CYP19A1 May Influence Lambing Traits in Goats by Regulating the Biological Function of Granulosa Cells

Zhang, Yan; Chen, Xiang; Zhou, Zhinan; Tian, Xingzhou; Yang, Ping; Fu, Kaibin

doi:10.3390/ani12151911

Cited by 8 publications

(5 citation statements)

References 47 publications

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“…9 ). This result was consistent with the report of Zhang et al in goat GCs [ 39 ]. In addition, Zhao et al demonstrated in a mouse model that PAK1 mediates a negative regulation of E 2 in the non-classical ERα pathway in the reproductive axis, and also confirmed that PAK1 inhibits E 2 secretion in this study, thereby inhibiting follicular development and ovulation, thereby regulating sheep reproduction [ 40 ].…”

Section: Discussionsupporting

confidence: 94%

Whole-genome resequencing of the native sheep provides insights into the microevolution and identifies genes associated with reproduction traits

Zhu

Yang

et al. 2023

BMC Genomics

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Background Sheep genomes undergo numerous genes losses, gains and mutation that generates genome variability among breeds of the same species after long time natural and artificial selection. However, the microevolution of native sheep in northwest China remains elusive. Our aim was to compare the genomes and relevant reproductive traits of four sheep breeds from different climatic environments, to unveil the selection challenges that this species cope with, and the microevolutionary differences in sheep genomes. Here, we resequenced the genomes of 4 representative sheep breeds in northwest China, including Kazakh sheep and Duolang sheep of native breeds, and Hu sheep and Suffolk sheep of exotic breeds with different reproductive characteristics. Results We found that these four breeds had a similar expansion experience from ~ 10,000 to 1,000,000 years ago. In the past 10,000 years, the selection intensity of the four breeds was inconsistent, resulting in differences in reproductive traits. We explored the sheep variome and selection signatures by FST and θπ. The genomic regions containing genes associated with different reproductive traits that may be potential targets for breeding and selection were detected. Furthermore, non-synonymous mutations in a set of plausible candidate genes and significant differences in their allele frequency distributions across breeds with different reproductive characteristics were found. We identified PAK1, CYP19A1 and PER1 as a likely causal gene for seasonal reproduction in native sheep through qPCR, Western blot and ELISA analyses. Also, the haplotype frequencies of 3 tested gene regions related to reproduction were significantly different among four sheep breeds. Conclusions Our results provide insights into the microevolution of native sheep and valuable genomic information for identifying genes associated with important reproductive traits in sheep.

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Section: Discussionsupporting

confidence: 94%

Whole-genome resequencing of the native sheep provides insights into the microevolution and identifies genes associated with reproduction traits

Zhu

Yang

et al. 2023

BMC Genomics

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“…Wang et al noted that GLOD4 expression was significantly upregulated in the testis of Tibetan sheep wwith age, and GLOD4 was only expressed in leydig cells and that GLOD4 was mainly observed in the cytoplasm [23], our immunohistochemistry revealed that GLOD4 was expressed only in leydig cells, which is consistent with the findings of Wang et al However, our subcellular localization analysis revealed that GLOD4 was widely expressed in both the nucleus and the cytoplasm of leydig cells. Interestingly, in a recent study, fluorescently tagged GLOD4 protein was widely distributed in the cytoplasm and nucleus of human retinal pigment epithelial cells (hTERT-RPE1) [28], which may result from differential gene expression in different species [29][30][31].…”

Section: Discussionsupporting

confidence: 90%

“…As a goat breed that is well adapted to the highland mountain environment, Qianbei Ma goats have a long history of breeding in Guizhouwith a medium body size, rough feeding resistance, strong disease resistance, good fur quality and strong fertility. Thus, Qianbei Ma goats are a good model animal for exploring the reproductive traits of plateau goats [23]. Therefore, the aim of this study was to investigate the profile of GLOD4 gene expression in the testes of goats at different ages and to analyze the regulatory effect of the GLOD4 gene on testicular leydig cells.…”

Section: Introductionmentioning

confidence: 99%

Expression of GLOD4 Gene in the Testis of Qianbei Ma Goats and Its Effect on Testicular Leydig Cells

Wang,

Chen,

Sun

et al. 2024

Preprint

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The objective of this paper was to study the expression pattern of the GLOD4 gene in the testis and its regulatory effect on testicular cells. Currently, the regulated mechanism through are unclear. Here, we explored how GLOD4 functions in goat leydig cells to enhance our understanding of spermatogenesis and improve reproduction in breeding rams. In this study, we demonstrated that the localization of GLOD4 in testicular cells using immunohistochemistry and subcellular localization analyses. Subsequently, we analyzed the GLOD4 expression pattern in 4 age-based groups (0, 6, 12, and 18 months old) using real-time quantitative polymerase chain reaction (qRT‒PCR) and protein blotting. Finally, we performed GLOD4 gene silencing and overexpression studies in testicular cells from goats and explored the effects of GLOD4 on cell proliferation, the cell cycle, steroid hormone secretion and the expression of candidate testosterone hormone-regulated genes. GLOD4 was mainly expressed in testicular leydig cells, and the subcellular localization results showed that the GLOD4 protein was mainly localized in the cytoplasm and nucleus. Silencing of GLOD4 significantly suppressed the mRNA expression levels of the testosterone secretion-related genes CYP11A1, 3β-HSD and CYP17A1 and the mRNA expression levels of cell-cycle related genes CDK6, PCNA, and Cyclin E. Moreover, the cell cycle was blocked at the G2/M phase after GLOD4 silencing, which significantly suppressed testosterone secretion. In contrast, GLOD4 overexpression significantly increased the mRNA expression levels of the testosterone secretion-related genes CYP11A1, 3β-HSD, and CYP17A1 and increased the expression of the cell-cycle related genes CDK6, PCNA, and Cyclin E. Moreover, GLOD4 overexpression promoted the cell cycle from G0/G1 phases to enter the S phase and G2/M phases,promoted the secretion of testosterone. Taken together, our experimental results indicate that GLOD4 may affect the development of cells in Qianbei Ma goats of different ages by influencing the cell cycle, Proliferation and testosterone hormone synthesis. These findings enhance our understanding of the functions of GLOD4 in the cells of goats.

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“…It is known that CYP19A1 is widely expressed in ovaries and granulosa cells, where it converts androgens into estrogens. Importantly, CYP19A1 has been demonstrated to influence lambing traits in goats by affecting granulosa cell proliferation, hormone secretion, and the expression of candidate genes associated with traits for multiple births [10,37]. GHRHR, a member of the superfamily of the G-protein-coupled receptor subfamily, works together with the growth-hormone-releasing hormone (GHRH) to regulate the growth hormone axis and the development and proliferation of pituitary somatotropes.…”

Section: Discussionmentioning

confidence: 99%

Characterization and Comparative Analysis of Whole-Transcriptome Sequencing in High- and Low-Fecundity Chongming White Goat Ovaries during the Estrus Phase

Lin,

Sun,

Dai

et al. 2024

Animals

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Reproductive performance is one of the most important economic traits in the goat industry. Increasing the number of goats is an effective measure to improve production efficiency and reduce production costs. Ovaries are important reproductive organs in female mammals that directly affect the estrous cycle and reproductive abilities. Understanding the complex transcription network of non-coding RNAs (lncRNAs, circRNAs, and miRNAs) and messenger RNA (mRNA) could lead to significant insights into the ovarian regulation of the reproductive processes of animals. However, the whole-transcriptome analysis of the non-coding RNAs and mRNA of the ovaries in Chongming white goats between high-fecundity (HP) and low-fecundity (LP) groups is limited. In this study, a whole-transcriptome sequencing approach was used to identify lncRNA, circRNA, miRNA, and mRNA expression in the ovaries of Chongming white goats during the estrus phase using RNA-Seq technology. More than 20,000 messenger RNAs (mRNAs), 10,000 long non-coding RNAs (lncRNAs), 3500 circular RNAs (circRNAs), and 1000 micro RNAs (miRNAs) were identified. A total of 1024 differential transcripts (724 mRNAs, 112 lncRNAs, 178 circRNAs, and 10 miRNAs) existing between the HP and the LP groups were revealed through a bioinformatics analysis. They were enriched in the prolactin signaling pathway, the Jak–STAT signaling pathway, and the GnRH signaling pathway, as well as various metabolic pathways. Differentially expressed mRNAs (such as LYPD6, VEGFA, NOS3, TNXB, and EPHA2) and miRNAs (such as miR-10a-5p) play key roles in the regulation of goat ovaries during the estrus phase. The enrichment of pathways related to reproduction, such as the Hippo, Hedgehog, PI3K–AKT, and MAPK signaling pathways, suggests that they might be involved in the prolificacy of goat ovaries. Overall, we identified several gene modules associated with goat fecundity and provided a basis for a molecular mechanism in the ovaries of Chongming white goats.

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CYP19A1 May Influence Lambing Traits in Goats by Regulating the Biological Function of Granulosa Cells

Cited by 8 publications

References 47 publications

Whole-genome resequencing of the native sheep provides insights into the microevolution and identifies genes associated with reproduction traits

Whole-genome resequencing of the native sheep provides insights into the microevolution and identifies genes associated with reproduction traits

Expression of GLOD4 Gene in the Testis of Qianbei Ma Goats and Its Effect on Testicular Leydig Cells

Characterization and Comparative Analysis of Whole-Transcriptome Sequencing in High- and Low-Fecundity Chongming White Goat Ovaries during the Estrus Phase

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