In recent years, several in vitro studies have revealed that some of the LAB strains and their Cell-free supernatants (CFSs) have shown antibacterial activities and promising tools to prevent or disperse the bio lm formation of dermatological pathogens. In this study, it was aimed to investigate the antibacterial effects of LAB active cultures and CFSs against P. aeruginosa and S. aureus which were related to wound infections, as well as the inhibitory effects of CFSs on the bio lm formation of pathogens and destroying bio lms. The current results indicated that some of the LAB active cultures and CFSs have shown antibacterial properties against pathogens; furthermore, it was determined that some of the CFSs have shown powerful inhibitory effects on bio lm formation and destroying bio lms. All data together signify a great ability of the applications of CFSs as antibacterial and bio lm removal compounds that could be very suitable to control wound infections caused by these pathogens.