Cystathionine: A novel oncometabolite in human breast cancer

Sen, Suvajit; Kawahara, Brain; Mahata, Sushil K.; Tsai, Rebecca; Yoon, Alfred P.; Hwang, Lih Hwa; Hu-Moore, Kayla; Villanueva, Carissa; Vajihuddin, Abdulqadir; Parameshwar, Pooja S.; You, Michelle; Bhaskar, Divya Lakshmi; Gomez, O Cespedez; Faull, Kym F.; Farias‐Eisner, Robin; Chaudhuri, Gautam

doi:10.1016/j.abb.2016.06.010

Cited by 42 publications

(41 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Scientific reports describe the CBS and CTH roles in breast cancer models [16,37]. The CBS expression measured by different methods was described in breast cancer tissues by Sen et al [17], but they reported no CTH presence in the collected tissue samples. On the contrary, You et al [37] described the CTH expression in MCF-7 cells and provided the results supporting its significant role in the breast cancer development.…”

Section: Discussionmentioning

confidence: 99%

Effect of S-Allyl –L-Cysteine on MCF-7 Cell Line 3-Mercaptopyruvate Sulfurtransferase/Sulfane Sulfur System, Viability and Apoptosis

Bronowicka-Adamska

Bentke

Lasota

et al. 2020

IJMS

View full text Add to dashboard Cite

The S-Allyl-L-cysteine (SAC) component of aged garlic extract (AGE) is proven to have anticancer, antihepatotoxic, neuroprotective and neurotrophic properties. γ-Cystathionase (CTH), cystathionine β-synthase (CBS) and 3-mercaptopyruvate sulfurtransferase (MPST) are involved in H2S/sulfane sulfur endogenous formation from L-cysteine. The aim of the study was to determine the effect of SAC on MCF-7 cells survival and apoptosis, which is a widely known approach to reduce the number of cancer cells. An additional goal of this paper was to investigate the effect of SAC on the activity and expression of enzymes involved in H2S production. The experiments were carried out in the human breast adenocarcinoma cell line MCF-7. Changes in the cell viability were determined by MTT assay. Cell survival was determined by flow cytometry (FC). Changes in enzymes expression were analyzed using Western blot. After 24 h and 48 h incubation with 2245 µM SAC, induction of late apoptosis was observed. A decrease in cell viability was observed with increasing SAC concentration and incubation time. SAC had no significant cytotoxic effect on the MCF-7 cells upon all analyzed concentrations. CTH, MPST and CBS expression were confirmed in non-treated MCF-7 cells. Significant decrease in MPST activity at 2245 µM SAC after 24 h and 48 h incubation vs. 1000 µM SAC was associated with decrease in sulfane sulfur levels. The presented results show promising SAC effects regarding the deterioration of the MCF-7 cells’ condition in reducing their viability through the downregulation of MPST expression and sulfate sulfur level reduction.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of S-Allyl –L-Cysteine on MCF-7 Cell Line 3-Mercaptopyruvate Sulfurtransferase/Sulfane Sulfur System, Viability and Apoptosis

Bronowicka-Adamska

Bentke

Lasota

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…In general, the average cystathionine level in higher grade gliomas (II/III, III/IV, IV) was higher relative to low‐grade gliomas (II), with no genetic information provided . Abnormal accumulation of cystathionine in breast cancer was also reported, and cystathionine was suggested to be a novel oncometabolite possibly contributing to drug resistance in cancer cells.…”

Section: Introductionmentioning

confidence: 99%

In vivo ¹H MRS detection of cystathionine in human brain tumors

Branzoli

Deelchand

Sanson

et al. 2019

Magnetic Resonance in Med

View full text Add to dashboard Cite

Purpose To report the technical aspects of noninvasive detection of cystathionine in human brain glioma with edited MRS, and to investigate possible further acquisition improvements for robust quantification of this metabolite. Methods In vivo 1H MR spectra were acquired at 3 T in 15 participants with an isocitrate dehydrogenase‐mutated glioma using a MEGA‐PRESS (MEscher GArwood point resolved spectroscopy) sequence previously employed for 2‐hydroxyglutarate detection (TR = 2 s, TE = 68 ms). The editing pulse was applied at 1.9 ppm for the edit‐on condition and at 7.5 ppm for the edit‐off condition. To evaluate the editing efficiency, spectra were acquired in 1 participant by placing the editing pulse for the edit‐on condition at 1.9, 2.03, and 2.16 ppm. Cystathionine concentration was quantified using LCModel and a simulated basis set. To confirm chemical shifts and J‐coupling values of cystathionine, the 1H NMR cystathionine spectrum was measured using a high‐resolution 500 MHz spectrometer. Results In 12 gliomas, cystathionine was observed in the in vivo edited MR spectra at 2.72 and 3.85 ppm and quantified. The signal intensity of the cystathionine resonance at 2.72 ppm increased 1.7 and 2.13 times when the editing pulse was moved to 2.03 and 2.16 ppm, respectively. Cystathionine was not detectable in normal brain tissue. Conclusion Cystathionine can be detected in vivo by edited MRS using the same protocol as for 2‐hydroxyglutarate detection. This finding may enable a more accurate, noninvasive investigation of cellular metabolism in glioma.

show abstract

“…Recently, metabolomics, which analyzes all observable metabolites in biological samples, has emerged as a new promising tool for identification of therapeutic targets and diagnostic and prognostic markers in cancer research [ 5 ]. Recent studies have described essential metabolomic pathways in breast cancer and characterized oncometabolites that drove tumor growth and progression [ 6 – 8 ]. Furthermore, in order to obtain information on cellular function of metabolites, analyses of metabolites coupled with genetic or lipidomic profiling have been performed [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Development of suspension cell culture model to mimic circulating tumor cells

et al. 2017

View full text Add to dashboard Cite

Circulating tumor cells (CTCs) are essential for the establishment of distant metastasis. Numerous studies have characterized CTCs as metastatic precursors; however, the molecular nature of CTCs has not been completely revealed yet due to the low number of CTCs in the blood stream. As an alternative approach, we developed a long-term suspension cell culture model using human breast cancer cell lines to mimic CTCs. We found that more than 40 passaged suspension cells acquired the ability to enhance metastasis like cancer stem cells. To identify molecular changes acquired during the suspension cell culture, we analyzed metabolic and lipidomic profiles as well as transcriptome in MDA-MB-468 suspension cells. Glutamate and leucine levels increased in suspension cells, and cholesterol synthesis pathway was altered. The inhibition of glutamate metabolic pathway decreased the proliferation of suspension cells compared to that of adherent cells. In the lipidomic profile, PC species containing long chain and polyunsaturated fatty acids increased in suspension cells and these species could be authentic and specific biomarkers for highly metastatic cancers. As this CTC-mimicking suspension cell culture model may easily apply to various types of cancer, we suggest this model as a great tool to develop therapeutic targets and drugs to eradicate metastatic cancer cells.

show abstract

Cystathionine: A novel oncometabolite in human breast cancer

Cited by 42 publications

References 25 publications

Effect of S-Allyl –L-Cysteine on MCF-7 Cell Line 3-Mercaptopyruvate Sulfurtransferase/Sulfane Sulfur System, Viability and Apoptosis

Effect of S-Allyl –L-Cysteine on MCF-7 Cell Line 3-Mercaptopyruvate Sulfurtransferase/Sulfane Sulfur System, Viability and Apoptosis

In vivo ¹H MRS detection of cystathionine in human brain tumors

Development of suspension cell culture model to mimic circulating tumor cells

Contact Info

Product

Resources

About

Cystathionine: A novel oncometabolite in human breast cancer

Cited by 42 publications

References 25 publications

Effect of S-Allyl –L-Cysteine on MCF-7 Cell Line 3-Mercaptopyruvate Sulfurtransferase/Sulfane Sulfur System, Viability and Apoptosis

Effect of S-Allyl –L-Cysteine on MCF-7 Cell Line 3-Mercaptopyruvate Sulfurtransferase/Sulfane Sulfur System, Viability and Apoptosis

In vivo 1H MRS detection of cystathionine in human brain tumors

Development of suspension cell culture model to mimic circulating tumor cells

Contact Info

Product

Resources

About

In vivo ¹H MRS detection of cystathionine in human brain tumors