Cyphellophora and its relatives in Phialophora: biodiversity and possible role in human infection

Peng, Feng; Lu, Qiaoyun; Najafzadeh, Mohammad Javad; Ende, A. H. G. Gerrits van den; Sun, Jiufeng; Li, Ruoyu; Xi, Liyan; Vicente, Vânia A.; Wei, Lai; Lu, Chun; Hoog, G.S. de

doi:10.1007/s13225-012-0194-5

Cited by 62 publications

(71 citation statements)

References 35 publications

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“…Strains of C. guyanensis were united at a similarity level of 40%, and those of C. laciniata were at a level of 70%. Strain CBS 285.85, doubtfully assigned to C. pluriseptata by Feng et al (10), had a profile deviating from all strains analyzed, and the same holds true for an unidentified Cyphellophora strain, CBS 112.94. Products of the RCA reaction were visualized by electrophoresis on 1% agarose gels.…”

Section: Resultsmentioning

confidence: 74%

“…Distinction of species within the cutaneous group of agents in Cyphellophora and Phialophora ("europaea clade") (11) is based on multilocus sequence typing (MLST) data provided previously by Feng et al (10). Dendrograms derived from the AFLP banding patterns of strains analyzed were generated by the UPGMA algorithm (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Cultures are maintained in the reference collection of the Centraalbureau voor Schimmelcultures (CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands). Cultures were grown on oatmeal agar (OA) (Difco, Brunschwig, Amsterdam, The Netherlands) at 24°C for 1 week and identified down to the species level by partial sequencing of the internal transcribed spacer (ITS) region, the DNA-dependent RNA polymerase II largest subunit (RPB1), the partial ␤-tubulin gene (BT2), and the D1/D2 region of the nuclear large subunit ribosomal DNA (LSU rDNA) gene for a selection of the strains (10).…”

Section: Isolatesmentioning

confidence: 99%

“…Cyphellophora and its relatives in Phialophora are filamentous members of the group forming a well-supported clade within the order Chaetothyriales (10,11). Clinically the group is homogeneous because all species are recovered regularly as superficial colonizers or as causes of local invasive disease of human skin and nails (12).…”

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confidence: 99%

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Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

et al. 2013

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hThe species diversity and identification of black fungi belonging to Cyphellophora and Phialophora, which colonize and infect human skin and nails, were studied using amplified fragment length polymorphism (AFLP). A total of 76 Cyphellophora and Phialophora isolates were evaluated, and their delimitation was compared to earlier studies using multilocus sequencing. The results of the AFLP analysis and sequencing were in complete agreement with each other. Seven species-specific padlock probes for the most prevalent species were designed on the basis of the ribosomal DNA internal transcribed spacer region, and identification of the respective species could easily be achieved with the aid of rolling circle amplification.

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Section: Resultsmentioning

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Isolatesmentioning

confidence: 99%

mentioning

confidence: 99%

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Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

et al. 2013

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“…Molecular analyses have recently been applied to the taxonomy and phylogeny of black yeasts (Halici et al 2010, Nelsen et al 2011, Feng et al 2012, Lawrey et al 2012, Chomnunti et al 2013. This is because black yeasts are notoriously difficult to identify by morphology alone, due to their lack of significant differentiation, their pleomorphic growth, and their variable modes of conidiogenesis , Sterflinger 2006.…”

Section: Introductionmentioning

confidence: 99%

Knufia aspidiotus sp. nov., a new black yeast from scale insects

Bai

Sun

et al. 2013

Phytotaxa

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Three ascomycetes, isolated from the body cavity of the scale insect, Aspidiotus sp., collected in Song Mountain, Yanqing, Beijing, China, were identified to be a new species of Knufia. Knufia aspidiotus sp. nov. is introduced in this paper. The species is characterized by slow-growing, dematiaceous and blastic, endogenous conidia in undifferentiated hyphae, as well as darkly pigmented, enlarged multicellular bodies, and is similar to other Knufia species in morphology. Phylogenetic analyses, based on the small ribosomal subunit (SSU) gene and internal transcribed spacer (ITS) region, indicated that Knufia aspidiotus was related to, but clearly distant from other sequenced species in Knufia.

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Mycobial community assemblages in sink drains across a university campus

et al. 2022

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Multiple fungal species, including potential opportunistic pathogens have been previously identified in water systems. Here, we investigated over 250 restroom sink fungal communities across a university campus and evaluated their diversity and core taxa present. Remarkable similarity in mycobial community composition was observed across buildings with Ascomycota consistently dominating. We found a core mycobiome independent of the building sampled, that included Exophiala species, potential opportunistic pathogenic black yeasts. Other prevalent and dominant taxa included Saccharomyces and Fusarium, common built environment fungi. The frequent presence of Malassezia, a common skin commensal, showed the external influence of human activities as a source of fungi to sinks. The study represents a novel exploration of sink P‐traps mycobial communities from a public area and highlights their importance as reservoirs of possible pathogenic fungi, as well as emphasizing the relevance of further research in this understudied ecosystem within the built environment.

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Cyphellophora and its relatives in Phialophora: biodiversity and possible role in human infection

Cited by 62 publications

References 35 publications

Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification

Knufia aspidiotus sp. nov., a new black yeast from scale insects

Mycobial community assemblages in sink drains across a university campus

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