Cystatin S in secretory granules fractions isolated from submandibular gland of infected rats by Trypanosoma cruzi

Silva, Gerluza A.B.; Alves, José Bento; Alves, Marlene

doi:10.1016/s0040-8166(95)80019-0

Cited by 6 publications

(2 citation statements)

References 28 publications

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“…Preparation of Salivary Granules and Their Extracts. Granules were isolated according to the procedure of Silva and co-workers with some modifications. The salivary gland (1.2 ± 0.2 g, N = 6, parotid tissue; 0.7 ± 0.4 g, N = 4, submandibular tissue) was washed with a 0.9% NaCl aqueous solution to eliminate blood, minced into small pieces by using a scalpel, and then homogenized in a glass potter with a Teflon pestle as a 5% (w/v) suspension in 340 mM sucrose, 0.5 mM EDTA, 10 mM HEPES, pH 7.4, at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Tyrosine Polysulfation of Human Salivary Histatin 1. A Post-Translational Modification Specific of the Submandibular Gland

et al. 2007

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Histatin 1 (His-1) derivatives showing serial mass increases of 80.0 +/- 0.1 Da were detected in human saliva by HPLC-ESI-MS. The same derivatives were also found in granules of submandibular glands and secretions of submandibular/sublingual glands, but not in granules and secretions of parotid glands. Only one phosphate group was present in His-1 and its derivatives, since treatment with alkaline phosphatase provided an 80.0 Da mass decrease. His-1 derivatives were almost completely transformed into His-1 by treatment with 1 M HCl at 100 degrees C, suggesting the presence of O-sulfotyrosine, which is more labile than phospho-Tyr to acidic hydrolysis. CE-MS analysis of pronase extensive digestion of derivatives confirmed the presence of sulfotyrosine. Derivatives were digested by trypsin, proteinase K, and protease V-8 and analyzed by different MS strategies. The results allowed locating sulfation on the last four tyrosines (Tyr 27, 30, 34, and 36). This study is the first report of the gland-specific sulfation of a salivary phosphopeptide in vivo.

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Section: Methodsmentioning

confidence: 99%

Tyrosine Polysulfation of Human Salivary Histatin 1. A Post-Translational Modification Specific of the Submandibular Gland

et al. 2007

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“…In fact, the observed enlargement of acinar cells may reflect the increased number and size of secretory granules, as well as altered expression of the secretory protein cystatin (Alves et al 1994, Silva et al 1995. We observed increased BrdU incorporation in the granular ducts in both groups, although, in the acute phase of Chagas disease, a higher incorporation can be observed in non-infected rats.…”

Section: Discussionmentioning

confidence: 57%

Delay in maturation of the submandibular gland in Chagas disease correlates with lower DNA synthesis

Alves

Napimoga

Araújo

et al. 2008

Mem. Inst. Oswaldo Cruz

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Mass spectrometry strategies applied to the characterization of proline‐rich peptides from secretory parotid granules of pig (Sus scrofa)

Fanali¹,

Inzitari²,

Cabras

et al. 2008

J of Separation Science

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Basic proline-rich proteins (bPRPs) are a class of proteins widely present in saliva of humans and other mammals. They are synthesized as preproproteins and enzymatically cleaved into small peptides before secretion from the salivary glands. Recently, we characterized two proline-rich peptides (SP-A and SP-B) in parotid secretory granules of pig (Sus Scrofa) that are derived from three isoforms of a PRP proprotein (Swiss-Prot data bank: Q95JC9-1, Q95JC9-2 and Q95JC9-3). Together the coding regions for SP-A and SP-B, which are repeated many times, account for 52-70% of the coding regions of the PRP proproteins. This study was undertaken to identify peptides encoded by unassigned regions of the PRP proproteins. RP-HPLC-ESI-IT-MS analysis of enriched granule preparations from pig parotid glands by two different analytical strategies identified ten new proline-rich peptides derived from the three proproteins. Together with the coding regions for SP-A and SP-B already identified it was possible to assign 68-75% of the proproteins coding regions. The peptide sequences indicated a number of unusual proteolytic cleavage sites suggesting the presence of unknown proprotein convertases.

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Cystatin S in secretory granules fractions isolated from submandibular gland of infected rats by Trypanosoma cruzi

Cited by 6 publications

References 28 publications

Tyrosine Polysulfation of Human Salivary Histatin 1. A Post-Translational Modification Specific of the Submandibular Gland

Tyrosine Polysulfation of Human Salivary Histatin 1. A Post-Translational Modification Specific of the Submandibular Gland

Delay in maturation of the submandibular gland in Chagas disease correlates with lower DNA synthesis

Mass spectrometry strategies applied to the characterization of proline‐rich peptides from secretory parotid granules of pig (Sus scrofa)

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