Cyt1Aa protein fromBacillus thuringiensis(Berliner) serovarisraelensisis active against the Mediterranean fruit fly,Ceratitis capitata(Wiedemann)

Vidal-Quist, José Cristian; Castañera, Pedro; González‐Cabrera, Joel

doi:10.1002/ps.1965

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…The lethal concentration 50 (LC 50 ) and LC 90 value were calculated after 24 h by probit analysis [26]. A corrected mortality percentage which regarded mortality or dead larvae in the negative control was calculated using Abbott's formula [27][28][29][30]. The corrected mortality = ([% mortality in treatment -% mortality in control]/[100 -% mortality in control]) × 100.…”

Section: Larvicidal Bioassay Of Anopheles Larvaementioning

confidence: 99%

LARVICIDAL ACTIVITY OF BACILLUS THURINGIENSIS ISOLATED FROM Bt COTTON RHIZOSPHERE SOIL AGAINST ANOPHELES MOSQUITO LARVAE (CULICIDAE)

Rajendran

Nathiya

Velu

2018

Asian J Pharm Clin Res

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Objective: The objective of the study was to isolate and identify Bacillus thuringiensis (Bt) from Bt cotton rhizosphere soil and analyze its larvicidal activity against Anopheles mosquito larvae.Methods: Soil samples were collected from Bt cotton field, and B. thuringiensis was isolated and characterized by biochemical and microscopical characterization. Crystal (Cry) proteins were extracted and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and its larvicidal activity was checked by mortality analysis. Effective Bt isolates were identified by 16S rRNA sequencing.Results: A total of 24 isolates were characterized as Bacillus sp. by biochemical characterization. Further, parasporal inclusion and Cry proteins were extracted, and it was quantified by Bradford assay. The precipitated Cry proteins were analyzed by SDS-PAGE and the results have indicates Cry I protein at 100kDa, Cry3 protein at 44kDa, and cytolytic protein (Cyt) at 29kDa in most of the isolates. Larvicidal activity was checked by the 4th instar Anopheles mosquito larvae. Among the tested isolates, RBL10 and RBL20 have shown the highest percentage of larvicidal activity at 96 and 83%, respectively. Further, the two isolates RBL10 and RBL20 were identified as Bt by 16S rRNA sequencing.Conclusion: B. thuringiensis producing Cry and Cyt proteins posses potential larvicidal activity against Anopheles mosquito larvae which has biological and economic importance in mosquito control.

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Section: Larvicidal Bioassay Of Anopheles Larvaementioning

confidence: 99%

LARVICIDAL ACTIVITY OF BACILLUS THURINGIENSIS ISOLATED FROM Bt COTTON RHIZOSPHERE SOIL AGAINST ANOPHELES MOSQUITO LARVAE (CULICIDAE)

Rajendran

Nathiya

Velu

2018

Asian J Pharm Clin Res

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“…In addition to mosquitoes, black flies [23] and chironomid midges [24,25] the expanded host range of Bti includes the following species: Tabanus triceps (Diptera: Tabanidae) [26], Mexican fruit fly, Anastrepha ludens and Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae) [27,28], Tipula paludosa (Diptera: Nematocera) [29], fungus gnats, Bradysia coprophila and Bradysia impatiens (Diptera: Sciaridae) [30,31], nodule-damaging fly Rivellia angulata (Diptera: Platystomatidae) [32], pea aphid Acyrthosiphon pisum (Hemiptera: Aphidoidea) [33], potato aphid, Macrosiphum euphorbiae (Homoptera: Aphididae) [34], cotton boll weevil Anthonomus grandis (Coleoptera: Tenebrionidae) [35], leaf beetle, Chrysomela scripta (Coleoptera: Chrysomelidae) [36], fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) [37], diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae) [38], root-knot nematode, Meloidogyne incognita on barley [39] and trematode species, Schistosoma mansoni and Trichobilharzia szidati (Trematoda: Schistosomatidae) [40]. …”

Section: The Bacterium: Bacillus Thuringiensis Subsp Israelensismentioning

confidence: 99%

Bacillus thuringiensis subsp. israelensis and Its Dipteran-Specific Toxins

Ben‐Dov

2014

Toxins

187

149

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Bacillus thuringiensis subsp. israelensis (Bti) is the first Bacillus thuringiensis to be found and used as an effective biological control agent against larvae of many mosquito and black fly species around the world. Its larvicidal activity resides in four major (of 134, 128, 72 and 27 kDa) and at least two minor (of 78 and 29 kDa) polypeptides encoded respectively by cry4Aa, cry4Ba, cry11Aa, cyt1Aa, cry10Aa and cyt2Ba, all mapped on the 128 kb plasmid known as pBtoxis. These six δ-endotoxins form a complex parasporal crystalline body with remarkably high, specific and different toxicities to Aedes, Culex and Anopheles larvae. Cry toxins are composed of three domains (perforating domain I and receptor binding II and III) and create cation-selective channels, whereas Cyts are composed of one domain that acts as well as a detergent-like membrane perforator. Despite the low toxicities of Cyt1Aa and Cyt2Ba alone against exposed larvae, they are highly synergistic with the Cry toxins and hence their combinations prevent emergence of resistance in the targets. The lack of significant levels of resistance in field mosquito populations treated for decades with Bti-bioinsecticide suggests that this bacterium will be an effective biocontrol agent for years to come.

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“…The partially purified soybean meal extract unexpectedly processed DL-BApNA, indicating that it contained a measurable level of amidolytic activity (Shamsi et al, 2016). This finding suggests the presence of proteases in the soybean meal preparation, a feature that could be of benefit for the purposes of this work, given that Cry and Cyt proteins digested by insect endo-proteases may exhibit increased insecticidal activity (Vidal-Quist et al, 2010).…”

Section: Discussionmentioning

confidence: 76%

Soybean protease inhibitors increase Bacillus thuringiensis subs. israelensis toxicity against Hypothenemus hampei

et al. 2020

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Introduction. The coffee berry borer (Hypothenemus hampei Ferrari, CBB) is one of the most devastating pests on coffee plantations around the world. Although CBB is susceptible to the effect of δ–endotoxins of Bacillus thuringiensis subs. israelensis (Bti) at laboratory level, the efficacy of this control method is poor in the field, presumably due to the inactivation by digestive proteases different to those required for protoxin activation. Objective. To study whether the addition of a soybean flour extract enriched with protease inhibitors (PI), mixed with Bti crystals and spores (Bti-sc) in an artificial diet, could improve the toxicity of Bti against CBB. Materials and methods. This study was performed in San José, Costa Rica, between 2012 and 2013. A set of adult female CBB insects was exposed to a mixture containing different concentrations of a partially purified soybean meal extract with active PI and lyophilized Bti-sc, and were tested through a bioassay in artificial diet to estimate the sub-lethal concentration (LC50). The mortality results were validated by observing the dissected midgut, whose ultrastructure was analyzed by transmission electron microscopy. Results. The soybean extracts partially degraded the Bti-sc complex, it reduced its LC50 by almost four times (from 1.135 to 0.315 µg µl-1) and enhanced CBB mortality in a concentration-dependent manner. Histological analyses of the midgut confirmed this synergistic effect, since severe epithelial damage to the intestinal epithelium of CBB exposed to Bti-sc + PI was visualized compared to Bti-sc alone. Conclusions. The combination of a soybean extract enriched in PI and Bti-sc enhanced the mortality effect over CBB, which was confirmed by the midgut collapse. Soybean flour is a cost-effective supplement that could increase Bti effectiveness against CBB and delay the appearance of biological resistance.

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Cyt1Aa protein fromBacillus thuringiensis(Berliner) serovarisraelensisis active against the Mediterranean fruit fly,Ceratitis capitata(Wiedemann)

Abstract: The present study provides evidence for the first time of the insecticidal activity of a B. thuringiensis strain against C. capitata and identifies a single delta-endotoxin with potential for controlling this pest.

Cited by 11 publications

References 32 publications

LARVICIDAL ACTIVITY OF BACILLUS THURINGIENSIS ISOLATED FROM Bt COTTON RHIZOSPHERE SOIL AGAINST ANOPHELES MOSQUITO LARVAE (CULICIDAE)

LARVICIDAL ACTIVITY OF BACILLUS THURINGIENSIS ISOLATED FROM Bt COTTON RHIZOSPHERE SOIL AGAINST ANOPHELES MOSQUITO LARVAE (CULICIDAE)

Bacillus thuringiensis subsp. israelensis and Its Dipteran-Specific Toxins

Soybean protease inhibitors increase Bacillus thuringiensis subs. israelensis toxicity against Hypothenemus hampei

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