Cyromazine Effects the Reproduction of Drosophila by Decreasing the Number of Germ Cells in the Female Adult Ovary

Khalid, Muhammad Zaryab; Sun, Zhirong; Chen, Yaoyao; Zhang, Jing; Zhong, Guohua

doi:10.3390/insects13050414

Cited by 5 publications

(2 citation statements)

References 49 publications

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“…Measurements of Drosophila fecundity are used in a wide variety of studies, including investigations of aging, stem cell biology, nutrition, behavior, and toxicology [1][2][3][4][5][6][7]. These studies build on the wealth of knowledge about Drosophila oogenesis and the complex array of inputs that combine to optimize the rate of egg laying in response to the environment.…”

Section: Introductionmentioning

confidence: 99%

A high-throughput method for quantifyingDrosophilafecundity

Gomez,

Gonzalez,

Oke

et al. 2024

Preprint

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Measurements of Drosophila fecundity are used in a wide variety of studies, such as investigations of stem cell biology, nutrition, behavior, and toxicology. In addition, because fecundity assays are performed on live flies, they are suitable for longitudinal studies such as investigations of aging or prolonged chemical exposure. However, standard Drosophila fecundity assays have been difficult to perform in a high-throughput manner because experimental factors such as the physiological state of the flies and environmental cues must be carefully controlled to achieve consistent results. In addition, exposing flies to a large number of different experimental conditions (such as chemical additives in the diet) and manually counting the number of eggs laid to determine the impact on fecundity is time-consuming. We have overcome these challenges by combining a new multiwell fly culture strategy with a novel 3D-printed fly transfer device to rapidly and accurately transfer flies from one plate to another; the RoboCam, a low-cost, custom built robotic camera to capture images of the wells automatically; and an image segmentation pipeline to automatically identify and quantify eggs. We show that this method is compatible with robust and consistent egg laying throughout the assay period; and demonstrate that the automated pipeline for quantifying fecundity is very accurate (r2= 0.98 for the correlation between the automated egg counts and the ground truth) In addition, we show that this method can be used to efficiently detect the effects on fecundity induced by dietary exposure to chemicals. Taken together, this strategy substantially increases the efficiency and reproducibility of high throughput egg laying assays that require exposing flies to multiple different media conditions.

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Section: Introductionmentioning

confidence: 99%

A high-throughput method for quantifyingDrosophilafecundity

Gomez,

Gonzalez,

Oke

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Our previous study showed that the continuous selection of D. melanogaster from the larval to the adult stage affected the GSCs and CBs in the adult ovary. Furthermore, we observed that the expression of selected ecdysone signaling-related genes and ecdysone titer significantly decreased in the treated ovaries (Khalid et al, 2022). Therefore, to deeply investigate how this chemical affected the germ cells in the adult ovaries, we first counted the number of PGCs in the larval ovaries.…”

Section: Introductionmentioning

confidence: 99%

Cyromazine affects the ovarian germ cells of Drosophila via the ecdysone signaling pathway

et al. 2022

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Cyromazine, an insect growth regulator, has been extensively used against the insect pests of livestock and households. Previously, it was observed that the continuous selection of cyromazine from the larval to the adult stage decreased the number of germline stem cells (GSCs) and cystoblasts (CBs) in the adult ovary. In addition, in this study, we observed that the number of primordial germ cells (PGCs) was also decreased in the larval ovary after treatment with cyromazine. However, the mechanism by which it affects the germ cells is yet to be explored. Consequently, to deeply investigate the effects of cyromazine on the germ cells, we performed tissue-specific RNA sequencing. Bioinformatics analysis revealed that the ecdysone signaling pathway was significantly influenced under cyromazine stress. Based on that, we screened and selected 14 ecdysone signaling responsive genes and silenced their expression in the germ cells only. Results of that showed a considerable reduction in the number of germ cells. Furthermore, we mixed exogenous 20E with the cyromazine-containing diet to rescue the ecdysone signaling. Our results supported that the application of exogenous 20E significantly rescued the germ cells in the transgenic lines. Therefore, this implies that the cyromazine decreased the number of germ cells by affecting the ecdysone signaling pathway.

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Laboratory evaluation of insect growth regulators against the spotted wing drosophila, Drosophila suzukii

2023

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Drosophila suzukii (Diptera: Drosophilidae) is an invasive species that causes great damages to many stone and small fruits, resulting in reduced fruit production and high economic losses. The concern about this pest has led to the research of a wide range of possible control methods. However, the main control strategies are still based in insecticide applications. The efficacy of different insect growth regulators (IGRs), which have better ecotoxicological profile than conventional pesticides, was evaluated to test their feasibility for the control of this pest. When adult flies fed for three days on artificial diet surface-treated with lufenuron, cyromazine or pyriproxyfen, no adult offspring developed. However, adult offspring production progressively recovered when flies were transferred to non-treated diet. When adult flies fed continuously for 19 days on surface-treated artificial diet, lufenuron, cyromazine and pyriproxyfen completely suppressed the production of viable offspring while azadirachtin and tebufenozide reduced offspring production by more than a half compared to the untreated control. Nevertheless, these were transient non-permanent effects since viable offspring was produced shortly after females fed on treated diet were transferred to non-treated diet. The results observed in additional choice and egg-transfer assays indicate a vertical transmission of lufenuron and cyromazine from treated females to their offspring whereas the effect of pyriproxyfen seems to be related to the feeding of larvae on treated diet, although effects on reproductive processes cannot be discarded. In addition, choice assays showed no repellent effect on oviposition by lufenuron, cyromazine and pyriproxyfen.

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Cyromazine Effects the Reproduction of Drosophila by Decreasing the Number of Germ Cells in the Female Adult Ovary

Cited by 5 publications

References 49 publications

A high-throughput method for quantifyingDrosophilafecundity

A high-throughput method for quantifyingDrosophilafecundity

Cyromazine affects the ovarian germ cells of Drosophila via the ecdysone signaling pathway

Laboratory evaluation of insect growth regulators against the spotted wing drosophila, Drosophila suzukii

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