osteosarcoma cells

Hydroxylamido–amino acid complexes of oxovanadium(v). Toxicological study in cell culture and in a zebrafish model

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