CYP79D73 Participates in Biosynthesis of Floral Scent Compound 2-Phenylethanol in <i>Plumeria rubra</i>

Dhandapani, Savitha; Jin, Jingjing; Sridhar, Vishweshwaran; Chua, Nam‐Hai; Jang, In‐Cheol

doi:10.1104/pp.19.00098

Cited by 34 publications

(54 citation statements)

References 49 publications

(89 reference statements)

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“…This result further confirms that aldoximes or their derivatives, not glucosinolates, are key molecules affecting transcriptional activation of KFBs. Recent studies have identified aldoximeproducing enzymes in various species including monocots and gymnosperms (Irmisch et al, 2013;Irmisch et al, 2015;Luck et al, 2017;Bhalla et al, 2018;Dhandapani et al, 2019). Considering that aldoxime metabolism is widely spread in the plant kingdom, this type of link may exist in species beyond Brassicales.…”

Section: Altered Iaox Metabolism Affects Phenylpropanoid Production Tmentioning

confidence: 99%

“…The aldoximes are made from various amino acids by the cytochrome P450 monooxygenase 79 family members (CYP79) (Irmisch et al, 2013;Irmisch et al, 2015;Luck et al, 2017;Bhalla et al, 2018;Sørensen et al, 2018;Dhandapani et al, 2019). Arabidopsis thaliana has five functional CYP79s.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, IAOx is a precursor of indole-glucosinolates, IAA, and camalexin (Zhao et al, 2002;Sugawara et al, 2009;Klein et al, 2013) ( Figure 1A), therefore overexpression of AtCYP79B2 increases the production of IAA and indole glucosionolates (Zhao et al, 2002). Since aldoximes are precursors of various metabolites in addition to glucosinolates (Irmisch et al, 2013;Irmisch et al, 2015;Luck et al, 2017;Bhalla et al, 2018;Sørensen et al, 2018;Dhandapani et al, 2019) and the phenylpropanoid pathway is conserved in most plants, it is possible that a similar interaction occurs in other plant species. This study aims to elucidate a connection between IAOx metabolism and the phenylpropanoid pathway in Camelina sativa ( Figure 1A).…”

Section: Introductionmentioning

confidence: 99%

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Aldoxime Metabolism Is Linked to Phenylpropanoid Production in Camelina sativa

et al. 2020

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Plants produce diverse secondary metabolites. Although each metabolite is made through its respective biosynthetic pathway, plants coordinate multiple biosynthetic pathways simultaneously. One example is an interaction between glucosinolate and phenylpropanoid pathways in Arabidopsis thaliana. Glucosinolates are defense compounds made primarily from methionine and tryptophan, while phenylpropanoids are made from phenylalanine. Recent studies have shown that the accumulation of glucosinolate intermediate such as indole-3-acetaldoxime (IAOx) or its derivatives represses phenylpropanoid production via the degradation of phenylalanine ammonia lyase (PAL) functioning at the entry point of the phenylpropanoid pathway. Given that IAOx is a precursor of other bioactive compounds other than glucosinolates and that the phenylpropanoid pathway is present in most plants, we hypothesized that this interaction is relevant to other species. Camelina sativa is an oil crop and produces camalexin from IAOx. We enhanced IAOx production in Camelina by overexpressing Arabidopsis CYP79B2 which encodes an IAOx-producing enzyme. The overexpression of AtCYP79B2 results in increased auxin content and its associated morphological phenotypes in Camelina but indole glucosinolates were not detected in Camelina wild type as well as the overexpression lines. However, phenylpropanoid contents were reduced in AtCYP79B2 overexpression lines suggesting a link between aldoxime metabolism and phenylpropanoid production. Interestingly, the expression of PALs was not affected in the overexpression lines although PAL activity was reduced. To test if PAL degradation is involved in the crosstalk, we identified F-box genes functioning in PAL degradation through a phylogenetic study. A total of 459 transcript models encoding kelch-motifs were identified from the Camelina sativa database. Among them, the expression of CsKFBs involved in PAL degradation is up-regulated in the transgenic lines. Our results suggest a link between aldoxime metabolism and phenylpropanoid production in Camelina and that the molecular mechanism behind the crosstalk is conserved in Arabidopsis and Camelina.

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Section: Altered Iaox Metabolism Affects Phenylpropanoid Production Tmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aldoxime Metabolism Is Linked to Phenylpropanoid Production in Camelina sativa

et al. 2020

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“…2) (91). 2-Phenylethanol, a typical yeast odorant (see, for example, reference 92), is also produced by green plants (see, for example, reference 93). In addition to an overlap of compounds produced by both plants and yeasts, fumigation of elderberry flowers with broad-spectrum antibiotics revealed that floral phyllospheric microbiota are unique producers of key floral terpenes (20).…”

Section: Discussionmentioning

confidence: 99%

Yeast Volatomes Differentially Affect Larval Feeding in an Insect Herbivore

Ljunggren

Borrero‐Echeverry

Chakraborty

et al. 2019

Appl Environ Microbiol

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Yeasts interface insect herbivores with their food plants. Communication depends on volatile metabolites, and decoding this chemical dialogue is key to understanding the ecology of insect-yeast interactions. This study explores the volatomes of eight yeast species which have been isolated from foliage, from flowers or fruit, and from plant-feeding insects. These yeasts each release a rich bouquet of volatile metabolites, including a suite of known insect attractants from plant and floral scent. This overlap underlines the phylogenetic dimension of insect-yeast associations, which according to the fossil record long predate the appearance of flowering plants. Volatome composition is characteristic for each species, aligns with yeast taxonomy, and is further reflected by a differential behavioral response of cotton leafworm larvae, which naturally feed on foliage of a wide spectrum of broad-leaved plants. Larval discrimination may establish and maintain associations with yeasts and is also a substrate for designing sustainable insect management techniques.

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“…Because aldoximes are precursors of many other biomolecules besides glucosinolates, including cyanogenic glucosides and nitriles, and can be found in diverse plant species such as maize, poplar, yew and coca (Irmisch et al, 2013(Irmisch et al, , 2015Luck et al, 2017;Bhalla et al, 2018;Sørensen et al, 2018;Dhandapani et al, 2019), aldoxime-mediated suppression of phenylpropanoid metabolism may be relevant to other species and metabolic pathways beyond Arabidopsis. Previous work showed that glucosinolate/phenylpropanoid crosstalk requires MED5 (Kim et al, 2015), but its underlying mechanism was not identified.…”

Section: Discussionmentioning

confidence: 99%

Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome‐dependent degradation of PAL

et al. 2019

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Summary Plants produce several hundreds of thousands of secondary metabolites that are important for adaptation to various environmental conditions. Although different groups of secondary metabolites are synthesized through unique biosynthetic pathways, plants must orchestrate their production simultaneously. Phenylpropanoids and glucosinolates are two classes of secondary metabolites that are synthesized through apparently independent biosynthetic pathways. Genetic evidence has revealed that the accumulation of glucosinolate intermediates limits phenylpropanoid production in a Mediator Subunit 5 (MED5)‐dependent manner. To elucidate the molecular mechanism underlying this process, we analyzed the transcriptomes of a suite of Arabidopsis thaliana glucosinolate‐deficient mutants using RNAseq and identified misregulated genes that are rescued by the disruption of MED5. The expression of a group of Kelch Domain F‐Box genes (KFBs) that function in PAL degradation is affected in glucosinolate biosynthesis mutants and the disruption of these KFBs restores phenylpropanoid deficiency in the mutants. Our study suggests that glucosinolate/phenylpropanoid metabolic crosstalk involves the transcriptional regulation of KFB genes that initiate the degradation of the enzyme phenylalanine ammonia‐lyase, which catalyzes the first step of the phenylpropanoid biosynthesis pathway. Nevertheless, KFB mutant plants remain partially sensitive to glucosinolate pathway mutations, suggesting that other mechanisms that link the two pathways also exist.

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CYP79D73 Participates in Biosynthesis of Floral Scent Compound 2-Phenylethanol in Plumeria rubra

Cited by 34 publications

References 49 publications

Aldoxime Metabolism Is Linked to Phenylpropanoid Production in Camelina sativa

Aldoxime Metabolism Is Linked to Phenylpropanoid Production in Camelina sativa

Yeast Volatomes Differentially Affect Larval Feeding in an Insect Herbivore

Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome‐dependent degradation of PAL

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