CYP311A1 in the anterior midgut is involved in lipid distribution and microvillus integrity in Drosophila melanogaster

Dong, Wei; Zhang, Xubo; Kong, Yong; Zhao, Zhenwen; Mahmoud, Ali; Wu, Lixian; Moussian, Bernard; Zhang, Jianzhen

doi:10.1007/s00018-022-04283-5

Cited by 4 publications

(12 citation statements)

References 42 publications

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“…By applying oil red O and BODIPY staining, we found that the lipid droplet numbers were reduced in wing discs of nub > Cyp311a1-RNAi flies. This Cyp311a1 knockdown effect resembles the phenotype found in the midgut (Dong et al, 2022). Thus, loss of function of Cyp311a1 affects lipid quantity in both the midgut and imaginal wing discs of D. melanogaster.…”

Section: Discussionsupporting

confidence: 58%

“…Depletion of Cyp311a1 in the larval midgut led to a reduction in the numbers of lipid droplets in the anterior midgut and the fat body. Larvae with reduced Cyp311a1 expression, by consequence, cease to grow (Dong et al, 2022). The main function of Cyp311a1 appears to be ensuring proper microvilli formation in the midgut where it is expressed and also, non-cell-autonomously, in other tissues such as the Malpighian tubules where it is absent.…”

Section: Discussionmentioning

confidence: 99%

“…We previously showed that Cyp311a1 is mainly expressed in the midgut. To determine whether Cyp311a1 is present in wing imaginal discs, we used our GFPtagged CYP311A1 version (Dong et al, 2022) to detect Cyp311a1 expression. A weak GFP signal was observed in wing discs of CYP311A1-GFP larvae, whereas no GFP signal was detected in wing discs of wild-type flies, indicating that Cyp311a1 is also expressed in wing discs (Fig.…”

Section: Expression Of Cyp311a1 In Wing Budsmentioning

confidence: 99%

“…In the anterior midgut, Cyp311a1 had previously been reported to be involved in lipid distribution (Dong et al, 2022). To further analyze wing phenotype after Cyp311a1 silencing, oil red O staining was performed in wing discs to detect lipid distribution.…”

Section: Suppression Of Cyp311a1 Leads To Reduction Of Lipid Amountsmentioning

confidence: 99%

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Role of CYP311A1 in wing development of Drosophila melanogaster

Zhang,

Liu,

Cheng

et al. 2024

Insect Science

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Lipid homeostasis is crucial for growth and development of organisms. Several cytochrome P450 monooxygenases (CYPs) are involved in lipid metabolism. The function of Cyp311a1 in the anterior midgut as a regulator of phosphatidylethanolamine (PE) metabolism in Drosophila melanogaster has been demonstrated, as depletion of Cyp311a1 caused larval growth arrest that was partially rescued by supplying PE. In this study, we investigated the role of CYP311A1 in wing morphogenesis in Drosophila. Using the GAL4‐UAS system, Cyp311a1 was selectively knocked down in the wing disc. A deformed wing phenotype was observed in flies with reduced Cyp311a1 transcripts. BODIPY and oil red O staining revealed a reduction of neutral lipids in the wing disc after the depletion of Cyp311a1. In addition, we observed an enhanced sensitivity to Eosin Y penetration in the wings of Cyp311a1 knocked‐down flies. Moreover, the reduction of CYP311A1 function in developing wings does not affect cell proliferation and apoptosis, but entails disordered Phalloidin or Cadherin distribution, suggesting an abnormal cell morphology and cell cortex structure in wing epithelial cells. Taken together, our results suggest that Cyp311a1 is needed for wing morphogenesis by participating in lipid assembly and cell homeostasis.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Expression Of Cyp311a1 In Wing Budsmentioning

confidence: 99%

Section: Suppression Of Cyp311a1 Leads To Reduction Of Lipid Amountsmentioning

confidence: 99%

See 2 more Smart Citations

Role of CYP311A1 in wing development of Drosophila melanogaster

Zhang,

Liu,

Cheng

et al. 2024

Insect Science

Self Cite

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“…Analysis of paraffin sections of the midgut showed that the intestinal wall was thinner in the 980A, 980N and Anti‐Atg2B groups than in the control group (Figure 5a). The thickness of the intestinal wall is closely related to its digestive and absorptive capacity and intestinal microvilli allow more absorption to occur by increasing the surface area of intestinal cells (Baumann, 2001; Dong et al, 2022). To assess the effect of ame‐miR‐980‐3p on midgut development in a more systematic and comprehensive manner, microvilli of the midgut of NE were analysed by transmission electron microscopy (TEM) in the C, 980A and 980N groups.…”

Section: Resultsmentioning

confidence: 99%

Ame‐miR‐980‐3p participates in autophagy‐mediated midgut remodelling in Apis mellifera via targeting Atg2B

Chen,

Chi,

Song

et al. 2023

Insect Molecular Biology

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Autophagy is a process that serves to degrade damaged proteins and organelles, thereby promoting cell homeostasis, differentiation, development and survival. Many miRNAs have been found to have regulatory roles in autophagy. In insects, it has been shown that autophagy is involved in hormone‐regulated programmed cell death during metamorphic midgut remodelling. However, whether this is also true during the remodelling of the honey bee midgut is unclear. In the present study, we explored the relationship between autophagy and midgut remodelling and sought to identify miRNAs involved in this physiological process. We found that autophagy occurred during midgut remodelling and that the inhibition of autophagy resulted in midgut dysplasia in prepupae. Differentially expressed miRNAs enriched in the autophagy signalling pathway during midgut remodelling were identified by small RNA‐seq. Ame‐miR‐980‐3p, which targets the autophagy‐related gene Atg2B, was screened out. Furthermore, abnormal expression of ame‐miR‐980‐3p in the pupal stage led to the thinning of the midgut wall of newly emerged bees (NE). When ame‐miR‐980‐3p expression was inhibited, the intestinal villi of NE bees became significantly shorter and sparse, and the lipid signal in the peritrophic matrix of Pb almost disappeared, indicating that the adult midgut was underdeveloped and the lipid absorption ability was weakened. Taken together, ame‐miR‐980‐3p targeted Atg2B to participate in the regulation of midgut autophagy in the pupae, and the abnormal expression of ame‐miR‐980‐3p would interfere with cell proliferation and death in the process of midgut remodelling, hinder the formation of adult midgut and eventually lead to adult midgut dysplasia and affect the lipid absorption function of the midgut in Apis mellifera.

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Characterization of the TcCYPE2 Gene and Its Role in Regulating Trehalose Metabolism in Response to High CO2 Stress

Zhou,

Wang

et al. 2023

Agronomy

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Cytochrome P450 monooxygenase (CYP) is one of the three detoxification metabolic enzymes in insects, and is involved in the metabolism and transformation of endogenous substances as well as the activation and degradation of exogenous compounds. This study aims to reveal the molecular mechanism of CYP9E2 in Tribolium castaneum in adapting to high-CO2 stress. By predicting the sequence function of CYP9E2, analyzing the temporal and spatial expression profile of TcCYP9E2, and using RNAi to silence TcCYP9E2 combined with a high-CO2 stress treatment, we measured the carbohydrate content, trehalase activity, and gene expression levels in trehalose metabolism of T. castaneum. A bioinformatics analysis showed that the predicted molecular weight of the protein encoded by TcCYP9E2 is 60.15, the theoretical isoelectric point is 8.63, there is no signal peptide, and the protein is hydrophilic. An evolutionary tree analysis showed that TcCYP9E2 belongs to the CYP6 family and belongs to the CYP3 group; and the spatiotemporal expression profile results showed that TcCYP9E2 was highly expressed in the larvae midgut 48 h after injection of dsCYP9E2, with survival rates decreasing with the increase in CO2 concentration. Under the condition of 75% CO2, the contents of glycogen, glucose, ATP, and membrane-bound trehalase decreased significantly after the injection of dsCYP9E2. The expression of TRE-1, TRE-2, and GP in trehalose metabolism and energy pathways was significantly downregulated.

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CYP311A1 in the anterior midgut is involved in lipid distribution and microvillus integrity in Drosophila melanogaster

Cited by 4 publications

References 42 publications

Role of CYP311A1 in wing development of Drosophila melanogaster

Role of CYP311A1 in wing development of Drosophila melanogaster

Ame‐miR‐980‐3p participates in autophagy‐mediated midgut remodelling in Apis mellifera via targeting Atg2B

Characterization of the TcCYPE2 Gene and Its Role in Regulating Trehalose Metabolism in Response to High CO2 Stress

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