Cytisus villosus from Northeastern Algeria is nodulated by genetically diverse Bradyrhizobium strains

Ahnia, Hadjira; Boulila, Farida; Boulila, Abdelghani; Boucheffa, Karima; Durán, David; Bourebaba, Yasmina; Salmi, Adouda; Imperial, Juan; Ruiz-Argüeso, Tomás; Rey, Luís

doi:10.1007/s10482-014-0173-9

Cited by 15 publications

(6 citation statements)

References 42 publications

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“…The NI values among the strains from our study and related type strains ranged from 99.4 to 99.9 % (). The results agree with several reports that the 16S rRNA sequences are highly conserved in the genus Bradyrhizobium , with many species sharing more than 99 % NI, not allowing proper evolutionary information [7, 12, 13, 34].…”

Section: Phylogenysupporting

confidence: 90%

Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., isolated from nodules of legumes indigenous to Western Australia

Helene

Klepa

O’Hara

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The genus Bradyrhizobium is considered as the probable ancestor lineage of all rhizobia, broadly spread in a variety of ecosystems and with remarkable diversity. A polyphasic study was performed to characterize and clarify the taxonomic position of eight bradyrhizobial strains isolated from indigenous legumes to Western Australia. As expected for the genus, the 16S rRNA gene sequences were highly conserved, but the results of multilocus sequence analysis with four housekeeping genes (dnaK, glnII, gyrB and recA) confirmed three new distinct clades including the following strains: (1) WSM 1744T, WSM 1736 and WSM 1737; (2) WSM 1791T and WSM 1742; and (3) WSM 1741T, WSM 1735 and WSM 1790. The highest ANI values of the three groups in relation to the closest type strains were 92.4, 92.3 and 93.3 %, respectively, below the threshold of species circumscription. The digital DNA–DNA hybridization analysis also confirmed new species descriptions, with less than 52 % relatedness with the closest type strains. The phylogeny of the symbiotic gene nodC clustered the eight strains into the symbiovar retamae, together with seven Bradyrhizobium type strains, sharing from 94.2–98.1 % nucleotide identity (NI), and less than 88.7 % NI with other related strains and symbiovars. Morpho-physiological, phylogenetics, genomic and symbiotic traits were determined for the new groups and our data support the description of three new species, Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., with WSM 1744T (=CNPSo 4013T=LMG 31646T), WSM 1791T (=CNPSo 4014T=LMG 31647T) and WSM 1741T (=CNPSo 4020T=LMG 31651T) designated as type strains, respectively.

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Section: Phylogenysupporting

confidence: 90%

Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., isolated from nodules of legumes indigenous to Western Australia

Helene

Klepa

O’Hara

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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“…(i) Symbiovar genistearum was described by Vinuesa et al (45) and includes many strains nodulating a large number of plants from the tribe Genisteae, including the genera Lupinus, Cytisus, Retama, Chamaecytisus, and Spartium, growing in Africa, Europe, and America (12,33,39,40,45). The majority of L. micranthus-associated isolates related to B. lupini (group I), and those similar to B. cytisi/B.…”

Section: Discussionmentioning

confidence: 99%

“…lupini and B. cytisi/B. rifense lineages and to symbiovar genistearum (16,33,52). Importantly, these microorganisms may be dominant in neutral or acidic soils.…”

Section: Discussionmentioning

confidence: 99%

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Diverse Bacteria Affiliated with the Genera Microvirga, Phyllobacterium, and Bradyrhizobium Nodulate Lupinus micranthus Growing in Soils of Northern Tunisia

Msaddak

Durán

Rejili

et al. 2017

Appl Environ Microbiol

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The genetic diversity of bacterial populations nodulating Lupinus micranthus in five geographical sites from northern Tunisia was examined. Phylogenetic analyses of 50 isolates based on partial sequences of recA and gyrB grouped strains into seven clusters, five of which belong to the genus Bradyrhizobium (28 isolates), one to Phyllobacterium (2 isolates), and one, remarkably, to Microvirga (20 isolates). The largest Bradyrhizobium cluster (17 isolates) grouped with the B. lupini species, and the other five clusters were close to different recently defined Bradyrhizobium species. Isolates close to Microvirga were obtained from nodules of plants from four of the five sites sampled. We carried out an in-depth phylogenetic study with representatives of the seven clusters using sequences from housekeeping genes (rrs, recA, glnII, gyrB, and dnaK) and obtained consistent results. A phylogeny based on the sequence of the symbiotic gene nodC identified four groups, three formed by Bradyrhizobium isolates and one by the Microvirga and Phyllobacterium isolates. Symbiotic behaviors of the representative strains were tested, and some congruence between symbiovars and symbiotic performance was observed. These data indicate a remarkable diversity of L. micranthus root nodule symbionts in northern Tunisia, including strains from the Bradyrhizobiaceae, Methylobacteriaceae, and Phyllobacteriaceae families, in contrast with those of the rhizobial populations nodulating lupines in the Old World, including L. micranthus from other Mediterranean areas, which are nodulated mostly by Bradyrhizobium strains.IMPORTANCE Lupinus micranthus is a legume broadly distributed in the Mediterranean region and plays an important role in soil fertility and vegetation coverage by fixing nitrogen and solubilizing phosphate in semiarid areas. Direct sowing to extend the distribution of this indigenous legume can contribute to the prevention of soil erosion in pre-Saharan lands of Tunisia. However, rhizobial populations associated with L. micranthus are poorly understood. In this context, the diversity of endosymbionts of this legume was investigated. Most Lupinus species are nodulated by Bradyrhizobium strains. This work showed that about half of the isolates from northern Tunisian soils were in fact Bradyrhizobium symbionts, but the other half were found unexpectedly to be bacteria within the genera Microvirga and Phyllobacterium. These unusual endosymbionts may have a great ecological relevance. Inoculation with the appropriate selected symbiotic

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“…nov. and B. rifense sp. nov. in Morocco [39,40] and by genetically diverse Bradyrhizobium strains in Algeria belonging to B. japonicum and B. canariense and to new lineage within the Bradyrhizobium genus [41]. Fiftytwo strains isolated from root nodules of the Moroccan shrubby legume Cytisus triflorus were genetically characterized, and results showed that it is nodulated by Bradyrhizobium strains, with 99% homology with Bradyrhizobium genosp.…”

Section: The Genus Bradyrhizobium (Bradyrhizobiaceae)mentioning

confidence: 99%

Phylogenomic Review of Root Nitrogen-Fixing Symbiont Population Nodulating Northwestern African Wild Legumes

Rejili¹,

Benabderrahim²,

Mars³

2020

Nitrogen Fixation

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The present review discusses the phylogenomic diversity of root nitrogen-fixing bacteria associated to wild legumes under North African soils. The genus Ensifer is a dominant rhizobium lineage nodulating the majority of the wild legumes, followed by the genus Rhizobium and Mesorhizobium. In addition, to the known rhizobial genera, two new Microvirga and Phyllobacterium genera were described as real nodulating and nitrogen-fixing microsymbiotes from Lupinus spp. The promising rhizobia related to nitrogen fixation efficiency in association with some legumes are shared. Phylogenetic studies are contributing greatly to our knowledge of relationships on both sides of the plant-bacteria nodulation symbiosis. Multiple origins of nodulation (perhaps even within the legume family) appear likely. However, all nodulating flowering plants are more closely related than previously suspected, suggesting that the predisposition to nodulate might have arisen only once. The origins of nodulation, and the extent to which developmental programs are conserved in nodules, remain unclear, but an improved understanding of the relationships between nodulin genes is providing some clues.

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Cytisus villosus from Northeastern Algeria is nodulated by genetically diverse Bradyrhizobium strains

Cited by 15 publications

References 42 publications

Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., isolated from nodules of legumes indigenous to Western Australia

Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., isolated from nodules of legumes indigenous to Western Australia

Diverse Bacteria Affiliated with the Genera Microvirga, Phyllobacterium, and Bradyrhizobium Nodulate Lupinus micranthus Growing in Soils of Northern Tunisia

Phylogenomic Review of Root Nitrogen-Fixing Symbiont Population Nodulating Northwestern African Wild Legumes

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