Cypermethrin- and fipronil-based insecticides cause biochemical changes in Physalaemus gracilis tadpoles

Rutkoski, Camila Fátima; Macagnan, Natani; Folador, Alexandre; Skovronski, Vrandrieli Jucieli; Amaral, Aline Monique Blank do; Leitemperger, Jossiele; Costa, Maiara; Hartmann, Paulo Afonso; Müller, Caroline; Loro, Vania Lúcia; Hartmann, Marília Teresinha

doi:10.1007/s11356-020-10798-w

Cited by 15 publications

(5 citation statements)

References 76 publications

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“…To attempt to elucidate whether and how the Sars-Cov-2 influence the aquatic animals, in this study, we investigate the same by adding the three produced synthetic peptides to mimic the resulting Covid-19 aquatic contamination in wastewater. The tadpole Physalaemus cuvieri , is a prevalent amphibian species found in many freshwater habitats throughout Brazil and South America ((Miranda et al, 2019; Herek et al, 2020; Araújo et al, 2020ab; Rutkoski et al, 2020). Its population stability and abundance in the areas that occur (Frost 2017), good adaptability in the laboratory, and early biological response justify the species’ choice (Herek et al, 2020; Araújo et al, 2020ab; Rutkoski et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The tadpole Physalaemus cuvieri , is a prevalent amphibian species found in many freshwater habitats throughout Brazil and South America ((Miranda et al, 2019; Herek et al, 2020; Araújo et al, 2020ab; Rutkoski et al, 2020). Its population stability and abundance in the areas that occur (Frost 2017), good adaptability in the laboratory, and early biological response justify the species’ choice (Herek et al, 2020; Araújo et al, 2020ab; Rutkoski et al, 2020). Previous studies using this species report the effects of water pollution caused by wastewater runoff (Wrubleswski et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Charlie-Silva

Araújo

Guimarães

et al. 2021

Preprint

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The Spike protein (S protein) is a critical component in the infection of the new coronavirus (SARS-CoV-2). The objective of this work was to evaluate whether peptides from S protein could cause negative impact in the aquatic animals. The aquatic toxicity of SARS-CoV-2 spike protein peptides derivatives has been evaluated in tadpoles (n = 50 tadpoles / 5 replicates of 10 animals) from species Physalaemus cuvieri (Leptodactylidae). After synthesis, purification, and characterization of peptides (PSDP2001, PSDP2002, PSDP2003) an aquatic contamination has been simulatedwith these peptides during 24 hours of exposure in two concentrations (100 and 500 ng/mL). The control group (“C”) was composed of tadpoles kept in polyethylene containers containing de-chlorinated water. Oxidative stress, antioxidant biomarkers and neurotoxicity activity were assessed. In both concentrations, PSPD2002 and PSPD2003 increased catalase and superoxide dismutase antioxidants enzymes activities, as well as oxidative stress (nitrite levels, hydrogen peroxide and reactive oxygen species). All three peptides also increased acetylcholinesterase activity in the highest concentration. These peptides showed molecular interactions in silico with acetylcholinesterase and antioxidant enzymes. Aquatic particle contamination of SARS-CoV-2 has neurotoxics effects in P. cuvieri tadpoles. These findings indicate that the COVID-19 can constitute environmental impact or biological damage potential.HIGHLIGHTSSARS-CoV-2 spike protein peptides (PSDP) were synthesized, purified, and characterized by solid phase peptide synthesis.PSDP peptides promoted REDOX imbalance and acute neurotoxicity in tadpoles (Physalaemus cuvieri)In silico studies have shown interactionsbetween peptides and acetylcholinesterase and antioxidant enzymesAquatic particle contamination of SARS-CoV-2 can constitute additional environmental damageGRAPHICAL ABSTRACT

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

Charlie-Silva

Araújo

Guimarães

et al. 2021

Preprint

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“…After assessing for inclusion (Figure S1), we extracted 2,012 effect sizes from 86 studies (Acquaroni et al, 2021; Anguiano et al, 2001; Attademo et al, 2016; Awadalla et al, 2019; Barreto et al, 2020; Bhuyan et al, 2020; Burraco and Gomez-Mestre, 2016; Carvalho et al, 2020; Chai et al, 2017; Cheng et al, 2017; Cheron et al, 2022; Costa et al, 2008; Czarniewska et al, 2003; da Silva et al, 2021; David et al, 2012; De Lima Coltro et al, 2017; Dornelles and Oliveira, 2016; Ejilibe et al, 2018; Ezemonye and Tongo, 2010; Falfushynska et al, 2015; Falfushynska et al, 2017; Ferrari et al, 2008; Ferrari et al, 2009; Ferrari et al, 2011; Freitas et al, 2017; Gillardin et al, 2009; Huang et al, 2007; Isnas et al, 2012; Jiang et al, 2019; Jones et al, 2010; Kanter and Celik, 2012; Kostaropoulos et al, 2005; Lajmanovich et al, 2018b; Lajmanovich et al, 2018a; Lajmanovich et al, 2022; Li et al, 2017; Li et al, 2018a; Li et al, 2018b; Liendro et al, 2015; Liu et al, 2006; Liu et al, 2021; Loumbourdis, 2006; Lu et al, 2021; Mardirosian et al, 2015; Marques et al, 2013; Martins et al, 2017; Melvin, 2016; Mussi and Calcaterra, 2010; Naab et al, 2001; Nascimento et al, 2021; Nasia et al, 2018; Özkol et al, 2012; Pal et al, 2018; Papadimitriou and Loumbourdis, 2002; Peltzer et al, 2019; Radovanović et al, 2017; Radovanović et al, 2021; Rosenbaum et al, 2012; Rutkoski et al, 2020; Rutkoski et al, 2021; Saad et al, 2022; Salvaterra et al, 2013; Saria et al, 2014; Shi et al, 2018; Sotomayor et al, 2015; Svartz et al, 2020; Tang et al, 2018; Trachantong et al, 2017;…”

Section: Methodsmentioning

confidence: 99%

The impact of chemical pollution across major life transitions: a meta-analysis on oxidative stress in amphibians

Martin,

Capilla-Lasheras,

Monaghan

et al. 2023

Preprint

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Among human actions threatening biodiversity, the release of anthropogenic chemical pollutants -which have become ubiquitous in the environment- is a major concern. Chemical pollution can induce oxidative stress and damage by causing the overproduction of reactive oxygen species (ROS), and affecting the antioxidant system. In species undergoing metamorphosis (~80% of all extant animal species), antioxidant responses to chemical pollution may differ between pre- and post-metamorphic stages. Here, we meta-analysed (N = 86 studies, k = 2,012 estimates) the impact of chemical pollution on the redox balance across the three major amphibian life stages (embryo, larva, adult). Before metamorphosis, embryos and larvae activate their antioxidant pathways and do not show increased oxidative damage. In contrast, post-metamorphic individuals show unnoticeable antioxidant responses, and a marked oxidative damage in lipids. Also, type of pollutant (i.e., organic vs inorganic) promotes contrasting effects across amphibian life stages. Our findings show a divergent evolution of the redox balance in response to pollutants across life transitions of metamorphosing amphibians, likely linked to the characteristics of each life stage. Further comparative mechanistic approaches to wildlife responses to global changes will improve our understanding of these eco-evo-devo processes.

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“…We used tadpoles belonging to species P. cuvieri (Anura, Leptodactylidae) as model system to assess the aquatic toxicity of CNFs. Its wide geographical distribution in South America (Miranda et al, 2019), stability and population abundance in the occupied areas (Frost, 2017), good adaptability to laboratory environment, early biological response to changes in its environment, and use in recent (eco) toxicological studies justify their choice as model in our study (Herek et al, 2020; Araújo et al, 2020ab; Rutkoski et al, 2020). All tadpoles used in the experiment came from an ovigerous mass with approximately 1,500 eggs, based on Pupin et al (2010).…”

Section: Methodsmentioning

confidence: 99%

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

Guimarães

Estrela

Rodrigues

et al. 2021

Preprint

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Although carbon nanotubes (CNTs) toxicity in different experimental systems (in vivo and in vitro) is known, little is known about the toxic effects of carbon nanofibers (CNFs) on aquatic vertebrates. We herein investigated the potential impact of CNFs (1 and 10 mg/L) by using Physalaemus cuvieri tadpoles as experimental model. CNFs were able to induce nutritional deficit in animals after 48-h exposure to them, and this finding was inferred by reductions observed in body concentrations of total soluble carbohydrates, total proteins, and triglycerides. The increased production of hydrogen peroxide, reactive oxygen species and thiobarbituric acid reactive substances in tadpoles exposed to CNFs has suggested REDOX homeostasis change into oxidative stress. This process was correlated to the largest number of apoptotic and necrotic cells in the blood of these animals. On the other hand, the increased superoxide dismutase and catalase activity has suggested that the antioxidant system of animals exposed to CNFs was not enough to maintain REDOX balance. In addition, CNFs induced increase in acetylcholinesterase and butyrylcholinesterase activity, as well as changes in the number of neuromats evaluated on body surface (which is indicative of the neurotoxic effect of nanomaterials on the assessed model system). To the best of our knowledge, this is the first report on the impact of CNFs on amphibians; therefore, it broadened our understanding about ecotoxicological risks associated with their dispersion in freshwater ecosystems and possible contribution to the decline in the populations of anurofauna species.

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Cypermethrin- and fipronil-based insecticides cause biochemical changes in Physalaemus gracilis tadpoles

Cited by 15 publications

References 76 publications

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

An insight into neurotoxic and toxicity of spike fragments SARS-CoV-2 by exposure environment: A threat to aquatic health?

The impact of chemical pollution across major life transitions: a meta-analysis on oxidative stress in amphibians

Can Carbon Nanofibers Affect Anurofauna? Study Involving NeotropicalPhysalaemus cuvieri(Fitzinger, 1826) Tadpoles

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