CYP1-mediated antiproliferative activity of dietary flavonoids in MDA-MB-468 breast cancer cells

Androutsopoulos, Vasilis P.; Ruparelia, Ketan C.; Arroo, R. R. J.; Tsatsakis, Aristidis; Spandidos, Demetrios Α.

doi:10.1016/j.tox.2009.07.023

Cited by 84 publications

(82 citation statements)

References 44 publications

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“…39 The anticancer effects of flavonoids occur through oxidative destruction, inhibition of proliferation, inactivation of carcinogen, promotion of differentiation, induction of cell-cycle arrest and apoptosis, impairment of tumor angiogenesis, and suppression of metastasis. [40][41][42] Flavonoids can interact with xenobiotic metabolizing enzymes and inhibit involvement of kinases signal transduction, interact with estrogen type II binding sites, and alter gene expression patterns, 43,44 with resultant promotion of antiproliferative activity of Pd@W.tea NPs.…”

Section: In Vitro Cytotoxicitymentioning

confidence: 99%

Green synthesis palladium nanoparticles mediated by white tea (<em>Camellia sinensis</em>) extract with antioxidant, antibacterial, and antiproliferative activities toward the human leukemia (MOLT-4) cell line

Azizi¹,

Shahri²,

Rahman³

et al. 2017

IJN

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Among nanoparticles used for medical applications, palladium nanoparticles (PdNPs) are among the least investigated. This study was undertaken to develop PdNPs by green synthesis using white tea (W.tea; Camellia sinensis ) extract to produce the Pd@W.tea NPs. The Pd@W.tea NPs were characterized by UV–vis spectroscopy and X-ray diffractometry, and evaluated with transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The Pd@W.tea NPs were spherical (size 6–18 nm) and contained phenols and flavonoids acquired from the W.tea extract. Pd@W.tea NPs has good 1-diphenyl-2-picrylhydrazyl (DPPH), OH, and NO-scavenging properties as well as antibacterial effects toward Staphylococcus epidermidis and Escherichia coli . MTT assay showed that Pd@W.tea NPs (IC 50 =0.006 μM) were more antiproliferative toward the human leukemia (MOLT-4) cells than the W.tea extract (IC 50 =0.894 μM), doxorubicin (IC 50 =2.133 μM), or cisplatin (IC 50 =0.013 μM), whereas they were relatively innocuous for normal human fibroblast (HDF-a) cells. The anticancer cell effects of Pd@W.tea NPs are mediated through the induction of apoptosis and G2/M cell-cycle arrest.

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Section: In Vitro Cytotoxicitymentioning

confidence: 99%

Green synthesis palladium nanoparticles mediated by white tea (<em>Camellia sinensis</em>) extract with antioxidant, antibacterial, and antiproliferative activities toward the human leukemia (MOLT-4) cell line

Azizi¹,

Shahri²,

Rahman³

et al. 2017

IJN

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“…Therapeutic agents include doxorubicin which has known cardiotoxic activity, 38 apigenin which has known cardioprotective activity 39 and luteolin which is a known active metabolite of apigenin. 40 The drug concentration (100 lM) was established from a short incubation (4 h, minimum time to allow for cell adhesion) cytotoxicity curve (data not shown) for apigenin (the least active of the three compounds) in order to identify a concentration that would elicit marginal cell toxicity (IC 25 ) presonication. This concentration was then applied with luteolin and doxorubicin in order to observe whether CA-free sonoporation was achievable for structurally diverse drug molecules, regardless of their initial or intrinsic toxicity.…”

Section: Drug Cytotoxicity Comparison Pre-and Post-sonicationsmentioning

confidence: 99%

Contrast agent-free sonoporation: The use of an ultrasonic standing wave microfluidic system for the delivery of pharmaceutical agents

et al. 2011

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Sonoporation is a useful biophysical mechanism for facilitating the transmembrane delivery of therapeutic agents from the extracellular to the intracellular milieu. Conventionally, sonoporation is carried out in the presence of ultrasound contrast agents, which are known to greatly enhance transient poration of biological cell membranes. However, in vivo contrast agents have been observed to induce capillary rupture and haemorrhage due to endothelial cell damage and to greatly increase the potential for cell lysis in vitro. Here, we demonstrate sonoporation of cardiac myoblasts in the absence of contrast agent (CA-free sonoporation) using a low-cost ultrasound-microfluidic device. Within this device an ultrasonic standing wave was generated, allowing control over the position of the cells and the strength of the acoustic radiation forces. Real-time single-cell analysis and retrospective postsonication analysis of insonated cardiac myoblasts showed that CA-free sonoporation induced transmembrane transfer of fluorescent probes (CMFDA and FITC-dextran) and that different mechanisms potentially contribute to membrane poration in the presence of an ultrasonic wave. Additionally, to the best of our knowledge, we have shown for the first time that sonoporation induces increased cell cytotoxicity as a consequence of CA-free ultrasound-facilitated uptake of pharmaceutical agents (doxorubicin, luteolin, and apigenin). The US-microfluidic device designed here provides an in vitro alternative to expensive and controversial in vivo models used for early stage drug discovery, and drug delivery programs and toxicity measurements.

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“…[17][18][19] It has been postulated that dietary flavonoids exhibit cancer therapeutic effects, due to intracellular CYP1-mediated metabolism to conversion products that inhibit growth of cancerous cells. [16][17][18][19][20] Thus the duality of flavonoids in cancer therapy and prevention is determined by both, their substrate and inhibitor propensities towards CYP1A1 and CYP1B1 enzymes. 16 Inhibition of 7-ethoxyresorufin-O-deethylase activity (EROD) is a well documented assay used predominantly to test the inhibitory potencies of dietary flavonoids.…”

Section: Introductionmentioning

confidence: 99%

Comparative CYP1A1 and CYP1B1 substrate and inhibitor profile of dietary flavonoids

Androutsopoulos

Papakyriakou

Vourloumis

et al. 2011

Bioorganic & Medicinal Chemistry

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CYP1-mediated antiproliferative activity of dietary flavonoids in MDA-MB-468 breast cancer cells

Cited by 84 publications

References 44 publications

Green synthesis palladium nanoparticles mediated by white tea (<em>Camellia sinensis</em>) extract with antioxidant, antibacterial, and antiproliferative activities toward the human leukemia (MOLT-4) cell line

Green synthesis palladium nanoparticles mediated by white tea (<em>Camellia sinensis</em>) extract with antioxidant, antibacterial, and antiproliferative activities toward the human leukemia (MOLT-4) cell line

Contrast agent-free sonoporation: The use of an ultrasonic standing wave microfluidic system for the delivery of pharmaceutical agents

Comparative CYP1A1 and CYP1B1 substrate and inhibitor profile of dietary flavonoids

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