CYP35 family in Caenorhabditis elegans biological processes: fatty acid synthesis, xenobiotic metabolism, and stress responses

Lim, Sharoen Yu Ming; Alshagga, Mustafa Ahmed; Kong, Cin; Alshawsh, Mohammed Abdullah; Alshehade, Salah Abdalrazak; Pan, Yan

doi:10.1007/s00204-022-03382-3

Cited by 8 publications

(5 citation statements)

References 89 publications

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“…After confirming the low cytotoxicity of the fractions, three concentrations were chosen to investigate the intracellular fat accumulation directly in the cells using a fluorometer and compared to fatty acids mixture (i.e., oleic and palmitic acids), it was reported that an increased uptake of oleic and elaidic acids resulted in altered expression of fat metabolism genes [ 49 ]. It was surprising that only the more polar fractions (ethanolic, hydroethanolic and water) had effects, particularly after 24 h of treatment.…”

Section: Discussionmentioning

confidence: 99%

Orthosiphon aristatus (Blume) Miq Alleviates Non-Alcoholic Fatty Liver Disease via Antioxidant Activities in C57BL/6 Obese Mice and Palmitic–Oleic Acid-Induced Steatosis in HepG2 Cells

et al. 2023

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Non-alcoholic fatty liver disease (NAFLD) is the most prevalent form of liver disease. Orthosiphon aristatus (Blume) Miq, a traditional plant in South Asia, has previously been shown to attenuate obesity and hyperglycaemic conditions. Eight weeks of feeding C57BL/6 mice with the standardized O. aristatus extract (400 mg/kg) inhibited the progression of NAFLD. Liver enzymes including alanine aminotransferase and aspartate transaminase were significantly reduced in treated mice by 74.2% ± 7.69 and 52.8% ± 7.83, respectively. Furthermore, the treated mice showed a reduction in serum levels of glucose (50% ± 5.71), insulin (70.2% ± 12.09), total cholesterol (27.5% ± 15.93), triglycerides (63.2% ± 16.5), low-density lipoprotein (62.5% ± 4.93) and atherogenic risk index relative to the negative control. Histologically, O. aristatus reversed hepatic fat accumulation and reduced NAFLD severity. Notably, our results showed the antioxidant activity of O. aristatus via increased superoxide dismutase activity and a reduction of hepatic malondialdehyde levels. In addition, the levels of serum pro-inflammatory mediators (IL-6 and TNFα) decreased, indicating anti-inflammatory activity. The aqueous, hydroethanolic and ethanolic fractions of O. aristatus extract significantly reduced intracellular fat accumulation in HepG2 cells that were treated with palmitic–oleic acid. Together, these findings suggest that antioxidant activities are the primary mechanism of action of O. aristatus underlying the anti-NAFLD effects.

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Section: Discussionmentioning

confidence: 99%

Orthosiphon aristatus (Blume) Miq Alleviates Non-Alcoholic Fatty Liver Disease via Antioxidant Activities in C57BL/6 Obese Mice and Palmitic–Oleic Acid-Induced Steatosis in HepG2 Cells

et al. 2023

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“…Consistent with our results, the expressions of cyp35 genes in nematodes could also be up-regulated by pesticides, heavy metals, and polycyclic aromatic hydrocarbons (PAHs). 41 UDP-glucuronosyltransferases (UGTs) and glutathione S -transferases (GSTs) are the major families of phase II enzymes, which could help binding of compounds to water-soluble groups and facilitate excretion. Indeed, genes encoding UGTs ( ugt-8 , ugt-13 , ugt-16 , ugt-40 , and ugt-41 ) and GSTs ( gst-14 , gst-21 , gst-30 , gst-35 , and gst-44 ) were up-regulated after exposure in this study.…”

Section: Discussionmentioning

confidence: 99%

β-Carbolines norharman and harman change neurobehavior causing neurological damage in Caenorhabditis elegans

Zhang,

Liu,

et al. 2023

Food Funct.

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“…UAF-1 is a large subunit of splicing factor U2AF (U2 auxiliary factor) involved in the recognition of 3' splice sites (Zorio and Blumenthal, 1999;Ma and Horvitz, 2009). IFBP-1 is an ortholog of human IRF2BP2 and IRF2BPL, which are members of the IRF2BP (interferon regulatory factor 2 binding protein) family of transcriptional repressors (Childs, 2003), while CYP-35A2 is a protein in Cytochrome P450 family (Lim et al, 2022). DDO-2 encodes D-aspartate oxidase that degrades D-amino acids (Saitoh et al, 2012).…”

Section: Fasting Upregulates Eif-2α Phosphorylation That Depends On G...mentioning

confidence: 99%

The integrated stress response protects against ER stress but is not required for altered translation and lifespan from dietary restriction in Caenorhabditis elegans

Ma,

Horrocks,

Mir

et al. 2023

Front. Cell Dev. Biol.

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The highly conserved integrated stress response (ISR) reduces and redirects mRNA translation in response to certain forms of stress and nutrient limitation. It is activated when kinases phosphorylate a key residue in the alpha subunit of eukaryotic translation initiation factor 2 (eIF2). General Control Nonderepressible-2 (GCN2) is activated to phosphorylate eIF2α by the presence of uncharged tRNA associated with nutrient scarcity, while protein kinase R-like ER kinase-1 (PERK) is activated during the ER unfolded protein response (UPRER). Here, we investigated the role of the ISR during nutrient limitation and ER stress with respect to changes in protein synthesis, translationally driven mRNA turnover, and survival in Caenorhabditis elegans. We found that, while GCN2 phosphorylates eIF2α when nutrients are restricted, the ability to phosphorylate eIF2α is not required for changes in translation, nonsense-mediated decay, or lifespan associated with dietary restriction (DR). Interestingly, loss of both GCN2 and PERK abolishes increased lifespan associated with dietary restriction, indicating the possibility of other substrates for these kinases. The ISR was not dispensable under ER stress conditions, as demonstrated by the requirement for PERK and eIF2α phosphorylation for decreased translation and wild type-like survival. Taken together, results indicate that the ISR is critical for ER stress and that other translation regulatory mechanisms are sufficient for increased lifespan under dietary restriction.

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CYP35 family in Caenorhabditis elegans biological processes: fatty acid synthesis, xenobiotic metabolism, and stress responses

Cited by 8 publications

References 89 publications

Orthosiphon aristatus (Blume) Miq Alleviates Non-Alcoholic Fatty Liver Disease via Antioxidant Activities in C57BL/6 Obese Mice and Palmitic–Oleic Acid-Induced Steatosis in HepG2 Cells

Orthosiphon aristatus (Blume) Miq Alleviates Non-Alcoholic Fatty Liver Disease via Antioxidant Activities in C57BL/6 Obese Mice and Palmitic–Oleic Acid-Induced Steatosis in HepG2 Cells

β-Carbolines norharman and harman change neurobehavior causing neurological damage in Caenorhabditis elegans

The integrated stress response protects against ER stress but is not required for altered translation and lifespan from dietary restriction in Caenorhabditis elegans

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