Cysteine-rich secretory protein 3 inhibits hepatitis C virus at the initial phase of infection

Lee, Uk; Nam, Young Ran; Ye, Jung Sook; Lee, Kyung Jin; Kim, Nari; Joo, Chul Hyun

doi:10.1016/j.bbrc.2014.06.106

Cited by 4 publications

(7 citation statements)

References 26 publications

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“…We found that the expression of CRISP3 in the bulbourethral glands of adult and 3-month-old male pigs was higher than that in the other tissues. It was previously found that CRISP3 has two isoforms in human and horse semen [23,28,30], 29 kDa and 31 kDa, respectively, depending on its glycosylation status [7]. The present study revealed two forms of CRISP3 in the epididymis, seminal vesicles, and prostate glands.…”

Section: Discussionsupporting

confidence: 66%

“…Proteins in semen are assembled or bound to the surface of the sperm during sperm formation, maturation, or fertilization and play a role in the fertilization process. It has been reported that CRISP3 is expressed in the testis and epididymis of humans and through the accessory gonads of mice, but not in the epididymis of rats [7,23]. In this study, the reproductive organs of male and female sows of different ages were selected to explore the distribution of CRISP3 in the reproductive systems of male and female sows at both the gene and protein levels.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that human CRISP3 is not involved in gamete fusion [22]. The CRISP3 gene knockout mice showed that, compared to normal mice, there was no significant difference in the fertilization rate of the knockout mice, but the blastocyst rate and the number of pups were significantly reduced [17] CRISP3 protein is involved in the immune response and has a defensive role [23], which can inhibit the binding of sperm to polymorphonuclear neutrophils and prevent sperm from being cleared in the female reproductive tract [24]. We analyzed the differentially expressed proteins in the sperm of boars with high and low fertility using iTRAQ technology [25] and found that CRISP3 was highly expressed in the semen of boars with high fertility.…”

Section: Introductionmentioning

confidence: 99%

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Semen Protein CRISP3 Promotes Reproductive Performance of Boars through Immunomodulation

Bu,

Wang,

et al. 2024

IJMS

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Semen proteins play an important role in male reproductive performance and sperm fertilization ability and can be used as potential biomarkers to evaluate male fertility. The role of cysteine-rich secretory protein 3 (CRISP3) in male reproduction remains unknown. This study aimed to investigate the role of CRISP3 in the reproductive performance of boars. Our results showed that the CRISP3 protein content was significantly and positively correlated with boar fertility, sow delivery rate, and litter size. CRISP3 is highly expressed in the bulbourethral gland of adult boars and is enriched in the seminal plasma. It is localized in the post-acrosomal region of the sperm head and migrates to the anterior end of the tail after capacitation. The CRISP3 recombinant protein did not affect sperm motility and cleavage rate, but it significantly downregulated the mRNA expression of inflammatory factors IL-α, IL-1β, and IL-6 and the protein expression of IL-α and IL-6 in lipopolysaccharide (LPS)-induced RAW264.7 cells, indicating that CRISP3 has an immunomodulatory function. In conclusion, our study suggests that semen CRISP3 protein levels positively correlate with reproductive performance, which may be achieved by regulating immune responses in the female reproductive tract.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Semen Protein CRISP3 Promotes Reproductive Performance of Boars through Immunomodulation

Bu,

Wang,

et al. 2024

IJMS

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“…According to research, we can detect high levels of CRISP3 messenger RNA (mRNA) in human pancreas, salivary glands, and prostate, and we can also detect CRISP3 protein in human body fluids, like blood and saliva (Bjartell et al, ). CRISP3 possesses an inhibitory effect upon hepatitis C virus during the early infection (Lee et al, ). Furthermore, CRISP3 is likely to participate in the carcinogenesis of oral squamous cell carcinoma (Ko et al, ) or myeloma (Leng, Miao, Huang, & Wang, ).…”

Section: Introductionmentioning

confidence: 99%

“…CRISP3 possesses an inhibitory effect upon hepatitis C virus during the early infection (Lee et al, 2014). Furthermore, CRISP3 is likely to participate in the carcinogenesis of oral squamous cell carcinoma (Ko et al, 2012) or myeloma (Leng, Miao, Huang, & Wang, 2018).…”

mentioning

confidence: 99%

Low expression of CRISP3 predicts a favorable prognosis in patients with mammary carcinoma

Wang

Sheng

Xie

et al. 2019

Journal Cellular Physiology

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The discovery of cysteine‐rich secretory protein 3 (CRISP3) has been made in human neutrophils for the first time. Cloning of the complementary DNA (cDNA) for CRISP3 was performed from a cDNA library of human bone marrow. In patients with mammary carcinoma, we found that lower expression of CRISP3 was connected to a significantly improved DFS (disease‐free survival) and OS (overall survival). Furthermore, the CRISP3 protein level was significantly associated with negative ANXA1 protein level. In addition, the heterogeneous expression of CRISP3 had been exhibited in diverse mammary carcinoma cells. A significant higher mRNA and the protein level of CRISP3 were seen in T‐47D as well as SK‐BR‐3 cells compared with those in other types of mammary carcinoma cells. Knockdown of CRISP3 in T‐47D or SK‐BR‐3 cells resulted in the weakened migration or invasion abilities. Furthermore, CRISP3 knockdown significantly inhibited the ERK1/2 MAPK signaling pathway in T‐47D or SK‐BR‐3 cells. Research results indicated that the lowering in the expression of CRISP3 is likely to serve as an unprecedented approach for the treatment of mammary carcinoma.

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Cysteine-rich secretory protein 3 plays a role in prostate cancer cell invasion and affects expression of PSA and ANXA1

et al. 2015

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Cysteine-rich secretory protein 3 (CRISP-3) is upregulated in prostate cancer as compared to the normal prostate tissue. Higher expression of CRISP-3 has been linked to poor prognosis and hence it has been thought to act as a prognostic marker for prostate cancer. It is proposed to have a role in innate immunity but its role in prostate cancer is still unknown. In order to understand its function, its expression was stably knocked down in LNCaP cells. CRISP-3 knockdown did not affect cell viability but resulted in reduced invasiveness. Global gene expression changes upon CRISP-3 knockdown were identified by microarray analysis. Microarray data were quantitatively validated by evaluating the expression of seven candidate genes in three independent stable clones. Functional annotation of the differentially expressed genes identified cell adhesion, cell motility, and ion transport to be affected among other biological processes. Prostate-specific antigen (PSA, also known as Kallikrein 3) was the top most downregulated gene whose expression was also validated at protein level. Interestingly, expression of Annexin A1 (ANXA1), a known anti-inflammatory protein, was upregulated upon CRISP-3 knockdown. Re-introduction of CRISP-3 into the knockdown clone reversed the effect on invasiveness and also led to increased PSA expression. These results suggest that overexpression of CRISP-3 in prostate tumor may maintain higher PSA expression and lower ANXA1 expression. Our data also indicate that poor prognosis associated with higher CRISP-3 expression could be due to its role in cell invasion.

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Cysteine-rich secretory protein 3 inhibits hepatitis C virus at the initial phase of infection

Cited by 4 publications

References 26 publications

Semen Protein CRISP3 Promotes Reproductive Performance of Boars through Immunomodulation

Semen Protein CRISP3 Promotes Reproductive Performance of Boars through Immunomodulation

Low expression of CRISP3 predicts a favorable prognosis in patients with mammary carcinoma

Cysteine-rich secretory protein 3 plays a role in prostate cancer cell invasion and affects expression of PSA and ANXA1

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