Cystic Echinococcosis in Central Iran: G1 and G6 Genotypes in Patients

Kargar, Saeed; Dalimi, Abdolhossein; Eslami, Gilda; Hajimohammadi, Bahador; Shirinzadeh, Ahmad; Amouei, Abdolhamid; Zare, Mohammad; Jafari-Nedooshan, Jama; Tafti, Mahmood Akhavan; Ahmadian, Salman; Hosseini, Saeedeh Sadat; Askari, Vahideh; Sheykhzadegan, Maryam; Ghoshouni, Hamed; Ranjbar, Mohammad Javad; Boozhmehrani, Mohammad Javad; Vakili, Mahmood

doi:10.1089/sur.2022.022

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…Furthermore, data from twin studies have revealed higher concordance rates among monozygotic twins (33%) compared to dizygotic twins (3%), indicating a more prominent role for heritability in the development of CTEV [ 13 ]. Presently, only one genome-wide association study (GWAS) has been conducted on CTEV, which identified genetic variants in the NCOR2 and ZNF664 genes as being associated with the pathogenesis of CTEV in non-Hispanic and European descent individuals with CTEV [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

A thorough analysis of data on the correlation between COL9A1 polymorphisms and the susceptibility to congenital talipes equinovarus: a meta-analysis

Golshan-Tafti,

Dastgheib,

Alijanpour

et al. 2024

J Orthop Surg Res

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Background Congenital talipes equinovarus (CTEV) is a prevalent pediatric deformity with a multifactorial etiology. The objective of this meta-analysis was to explore the association between genetic variations in COL9A1 and the susceptibility to CTEV. Methods A comprehensive analysis of pertinent literature released before November 15, 2023, in electronic bibliographic databases was carried out. The importance of the connection was clarified through odds ratios (ORs) with 95% confidence intervals (CIs), utilizing random or fixed-effects models depending on study heterogeneity. Statistical analysis was executed using Comprehensive Meta-Analysis software (Version 4.0). Results A total of eight case-control studies involving 833 CTEV patients and 1280 healthy individuals were included in the analysis. Among these, four studies investigated the rs1135056 variant, encompassing 432 CTEV cases and 603 controls; two studies examined the rs35470562 variant, with 189 CTEV cases and 378 controls; and two studies explored the rs592121 variant, including 212 CTEV cases and 299 controls. The results revealed a significant association between the rs1135056 and rs35470562 polymorphisms in the COL9A1 gene, suggesting an increased risk of CTEV in the overall population. Conversely, no such association was found for the rs592121 variant. Conclusion Our findings reveal a substantial association between the genetic variants COL9A1 rs1135056 and rs35470562 and susceptibility to CTEV. Conversely, the variant rs592121 did not exhibit any corresponding link. However, the limitations imposed by the small study population have compromised the statistical reliability and generalizability of the results.

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Section: Introductionmentioning

confidence: 99%

A thorough analysis of data on the correlation between COL9A1 polymorphisms and the susceptibility to congenital talipes equinovarus: a meta-analysis

Golshan-Tafti,

Dastgheib,

Alijanpour

et al. 2024

J Orthop Surg Res

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“…Considerable molecular studies on CE have been carried out in Iran during recent years [6,[10][11][12][13][14][15] . Based on the previous studies, E. granulosus s.s. and E. canadensis have been considered prevalent genotypes in different parts of Iran [6,12,16,17] .…”

Section: Introductionmentioning

confidence: 99%

Comparison of Isoenzyme Pattern of Echinococcus granulosus sensu stricto (G1-G3) and E. canadensis (G6/G7) Protoscoleces

Dousti,

Sadjjadi,

Solgi

et al. 2023

ibj

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Background: Different genotypes of Echinococcus granulosus s.l. infect humans and ungulate animals, causing cystic echinococcosis. Simultaneous isoenzyme, as well as molecular characterizations of this parasite, has not yet been investigated in Iran. The present study aimed to evaluate the isoenzyme pattern of the E. granulosus s.s. and E. canadensis genotypes in Iran. Methods: A total of 32 (8 humans and 24 animals) cystic echinococcosis cysts were isolated from Shiraz, Tehran, Ilam, and Birjand from May 2018 to December 2020. The DNAs were extracted and their genotypes were determined by molecular methods. Enzymes were extracted from the cysts and subjected to polyacrylamide gel electrophoresis. The activities of G6PD, MDH, ME, NH1, and ICD were examined in the cyst samples using isoenzyme method and compared it with the genotyping findings. Results: DNA sequence analysis of the samples showed that the specimens contained 75% E. granulosus s.s. (G1) and 25% E. canadensis (G6) genotypes. The isoenzyme pattern of ICD in both genotypes produced a six-band pattern with different relative factors. The G6PD also produced two bands with different relative migrations in both genotypes. The MDH and NH1 systems revealed a two-band pattern, while only one band was generated in the ME enzyme in the E. granulosus s.s. genotype. In the E. canadensis, the MDH and NH1 enzymes showed one band, and the ME enzyme represented a two-band pattern. Conclusion: Our findings suggest that E. granulosus s.s. and E. canadensis genotypes have entirely different isoenzyme patterns for NH1, G6PD, MDH, and ME.

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