Cyto-3D-print to attach mitotic cells

Abstract

The Cyto-3D-print is an adapter that adds cytospin capability to a standard centrifuge. Like standard cytospinning, Cyto-3D-print increases the surface attachment of mitotic cells while giving a higher degree of adaptability to other slide chambers than available commercial devices. The use of Cyto-3D-print is cost effective, safe, and applicable to many slide designs. It is durable enough for repeated use and made of biodegradable materials for environment-friendly disposal.Electronic supplementary materialThe online version of this article (doi:10.1007/s10616-015-9917-2) contains supplementary material, which is available to authorized users.

Glioblastoma induces the recruitment and differentiation of hybrid neutrophils from skull bone marrow

“…Isolated patient TANs/PBNs and murine BMNs were resuspended in supplemented RPMI and 25,000 cells were seeded per well into 8-well Nunc Lab-Tek chamber slides (Thermo Fisher). Slides were placed into a custom 3D-printed adapter (Castroagudin et al, 2016) and spun at 130 rcf for 5 minutes in a standard Eppendorf 5415 centrifuge. Wells were removed and samples were allowed to air-dry completely.…”