CYP1A1 protein and mRNA in teleosts as an environmental bioindicator: laboratory and environmental studies

Haasch, Mary L.; Quardokus, Ellen M.; Sutherland, Leslie A.; Goodrich, Mark S.; Prince, Ruth; Cooper, Keith; Lech, John J.

doi:10.1016/0141-1136(92)90098-7

Cited by 29 publications

(10 citation statements)

References 10 publications

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“…Nyholm (1992) noted that there is a better definition of exposure time, and it is possible to study an impacted zone surrounding a relatively precise outlet. For field caging, the time of exposure reported in the literature varied from 1 week (Haasch et al, 1992) to 3 weeks (Lindstro¨m-Seppa¨and Oikari, 1989). A previous study (Fenet et al, 1996) reported that EROD activity stabilized within 1 week when European eels were transferred from the field to laboratory tanks.…”

Section: Discussionmentioning

confidence: 97%

“…Direct immersion of fish in precise sites (polluted sites and reference sites) allows better control of the conditions of exposure to toxicants present in the aquatic environment. This can be accomplished by fish caging (Lindstro¨m-Seppa¨and Oikari, 1989;Haasch et al, 1992). Different species have been used during field-caging studies; however, the response of hepatic enzymatic activities such as EROD activity is variable within species (Goks+yr, 1995, Collier et al, 1995.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Laboratory and Field-Caging Studies on Hepatic Enzymatic Activities in European Eel and Rainbow Trout

Fenet¹,

Casellas²,

Bontoux³

1998

Ecotoxicology and Environmental Safety

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Laboratory and Field-Caging Studies on Hepatic Enzymatic Activities in European Eel and Rainbow Trout

Fenet¹,

Casellas²,

Bontoux³

1998

Ecotoxicology and Environmental Safety

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“…The enzymatic activity, however, was often under the influence of various species of drug-metabolizing enzymes, making it difficult to detect high inducibility of a specific gene responsible for a pollutant. As the fish CYP1A complementary DNA sequences have been reported for several species (Leaver et al, 1993;Berndtson and Chen, 1994;Mizukami et al, 1994;Morrison et al, 1995;Roy et al, 1995), measurement of transcribed RNA provides a method that is specific and highly sensitive to expression of one gene (Haasch et al, 1992;Campbell and Devlin, 1996). However, only a few studies on the regulation of CYP1A gene expression in fish have been reported (Pesonen et al, 1992;Hahn and Stegeman, 1994).…”

Section: Introductionmentioning

confidence: 99%

Induction of Two Forms of Eel Cytochrome P450 1A Genes by 3-Methylcholanthrene

et al. 1999

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: In eel (Anguilla japonica), exposure to polyaromatic hydrocarbons such as 3-methylcholanthrene leads to induction of two CYP1A enzymes, CYP1A1 and CYP1A6. We studied the time course and tissue specificity of induction of messenger RNAs for CYP1A1 and CYP1A6 in eel by administering 3-methylcholanthrene intraperitoneally. In both cases, the drug induced a rapid increase of mRNAs and biphasic expression. In the liver, mRNA levels of CYP1A1 and CYP1A6 increased 22-fold at 3 hours and 27-fold at 6 hours after the administration, respectively, showing initial peaks in the induction. After the initial inductions, mRNA levels decreased unexpectedly. Following these temporary decreases, the mRNA levels again increased and reached levels that were 35 and 41 times the basal levels at 24 hours after administration, respectively. CYP1A1 and CYP1A6 resembled each other also in the tissue specificity of gene expression; the expression levels were liver >> gill > intestine > kidney. The rapid induction, the biphasic expression, and the tissue-specific expression were common features of gene expression in CYP1A1 and CYP1A6 and may come from common structures of the regulatory regions of the two genes.

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“…The enzymatic activity, however, was often under the influence of various species of drug-metabolizing enzymes, making it difficult to detect high inducibility of a specific gene responsible for a pollutant. Measurement of transcribed RNA provides a method that is specific and highly sensitive to expression of one gene (Haasch et al, 1992;Pesonen et al, 1992;Hahn and Stegeman, 1994;Campbell and Devlin, 1996).…”

Section: Introductionmentioning

confidence: 99%

Functional Analysis of Promoter Region from Eel Cytochrome P450 1A1 Gene in Transgenic Medaka

et al. 1999

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: Transcription of the CYP1A1 genes in mammals and fish is stimulated by polyaromatic hydrocarbons. DNA sequencing analysis revealed that CYP1A1 gene in eel (Anguilla japonica) contains two kinds of putative cis-acting regulatory elements, XRE (xenobiotic-responsive element) and ERE (estrogen-responsive element). XRE is known as the enhancer that is responsible for the inducibility of the genes of CYP1A1 and some other drug-metabolizing enzymes. In the eel CYP1A1 gene, XRE motifs are distributed as follows: five times in the region from -2136 to -1125 bp, XRE(-6) to (-2); once in the proximal basal promoter region, XRE(-1); and once in the first intron, XRE(+1). The region between XRE(-2) and XRE(-1) contains three ERE motifs. To investigate the function of the cis-acting regulatory elements in the eel CYP1A1 gene, recombinant plasmids prepared with its 5' upstream sequence and the structural gene for luciferase were microinjected into fertilized eggs of medaka at the one-cell stage. Hatched fry were treated with 3-methylcholanthrene, and the transcription efficiency was assayed using competitive polymerase chain reaction analysis. Deletion of the region containing the five XREs, XRE(-6) to XRE(-2), and the point mutation of XRE(-1) reduced the inducible expressions by 75% and 56%, respectively, showing apparent dependency of the drug induction on the XREs. Constitutive expression, however, was not significantly affected by deletion or disruption of the XREs. When the region between XRE(-2) and XRE(-1) containing no XREs but three ERE motifs was internally deleted, the inducible expression and the constitutive expression were reduced by 88% and 75%, respectively. Replacement of this region with a partial fragment of eel CYP1A1 complementary DNA, with slight alteration of the distance between the five XREs and XRE(-1), reduced the inducible expression and the constitutive expression by 91% and 60%, respectively. These results strongly suggest that not only XRE but also other regulatory elements, possibly ERE, play an important role in induced and constitutive expressions of the eel CYP1A1 gene.

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CYP1A1 protein and mRNA in teleosts as an environmental bioindicator: laboratory and environmental studies

Cited by 29 publications

References 10 publications

Laboratory and Field-Caging Studies on Hepatic Enzymatic Activities in European Eel and Rainbow Trout

Laboratory and Field-Caging Studies on Hepatic Enzymatic Activities in European Eel and Rainbow Trout

Induction of Two Forms of Eel Cytochrome P450 1A Genes by 3-Methylcholanthrene

Functional Analysis of Promoter Region from Eel Cytochrome P450 1A1 Gene in Transgenic Medaka

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