Cysteine protease gene expression and proteolytic activity during senescence of Alstroemeria petals

Wagstaff, Carol; Leverentz, Michael K.; Griffiths, Gareth; Thomas, Brian; Chanasut, Usawadee; Stead, Anthony D.; Rogers, Hilary J.

doi:10.1093/jexbot/53.367.233

Cited by 114 publications

(83 citation statements)

References 40 publications

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“…SEN1 and proteases are associated with senescence (Oh et al, 1996;Wagstaff et al, 2002). The reduction in the expression of the genes that encode these proteins in pi-1 and ap3-3 loss-of-function mutants correlates with the delayed senescence observed in these infertile mutants (VivianSmith et al, 2001; our unpublished observation).…”

Section: Stress-related Genesmentioning

confidence: 66%

Global Identification of Target Genes Regulated by APETALA3 and PISTILLATA Floral Homeotic Gene Action

2002

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Identifying the genes regulated by the floral homeotic genes APETALA3 ( AP3 ) and PISTILLATA ( PI ) is crucial for understanding the molecular mechanisms that lead to petal and stamen formation. We have used microarray analysis to conduct a broad survey of genes whose expression is affected by AP3 and PI activity. DNA microarrays consisting of 9216 Arabidopsis ESTs were screened with probes corresponding to mRNAs from different mutant and transgenic lines that misexpress AP3 and/or PI . The microarray results were further confirmed by RNA gel blot analyses. Our results suggest that AP3 and PI regulate a relatively small number of genes, implying that many genes used in petal and stamen development are not tissue specific and likely have roles in other processes as well. We recovered genes similar to previously identified petal-and stamen-expressed genes as well as genes that were not implicated previously in petal and stamen development. A very low percentage of the genes recovered encoded transcription factors. This finding suggests that AP3 and PI act relatively directly to regulate the genes required for the basic cellular processes responsible for petal and stamen morphogenesis.

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Section: Stress-related Genesmentioning

confidence: 66%

Global Identification of Target Genes Regulated by APETALA3 and PISTILLATA Floral Homeotic Gene Action

2002

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“…In15, a putative cysteine protease, showed similarity with PRT22 related to tepal senescence of Sandersonia (Eason et al 2002). In21, a putative cysteine protease with a granulin domain, was similar to several genes related to senescence, i.e., BoCP2 of broccoli (Coupe et al 2003), ALSCYP1 of Alstroemeria petals (Wagstaff et al 2002), SEN102 of daylily petals (Guerrero et al 1998), and DCCP1 of carnation petals (Jones et al 1995). The sequence of the putative cysteine protease In33 was similar to that of genes related to leaf senescence, i.e., SPG31 of sweet potato (Chen et al 2002), SAG12 of Arabidopsis (Noh and Amasino 1999a), and BnSAG12 of Brassica (Noh and Amasino 1999b).…”

Section: Appearance Of Senescence Parameters Compared With Gene Exprementioning

confidence: 96%

Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers

et al. 2007

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We isolated several senescence-associated genes (SAGs) from the petals of morning glory (Ipomoea nil) flowers, with the aim of furthering our understanding of programmed cell death. Samples were taken from the closed bud stage to advanced visible senescence. Actinomycin D, an inhibitor of transcription, if given prior to 4 h after opening, suppressed the onset of visible senescence, which occurred at about 9 h after flower opening. The isolated genes all showed upregulation. Two cell-wall related genes were upregulated early, one encoding an extensin and one a caffeoyl-CoA-3-O-methyltransferase, involved in lignin production. A pectinacetylesterase was upregulated after flower opening and might be involved in cell-wall degradation. Some identified genes showed high homology with published SAGs possibly involved in remobilisation processes: an alcohol dehydrogenase and three cysteine proteases. One transcript encoded a leucine-rich repeat receptor protein kinase, putatively involved in signal transduction. Another transcript encoded a 14-3-3 protein, also a protein kinase. Two genes have apparently not been associated previously with senescence: the first encoded a putative SEC14, which is required for Golgi vesicle transport, the second was a putative ataxin-2, which has been related to RNA metabolism. Induction of the latter has been shown to result in cell death in yeast, due to defects in actin filament formation. The possible roles of these genes in programmed cell death are discussed.

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“…Flower senescence is a highly regulated process that exhibits many of the structural, biochemical, and molecular changes that are hallmarks of programmed cell death. These include a loss of membrane permeability, increase in reactive oxygen species, and decreased levels of protective enzymes, followed by protein degradation, fatty acid breakdown, and degradation of nucleic acids (Wagstaff et al, 2002;Jones et al, 2005;Xu et al, 2006;Tripathi and Tuteja, 2007;Yamada et al, 2009;Shahri and Tahir, 2011;Rogers, 2013).…”

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confidence: 99%

An Ethylene-Induced Regulatory Module Delays Flower Senescence by Regulating Cytokinin Content

Jia

et al. 2016

Plant Physiol.

101

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ORCID IDs: 0000-0002-6288-7917 (Y.J.); 0000-0002-5972-7963 (C.-Z.J.); 0000-0002-3866-0894 (C.M.); 0000-0002-9285-2539 (J.G.).In many plant species, including rose (Rosa hybrida), flower senescence is promoted by the gaseous hormone ethylene and inhibited by the cytokinin (CTK) class of hormones. However, the molecular mechanisms underlying these antagonistic effects are not well understood. In this study, we characterized the association between a pathogenesis-related PR-10 family gene from rose (RhPR10.1) and the hormonal regulation of flower senescence. Quantitative reverse transcription PCR analysis showed that RhPR10.1 was expressed at high levels during senescence in different floral organs, including petal, sepal, receptacle, stamen, and pistil, and that expression was induced by ethylene treatment. Silencing of RhPR10.1 expression in rose plants by virusinduced gene silencing accelerated flower senescence, which was accompanied by a higher ion leakage rate in the petals, as well as increased expression of the senescence marker gene RhSAG12. CTK content and the expression of three CTK signaling pathway genes were reduced in RhPR10.1-silenced plants, and the accelerated rate of petal senescence that was apparent in the RhPR10.1-silenced plants was restored to normal levels by CTK treatment. Finally, RhHB6, a homeodomain-Leu zipper I transcription factor, was observed to bind to the RhPR10.1 promoter, and silencing of its expression also promoted flower senescence. Our results reveal an ethylene-induced RhHB6-RhPR10.1 regulatory module that functions as a brake of ethylenepromoted senescence through increasing the CTK content.

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Cysteine protease gene expression and proteolytic activity during senescence of Alstroemeria petals

Cited by 114 publications

References 40 publications

Global Identification of Target Genes Regulated by APETALA3 and PISTILLATA Floral Homeotic Gene Action

Global Identification of Target Genes Regulated by APETALA3 and PISTILLATA Floral Homeotic Gene Action

Gene expression in opening and senescing petals of morning glory (Ipomoea nil) flowers

An Ethylene-Induced Regulatory Module Delays Flower Senescence by Regulating Cytokinin Content

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