Cysteine-rich receptor-like kinase CRK5 as a regulator of growth, development, and ultraviolet radiation responses in Arabidopsis thaliana

Burdiak, Paweł; Rusaczonek, Anna; Witoń, Damian; Głów, Dawid; Karpiński, Stanisław

doi:10.1093/jxb/erv143

Cited by 74 publications

(63 citation statements)

References 59 publications

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“…Collectively these processes as well as senescence all involve ROS, although the spatio-temporal mechanisms of ROS production, interaction and signaling in the senescence syndrome are not well understood. A probable ROS dependent role of CRK5 in cell death and senescence is documented (Burdiak et al, 2015). Our results further support an as of yet little known role of the CRKs in senescence.…”

Section: Senescence and Abscissionsupporting

confidence: 83%

“…We detected 6 CRKs showing the characteristic abundance pattern associated with senescence. This RLK gene family consists of 44 members all in close proximity on chromosome 4 with a host of different physiological functions (Wrzaczek et al, 2010;Burdiak et al, 2015). Their extracellular regions have two conserved DUF domains each with 4 cysteine residues as potential targets for thiol redox regulation.…”

Section: Senescence and Abscissionmentioning

confidence: 99%

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Reshaping of theArabidopsis thalianaproteome landscape and co-regulation of proteins in development and immunity

Bassal

Majovsky

Thieme

et al. 2020

Preprint

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24Proteome remodeling is a fundamental adaptive response and proteins in complex and 25 functionally related proteins are often co-expressed. Using a deep sampling strategy we 26 define Arabidopsis thaliana tissue core proteomes at around 10,000 proteins per tissue 27 and absolutely quantify (copy numbers per cell) nearly 16,000 proteins throughout the 28 plant lifecycle. A proteome wide survey of global post translational modification revealed 29 amino acid exchanges pointing to potential conservation of translational infidelity in 30 eukaryotes. Correlation analysis of protein abundance uncovered potentially new tissue 31 and age specific roles of entire signaling modules regulating transcription in 32 photosynthesis, seed development and senescence and abscission. Among others, the 33 data suggest a potential function of RD26 and other NAC transcription factors in seed 34 development related to desiccation tolerance as well as a possible function of Cysteine-35 rich Receptor-like Kinases (CRKs) as ROS sensors in senescence. All of the 36 components of ribosome biogenesis factor (RBF) complexes were co-expressed tissue 37 and age specifically indicating functional promiscuity in the assembly of these little 38 described protein complexes in Arabidopsis. Treatment of seedlings with flg22 for 16 39 hours allowed us to characterize proteome architecture in basal immunity in detail. The 40 results were complemented with parallel reaction monitoring (PRM) targeted 41 proteomics, phytohormone, amino acid and transcript measurements. We obtained 42 strong evidence of suppression of jasmonate (JA) and JA-Ile levels by deconjugation 43 and hydroxylation via IAA-ALA RESISTANT3 (IAR3) and JASMONATE-INDUCED 44 OXYGENASE 2 (JOX2) under the control of JASMONATE INSENSITIVE 1 (MYC2). 45 This previously unknown regulatory switch is another part of the puzzle of the as yet 46 understudied role of JA in pattern triggered immunity. The extensive coverage of the 47 Arabidopsis proteome in various biological scenarios presents a rich resource to plant 48 biologists that we make available to the community.49 50 51 52 53 Proteome biology is receiving increasing interest in the last years but still proves difficult 54 on a genome wide scale in plants. The proteome is the most fundamental active 55 determinant of an organism's phenotype and its landscape is large, complex and 56 dynamic, entailing changes in protein abundance, interaction, post translational 57 modification (PTM) and sub-cellular localization. 58 Steady state protein abundance at a certain time point is to a considerable part 59 determined by the abundance of its transcript and the latter's translation rate. Synthesis 60 is however only half of the equation governing protein abundance, indeed Arabidopsis 61 has more than 600 F-box proteins as components of diverse E3 ubiquitin ligase 62 complexes that direct protein degradation. Newer evidence has shown that post 63 transcriptional and translational mechanisms (Ponnala et al., 2014; Merchante et al., 64 2017) and...

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Section: Senescence and Abscissionsupporting

confidence: 83%

Section: Senescence and Abscissionmentioning

confidence: 99%

Reshaping of theArabidopsis thalianaproteome landscape and co-regulation of proteins in development and immunity

Bassal

Majovsky

Thieme

et al. 2020

Preprint

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“…In addition, a remarkably low number of genes deregulated in field‐grown lsd1 , in comparison to laboratory‐grown lsd1 (Wituszyńska et al ), might imply that the LSD1 suppressive role on CD is supported by other yet undetermined regulators. Potential candidates are CYSTEINE‐RICH RECEPTOR‐LIKE KINASE (Burdiak et al ), MITOGEN ACTIVATED PROTEIN KINASE 4 (Gawroński et al , Witoń et al ) and LSD ONE LIKE 1 (Epple et al ) proteins.…”

Section: Discussionmentioning

confidence: 99%

LSD1‐, EDS1‐ and PAD4‐dependent conditional correlation among salicylic acid, hydrogen peroxide, water use efficiency and seed yield in Arabidopsis thaliana

Bernacki

Czarnocka

Rusaczonek

et al. 2019

Physiologia Plantarum

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In Arabidopsis thaliana, LESION SIMULATING DISEASE 1 (LSD1), ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) and PHYTOALEXIN DEFICIENT 4 (PAD4) proteins are regulators of cell death (CD) in response to abiotic and biotic stresses. Hormones, such as salicylic acid (SA), and reactive oxygen species, such as hydrogen peroxide (H2O2), are key signaling molecules involved in plant CD. The proposed mathematical models presented in this study suggest that LSD1, EDS1 and PAD4 together with SA and H2O2 are involved in the control of plant water use efficiency (WUE), vegetative growth and generative development. The analysis of Arabidopsis wild‐type and single mutants lsd1, eds1, and pad4, as well as double mutants eds1/lsd1 and pad4/lsd1, demonstrated the strong conditional correlation between SA/H2O2 and WUE that is dependent on LSD1, EDS1 and PAD4 proteins. Moreover, we found a strong correlation between the SA/H2O2 homeostasis of 4‐week‐old Arabidopsis leaves and a total seed yield of 9‐week‐old plants. Altogether, our results prove that SA and H2O2 are conditionally regulated by LSD1/EDS/PAD4 to govern WUE, biomass accumulation and seed yield. Conditional correlation and the proposed models presented in this study can be used as the starting points in the creation of a plant breeding algorithm that would allow to estimate the seed yield at the initial stage of plant growth, based on WUE, SA and H2O2 content.

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“…AtCRK4 , AtCRK5 , AtCRK13 , AtCRK19 , AtCRK20 , and AtCRK36 can induce HR cell death in Arabidopsis (Chen et al , 2003, 2004; Acharya et al , 2007; Ederli et al , 2011; Yeh et al , 2015; Lee et al , 2017). Moreover, the crk5 mutant plants displayed enhanced cell death in response to UV radiation (Burdiak et al , 2015). Suppression of AtCRK36 expression increased sensitivity to abscisic acid (ABA) and osmotic stress in Arabidopsis (Tanaka et al , 2012).…”

Section: Introductionmentioning

confidence: 99%

A novel cysteine‐rich receptor‐like kinase gene, TaCRK2, contributes to leaf rust resistance in wheat

Sun

Liu

et al. 2020

Molecular Plant Pathology

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This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. AbstractLeaf rust, caused by Puccinia triticina, is one of the most destructive fungal diseases in wheat production worldwide. The hypersensitive reaction (HR) is an important defence response against P. triticina infection. In this study, the physiological races 165 and 260 of P. triticina were combined with a line derived from the bread wheat cultivar Thatcher with the leaf rust resistance locus Lr26 to form compatible and incompatible combinations, respectively. Based on an RNA-Seq database of the interaction systems, a new wheat cysteine-rich receptor-like kinase gene, TaCRK2, is specifically induced and up-regulated in the incompatible combination. We identified that TaCRK2 was regulated in a Ca 2+ -dependent manner. Knockdown of TaCRK2 by virus-induced gene silencing and RNAi leads to a dramatic increase in HR area and the number of haustorial mother cells at the single infection site. In addition, urediniospores, a P. triticina-specific pathogenic marker in compatible combinations, were observed on leaf surfaces of silenced plants at approximately 15 days after inoculation in the incompatible combination. Moreover, transcription levels of TaPR1, TaPR2, and TaPR5 were obviously reduced in TaCRK2-silenced plants. TaCRK2 overexpression in Nicotiana benthamiana induced strong HR-like cell death. Finally, transient expression of green fluorescent protein fused with TaCRK2 in N. benthamiana indicated that TaCRK2 localizes in the endoplasmic reticulum. Thus, TaCRK2 plays an important role in the resistance to P. triticina infection and has a positive regulation effect on the HR cell death process induced by P. triticina. K E Y W O R D S cysteine-rich receptor-like protein kinase, hypersensitive reaction, Puccinia triticina, VIGS, wheat | 733 GU et al.

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Cysteine-rich receptor-like kinase CRK5 as a regulator of growth, development, and ultraviolet radiation responses in Arabidopsis thaliana

Abstract: HighlightThe functional characterization of Arabidopsis thaliana crk5 mutant and complementation lines reveals an essential regulatory role of this gene in ROS homeostasis, which affects senescence and abiotic stress response.

Cited by 74 publications

References 59 publications

Reshaping of theArabidopsis thalianaproteome landscape and co-regulation of proteins in development and immunity

Reshaping of theArabidopsis thalianaproteome landscape and co-regulation of proteins in development and immunity

LSD1‐, EDS1‐ and PAD4‐dependent conditional correlation among salicylic acid, hydrogen peroxide, water use efficiency and seed yield in Arabidopsis thaliana

A novel cysteine‐rich receptor‐like kinase gene, TaCRK2, contributes to leaf rust resistance in wheat

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