Cysteine Cathepsins in Human Dentin-Pulp Complex

Tersariol, Ivarne L.S.; Geraldeli, Saulo; Minciotti, Christiane L.; Nascimento, Fábio D.; Pääkkönen, Virve; Martins, Marília Trierveiler; Carrilho, Marcela Rocha de Oliveira; Pashley, David H.; Tay, Franklin R.; Salo, Tuula; Tjäderhane, Leo

doi:10.1016/j.joen.2009.12.034

Cited by 221 publications

(232 citation statements)

References 55 publications

(66 reference statements)

Supporting

Mentioning

188

Contrasting

Unclassified

Order By: Relevance

“…Previous studies also supported a positive correlation between the proteolytic activities of MMPs and cysteine cathepsins (Cox et al 2006). For these reasons, during the application of acidic monomers, not only MMPs but also cysteine cathepsins are activated and might be involved in the degradation of HL over time (Tersariol et al 2010;Tjäderhane et al 2013b).…”

Section: Role Of Mmps In Hl Degradationmentioning

confidence: 67%

See 1 more Smart Citation

Role of Dentin MMPs in Caries Progression and Bond Stability

et al. 2014

View full text Add to dashboard Cite

Dentin can be described as a biological composite with collagen matrix embedded with nanosized hydroxyapatite mineral crystallites. Matrix metalloproteinases (MMPs) and cysteine cathepsins are families of endopeptidases. Enzymes of both families are present in dentin and collectively capable of degrading virtually all extracellular matrix components. This review describes these enzymes and their presence in dentin, mainly focusing on their role in dentin caries pathogenesis and loss of collagen in the adhesive hybrid layer under composite restorations. MMPs and cysteine cathepsins present in saliva, mineralized dentin, and/or dentinal fluid may affect the dentin caries process at the early phases of demineralization. Changes in collagen and noncollagenous protein structure may participate in observed decreases in mechanical properties of caries-affected dentin and reduce the ability of caries-affected dentin to remineralize. These endogenous enzymes also remain entrapped within the hybrid layer during the resin infiltration process, and the acidic bonding agents themselves (irrespective of whether they are etch-and-rinse or self-etch) can activate these endogenous protease proforms. Since resin impregnation is frequently incomplete, denuded collagen matrices associated with free water (which serves as a collagen cleavage reagent for these endogenous hydrolase enzymes) can be enzymatically disrupted, finally contributing to the degradation of the hybrid layer. There are multiple in vitro and in vivo reports showing that the longevity of the adhesive interface is increased when nonspecific enzyme-inhibiting strategies are used. Different chemicals (i.e., chlorhexidine, galardin, and benzalkonium chloride) or collagen cross-linker agents have been successfully employed as therapeutic primers in the bonding procedure. In addition, the incorporation of enzyme inhibitors (i.e., quaternary ammonium methacrylates) into the resin blends has been recently promoted. This review will describe MMP functions in caries and hybrid layer degradation and explore the potential therapeutic role of MMP inhibitors for the development of improved intervention strategies for MMP-related oral diseases.

show abstract

Section: Role Of Mmps In Hl Degradationmentioning

confidence: 67%

“…In terms of protein expression, the variety of these proteases in the dentin-pulp complex has been claimed to be comparable with that described for MMPs (Palosaari et al 2003;Tersariol et al 2010;Tjäderhane et al 2013a). Similar to MMPs, cathepsins also participate in ECM degradation in physiological and pathological processes (Fig.…”

mentioning

confidence: 64%

Role of Dentin MMPs in Caries Progression and Bond Stability

et al. 2014

View full text Add to dashboard Cite

show abstract

“…37,58,73,74 It is, in addition, also capable of inhibiting cysteine cathepsins (CCs) 75 the other key class of proteolytic enzymes identified in dentin. 76 CHX was accordingly shown to partially conserve the integrity of the hybrid layer, 77 , favoring bond durability. CHX can be used as an antiproteolytic "primer" solution directly applied on the dentin surface, or after phosphoric acid etching and rinsing 74,78,79,80,81,82 or incorporated into the acid etching agent 83,84 or within the adhesive system composition.…”

Section: Enzymatic Inhibitorsmentioning

confidence: 99%

Bonding efficiency and durability: current possibilities

et al. 2017

View full text Add to dashboard Cite

Bonding plays a major role in dentistry nowadays. Dental adhesives are used in association with composites to solve many restorative issues. However, the wide variety of bonding agents currently available makes it difficult for clinicians to choose the best alternative in terms of material and technique, especially when different clinical situations are considered. Moreover, although bonding agents allow for a more conservative restorative approach, achieving a durable adhesive interface remains a matter of concern, and this mainly due to degradation of the bonding complex in the challenging oral environment. This review aims to present strategies that are being used or those still in development which may help to prevent degradation. It is fundamental that professionals are aware of these strategies to counteract degradation as much as possible. None of them are efficient to completely solve this problem, but they certainly represent reasonable alternatives to increase the lifetime of adhesive restorations.

show abstract

“…Detection of MMPs in mineralized dentin (Martin-De Las Heras et al, 2000;Sulkala et al, 2004Sulkala et al, , 2007Mazzoni et al, 2007Mazzoni et al, , 2011 and carious dentin (Tjäderhane et al, 1998;Chaussain-Miller et al, 2006) indicates that MMPs trapped within peripheral dentin may be activated once they are exposed by acid demineralization. The recent discovery of cysteine cathepsins in normal and carious dentin (Nascimento et al, 2011;Tersariol et al, 2010;Scaffa et al, 2012) increases the list of potential endogenous proteases in dentin matrices. Demineralization of the sound, unaffected collagen matrix exposes endogenous dentin proteases, although most of them remain bound to the collagen (Martin-De Las Heras et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of Phosphoric Acid on the Degradation of Human Dentin Matrix

et al. 2012

View full text Add to dashboard Cite

This study determined if dentin proteases are denatured by phosphoric acid (PA) used in etch-and-rinse dentin adhesives. Dentin beams were completely demineralized with EDTA for 30 days. We "acid-etched" experimental groups by exposing the demineralized dentin beams to 1, 10, or 37 mass% PA for 15 sec or 15 min. Control beams were not exposed to PA but were incubated in simulated body fluid for 3 days to assay their total endogenous telopeptidase activity, by their ability to solubilize C-terminal crosslinked telopeptides ICTP and CTX from insoluble dentin collagen. Control beams released 6.1 ± 0.8 ng ICTP and 0.6 ± 0.1 ng CTX/mg dry-wt/3 days. Positive control beams pre-incubated in p-aminophenylmercuric acetate, a compound known to activate proMMPs, released about the same amount of ICTP peptides, but released significantly less CTX. Beams immersed in 1, 10, or 37 mass% PA for 15 sec or 15 min released amounts of ICTP and CTX similar to that released by the controls (p > 0.05). Beams incubated in galardin, an MMP inhibitor, or E-64, a cathepsin inhibitor, blocked most of the release of ICTP and CTX, respectively. It is concluded that PA does not denature endogenous MMP and cathepsin activities of dentin matrices.

show abstract

Cysteine Cathepsins in Human Dentin-Pulp Complex

Cited by 221 publications

References 55 publications

Role of Dentin MMPs in Caries Progression and Bond Stability

Role of Dentin MMPs in Caries Progression and Bond Stability

Bonding efficiency and durability: current possibilities

Effect of Phosphoric Acid on the Degradation of Human Dentin Matrix

Contact Info

Product

Resources

About