CYP81A68 confers metabolic resistance to ALS and ACCase-inhibiting herbicides and its epigenetic regulation in Echinochloa crus-galli

Pan, Lang; Guo, Qiushuang; Wang, Junzhi; Shi, Li; Yang, Xiao; Zhou, Yaoyu; Yu, Qin; Bai, Lianyang

doi:10.1016/j.jhazmat.2022.128225

Cited by 59 publications

(61 citation statements)

References 81 publications

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“…For example, Arabidopsis or Oryza sativa L. expressing E. phyllopogon P450 genes ( CYP81A12, CYP81A14, CYP81A15, CYP81A18, CYP81A21, CYP81A24, CYP81A63 ) display metabolic ability for herbicides of ALS-inhibiting herbicides (pyrazosulfuron-ethyl, bensulfuron-methyl, penoxsulam, pyriftalid, pyrimisulfan, propyrisulfuron, chlorsulfuron, azimsulfuron or propoxycarbazone-sodium), ACCase-inhibiting herbicides (diclofop-methyl, tralkoxydim, pinoxaden), Deoxy-D-xyulose phosphate synthase-inhibiting herbicide (clomazone), PSII-inhibiting herbicide (bentazone), HPPD-inhibiting herbicide (mesotrione), PPO-inhibiting herbicide (pyraclonil) or (and) PDS-inhibiting herbicide (norflurazon) [ 30 , 31 , 32 , 33 ]. O. sativa transferring the E. crus-galli CYP81A68 gene has shown resistance to an ALS-inhibiting herbicide (penoxsulam) and ACCase-inhibiting herbicides (cyhalofop-butyl and metamifop) [ 34 ]. Another O. sativa transferring the L. rigidum CYP81A10v7 gene became highly resistant to ACCase- and ALS-inhibiting herbicides (diclofop-methyl, tralkoxydim, chlorsulfuron) and moderately resistant to HPPD-inhibiting herbicide trifluralin [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

The Metabolism of a Novel Cytochrome P450 (CYP77B34) in Tribenuron-Methyl-Resistant Descurainia sophia L. to Herbicides with Different Mode of Actions

Shen

Yang

Hao

et al. 2022

IJMS

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Descurainia sophia L. (flixweeds) is a noxious broad-leaf weed infesting winter wheat fields in China that has evolved high resistance to tribenuron-methyl. In this work, a brand new gene CYP77B34 was cloned from tribenuron-methyl-resistant (TR) D. sophia and transferred into Arabidopsis thaliana, and the sensitivities of Arabidopsis with or without the CYP77B34 transgene to herbicides with a different mode of actions (MoAs) were tested. Compared to Arabidopsis expressing pCAMBIA1302-GFP (empty plasmid), Arabidopsis transferring pCAMBIA1302-CYP77B34 (recombinant plasmid) became resistant to acetolactate synthase (ALS)-inhibiting herbicide tribenuron-methyl, protoporphyrinogen oxidase (PPO)-inhibiting herbicides carfentrazone-ethyl and oxyfluorfen. Cytochrome P450 inhibitor malathion could reverse the resistance to tribenuron-methyl, carfentrazone-ethyl and oxyfluorfen in transgenic Arabidopsis plants. In addition, the metabolic rates of tribenuron-methyl in Arabidopsis expressing CYP77B34 were significantly higher than those in Arabidopsis expressing pCAMBIA1302-GFP. Other than that, the transgenic plants showed some tolerance to very-long-chain fatty acid synthesis (VLCFAs)-inhibiting herbicide pretilachlor and photosystem (PS) II-inhibiting herbicide bromoxynil. Subcellular localization revealed that the CYP77B34 protein was located in the endoplasmic reticulum (ER). These results clearly indicated that CYP77B34 mediated D. sophia resistance to tribenuron-methyl and may have been involved in D. sophia cross-resistance to carfentrazone-ethyl, oxyfluorfen, pretilachlor and bromoxynil.

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Section: Discussionmentioning

confidence: 99%

The Metabolism of a Novel Cytochrome P450 (CYP77B34) in Tribenuron-Methyl-Resistant Descurainia sophia L. to Herbicides with Different Mode of Actions

Shen

Yang

Hao

et al. 2022

IJMS

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“…Higher-expression-level CYP709C56 degrades mesosulfuron-methyl in A. aequalis [ 17 ], and CYP77B34 from Descurainia sophia can confer Arabidopsis resistance to tribenuron-methyl, bromoxynil and pretilachlor [ 30 ]. Weeds are good materials for providing genetic resources for herbicide-resistant crops, and scientists have identified several CYP81A subfamily genes ( CYP81A12, CYP81A14, CYP81A15, CYP81A18, CYP81A21, CYP81A24, and CYP81A63 ) that metabolize herbicides only in E. phyllopogon [ 3 , 15 , 16 , 33 ], and CYP81A68 endows generalist metabolic resistance to penoxsulam and cyhalofop-butyl in Echinochloa crus-galli [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Cytochrome P450 BsCYP99A44 and BsCYP704A177 Confer Metabolic Resistance to ALS Herbicides in Beckmannia syzigachne

Bai

Yin

Lyu

et al. 2022

IJMS

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Beckmannia syzigachne is a noxious grassy weed that infests wheat fields in China. Previously, we identified that mesosulfuron-methyl resistance in a B. syzigachne population (R, SD04) was conferred by non-target resistance, such as cytochrome P450 mixed-function oxidases (P450s)-based metabolism. RNA sequencing and real-time PCR (qRT-PCR) were used to discover potential P450s-resistant-related genes. Five cytochrome P450s (CYP704A177, CYP96B84, CYP71D7, CYP93A1, and CYP99A44) were found to be highly expressed in R plants. In this study, CYP99A44 and CYP704A177 were cloned from B. syzigachne and transferred into Arabidopsis thaliana to test the sensitivity of Arabidopsis with and without P450s genes to mesosulfuron-methyl and other acetolactate synthase (ALS)-inhibiting herbicides. Transgenic Arabidopsis overexpressing CYP99A44 became resistant to the sulfonylurea herbicide mesosulfuron-methyl, but showed no resistance to pyroxsulam, imazethapyr, flucarbazone, and bispyribac-sodium. Notably, those overexpressing CYP704A177 showed resistance to pyroxsulam and bispyribac-sodium, but not to mesosulfuron-methyl, imazethapyr, and flucarbazone. These results indicated that B. syzigachne and transgenic Arabidopsis displayed different cross-resistance patterns to ALS-inhibiting herbicides. Subcellular localization revealed that CYP99A44 and CYP704A177 protein were located in the endoplasmic reticulum. Furthermore, these results clearly indicated that CYP99A44-mediated mesosulfuron-methyl resistance in B. syzigachne and CYP704A177 may be involved in B. syzigachne cross-resistance to pyroxsulam and bispyribac-sodium.

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“…Penoxsulam is a post-emergence triazolopyrimidine sulphonamide herbicide registered for weed control in rice crops [ 39 , 40 ] and bensulfuron-methyl is a representative sulfonylurea herbicide that controls broadleaf weeds [ 41 ]. Both of them could inhibit acetolactate synthase (ALS) activity in susceptible plant species, and application alone easily produced herbicide resistance in agricultural practices [ 42 , 43 ]. The integration application of different action modes of bioherbicide agent B. eleusines could potentially delay the development of herbicide resistance and greatly extend the shelf life of the herbicide.…”

Section: Discussionmentioning

confidence: 99%

Improved Bioherbicidal Efficacy of Bipolaris eleusines through Herbicide Addition on Weed Control in Paddy Rice

Zhang

Duan²,

Yang

et al. 2022

Plants

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Bipolaris eleusines was mixed with herbicides to improve the control of barnyardgrass (Echinochloa crus-galli), a noxious weed in rice fields. The compatibility of B. eleusines with herbicides was evaluated for toxic effects on spore germination and mycelium growth in vitro tests, and varied effects were observed with different chemical products. Briefly, 25 g/L penoxsulam OD plus 10% bensulfuron-methyl WP were much more compatible with B. eleusines, and there was no inhibition of spore germination but the promotion of mycelium growth of B. eleusines at all treatment rates. Under greenhouse conditions, the coefficient of the specificity of B. eleusines conidial agent was determined as 3.91, closer to the herbicidal control of 2.89, showing it is highly specific between rice and barnyardgrass. Field experiments in 2011 and 2012 showed that B. eleusines conidial agent displayed good activity on barnyardgrass, monochoria [Monochoria vaginalis (Burm.f.) Presl. Ex Kunth.], and small-flower umbrella sedge (Cyperus difformis L.) and had no negative impact on the rice plant. It also reduced the loss of rice yield when compared with the non-treated control and could make this pathogen a conidial agent for commercial bioherbicidal development in the future.

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CYP81A68 confers metabolic resistance to ALS and ACCase-inhibiting herbicides and its epigenetic regulation in Echinochloa crus-galli

Cited by 59 publications

References 81 publications

The Metabolism of a Novel Cytochrome P450 (CYP77B34) in Tribenuron-Methyl-Resistant Descurainia sophia L. to Herbicides with Different Mode of Actions

The Metabolism of a Novel Cytochrome P450 (CYP77B34) in Tribenuron-Methyl-Resistant Descurainia sophia L. to Herbicides with Different Mode of Actions

Cytochrome P450 BsCYP99A44 and BsCYP704A177 Confer Metabolic Resistance to ALS Herbicides in Beckmannia syzigachne

Improved Bioherbicidal Efficacy of Bipolaris eleusines through Herbicide Addition on Weed Control in Paddy Rice

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