Cypovirus

Mertens, Peter P. C.; Attoui, Houssam; Sutton, Geoff

doi:10.1007/978-0-387-95919-1_259

Cited by 1 publication

(7 citation statements)

References 5 publications

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“…Reference strains of EHDV are shown in black and field isolates are shown in blue font. Isolate designations: IAH ‘dsRNA virus reference collection number composed of ‘country code, year, and the number of the isolate in that year from that country [48] , [49] . Full length Seg-2 sequences of Algerian and Moroccan 2006 strains were identical to each other and showed 97% nucleotide identity to the EHDV-6 [W]/BAR1983/01.…”

Section: Resultsmentioning

confidence: 99%

“…Full details of these isolates are presented in [15], and are also available on the dsRNA virus reference collection website [48].*Institute for Animal Health, Pirbright.**Serotypes listed are as reclassified by Anthony et al [15], with NIG1967/01 as serotype 1, not serotype 3. They have also characterised the unclassified strain ‘318’ as a western topotype of serotype 6 (EHDV-6[W]).…”

Section: Methodsmentioning

confidence: 99%

“…The different EHDV and other orbivirus isolates (listed in Table 1 and 2 ) were grown in BHK-21 cell monolayers, until 100% cytopathic effect (CPE) was observed. Each isolate is stored in the IAH ‘dsRNA virus reference collection’ (dsRNA-VRC) with a collection number composed of ‘country code, year, and the number of the isolate in that year from that country [48] , [49] . The samples used in these studies were taken from naturally infected animals in the field, by qualified veterinarians, as part of normal veterinary care and diagnostic testing procedures in the respective countries.…”

Section: Methodsmentioning

confidence: 99%

“… Full details of these isolates are presented in [15] , and are also available on the dsRNA virus reference collection website [48] . *Institute for Animal Health, Pirbright.…”

Section: Methodsmentioning

confidence: 99%

“… Full details of these isolates are available on the dsRNA website [49] . *Institute for Animal Health, Pirbright.…”

Section: Methodsmentioning

confidence: 99%

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RT-PCR Assays for Seven Serotypes of Epizootic Haemorrhagic Disease Virus & Their Use to Type Strains from the Mediterranean Region and North America

Maan

Nomikou

et al. 2010

PLoS ONE

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Epizootic haemorrhagic disease virus (EHDV) infects wild ruminants, causing a frequently fatal haemorrhagic disease. However, it can also cause bluetongue-like disease in cattle, involving significant levels of morbidity and mortality, highlighting a need for more rapid and reliable diagnostic assays. EHDV outer-capsid protein VP2 (encoded by genome-segment 2 [Seg-2]) is highly variable and represents the primary target for neutralising antibodies generated by the mammalian host. Consequently VP2 is also the primary determinant of virus “serotype”, as identified in virus neutralisation tests (VNT). Although previous reports have indicated eight to ten EHDV serotypes, recent serological comparisons and molecular analyses of Seg-2 indicate only seven EHDV “types”. Oligonucleotide primers were developed targeting Seg-2, for use in conventional RT-PCR assays to detect and identify these seven types. These assays, which are more rapid and sensitive, still show complete agreement with VNT and were used to identify recent EHDV isolates from the Mediterranean region and North America.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“… Full details of these isolates are presented in [15] , and are also available on the dsRNA virus reference collection website [48] . *Institute for Animal Health, Pirbright.…”

Section: Methodsmentioning

confidence: 99%

“… Full details of these isolates are available on the dsRNA website [49] . *Institute for Animal Health, Pirbright.…”

Section: Methodsmentioning

confidence: 99%

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