Cysteine protease is a major exotoxin of pathogenic luminous<i>Vibrio harveyi</i>in the tiger prawn,<i>Penaeus</i>monodon

Liu, P.‐C.; Lee, K.‐K.

doi:10.1046/j.1365-2672.1999.00555.x

Cited by 60 publications

(34 citation statements)

References 22 publications

(29 reference statements)

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“…1997. Proteases have also been implicated a s virulence factors in various Vibrio strains pathogenic to fish (Norqvist et al 1990) or shrimp (Lee et al 1997, Liu & Lee 1999). The toxic activities we describe here seem to be specific, as no cytopathic effect of Vibrio strains pathogenic to shrimp was noted with the fish cell line tested.…”

Section: In Vitro Assaysmentioning

confidence: 99%

Toxic factors of Vibrio strains pathogenic to shrimp

Goarant¹,

Herlin²,

Brizard³

et al. 2000

Dis. Aquat. Org.

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ABSTRACT. Vibriosis is a major disease problem in shrimp aquaculture. 'Syndrome 93' is a seasonal juvenile vibriosis caused by Vibrio penaeicida which affects Litopenaeus stylirostris in grow-out ponds in New Caledonia. This study assessed the toxic activities of extracellular products (ECPs) from V. penaeicida, l/. alginolyticus and V, nigripulchritudo using in vivo injections in healthy juvenile L. stylirostris (= Penaeus styljrostns) and in vitro assays on shrimp primary cell cultures and the fish cell line epithelioma papulosum cyprini (EPC). Toxic effects of ECPs were demonstrated for all pathogenic Vibrio strains tested both in vivo and in vitro, but for shrimp only; no effect was observed on the fish cell line. ECP toxicity for New Caledonian V. penaeicida was found only after cultivation at low temperature (20°C) and not at higher temperature (30°C). This points to the fact that 'Syndrome 93' episodes are triggered by temperature drops. The assays used here demonstrate the usefulness of primary shrimp cell cultures to study virulence mechanisms of shrimp pathogenic bacteria.

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Section: In Vitro Assaysmentioning

confidence: 99%

Toxic factors of Vibrio strains pathogenic to shrimp

Goarant¹,

Herlin²,

Brizard³

et al. 2000

Dis. Aquat. Org.

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“…To date, several extracellular proteases belonging to V. harveyi have been identified: a 22-kDa cysteine protease, which is an important exotoxin (Liu et al, 1997;Liu and Lee, 1999), and three alkaline metal-chelator-sensitive proteases (Fukasawa et al, 1988a, b). Other species of Vibrio have been found to secrete serine (Park et al, 2009) and metalloproteases (Chang et al, 2005;Kwon et al, 2007) with fibrinolytic activities to proteins of the human clotting system.…”

Section: Discussionmentioning

confidence: 99%

Interaction of Pathogenic Vibrio Bacteria With the Blood Clot of the Pacific White Shrimp, Litopenaeus vannamei

Chaikeeratisak

Tassanakajon

Armstrong

2014

The Biological Bulletin

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Abstract.In addition to its roles in hemostasis and wound repair, the blood clot plays an underappreciated role in innate immunity, where the established clot serves as a barrier to microbial penetration into the internal milieu and where the early clot entraps and immobilizes microbes that have entered wounds to the integuments. In this report we document the behavior of the pathogenic gram-negative bacterium Vibrio harveyi that has been entrapped in the fabric of the extracellular blood clot of one of its target organisms, the Pacific white shrimp, Litopenaeus vannamei. The freshly entrapped bacteria are held tightly by the clot, losing even Brownian motility, but by 1 h post-entrapment, a fraction of the bacteria have established small domains of fibrinolysis that enlarge progressively, enabling bacteria to escape from the clot's embrace. Escape is dependent on the actions of both serine-and metallo-proteases released from the bacterial cells.

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“…Numerous virulence factors have been implicated in severe outbreaks of luminous vibriosis. The possession of toxic extracellular proteins such as proteases, phospholipases, haemolysins [10] and cysteine proteases [11] reportedly increase the virulence of this marine bacterium. In addition, virulence-related products such as siderophores [17], bacteriocins [12] resistance plasmids [6] and chitinases [25] have also been implicated in the virulence of Harveyi clade members.…”

Section: Introductionmentioning

confidence: 99%

Virulence Changes to Harveyi Clade Bacteria Infected with Bacteriophage from Vibrio owensii

Busico-Salcedo

Owens

2013

Indian J. Virol.

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Vibrio owensii is one of the most virulent vibrios known being able to kill crustacean larvae at 10 2 CFU ml -1 . This study describes virulence changes to naïve strains of Vibrio harveyi and Vibrio campbellii when infected with the bacteriophage VOB from a closely related species V. owensii 47666-1. The bacteriophage from V. owensii was induced into lytic phase by using mitomycin C at 100 ng ml -1 . One strain of V. harveyi and two strains of V. campbellii from 29 tested containing no prophage were susceptible to lysogenic conversion with VOB. Virulence changes induced in Harveyi clade bacteria included the upregulation of protein secretion, statistically significant increased haemolysin and chitinase production and increased mortality to nauplii of Penaeus monodon. No change in siderophore production was observed. Bacteriophage VOB is likely to be responsible for some of the virulence factors expressed by V. owensii. As this bacteriophage is able to infect strains of V. harveyi and V. campbellii this phage may contribute to increased virulence of other vibrios in aquaculture and in the natural environment.

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Cysteine protease is a major exotoxin of pathogenic luminousVibrio harveyiin the tiger prawn,Penaeusmonodon

Cited by 60 publications

References 22 publications

Toxic factors of Vibrio strains pathogenic to shrimp

Toxic factors of Vibrio strains pathogenic to shrimp

Interaction of Pathogenic Vibrio Bacteria With the Blood Clot of the Pacific White Shrimp, Litopenaeus vannamei

Virulence Changes to Harveyi Clade Bacteria Infected with Bacteriophage from Vibrio owensii

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