Cylindrospermopsin impairs zebrafish (Danio rerio) embryo development

Moraes, A.C.N.; Shah, Sophia; Magalhães, Valéria Freitas de; Habibi, Hamid R.

doi:10.1016/j.marenvres.2022.105567

Cited by 6 publications

(4 citation statements)

References 50 publications

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“…In addition, CYN induced changes in thyroid-hormonerelated genes (tr1a, Dio1, Dio3) which could be involved in the induction of thyroid disrup-tion. Globally, the authors indicated that CYN exposure alters functions related to thyroid hormones, oxidative stress, and osmoregulation, all of them key components for normal embryo development [116]. These results confirmed the adverse effects on embryonic development induced by CYN in zebrafish exposed to the toxin (2-2000 nM) previously reported by Wang et al [117], who indicated that they may be associated with oxidative stress and apoptosis.…”

Section: Cylindrospermopsinsupporting

confidence: 80%

“…Conversely, pure CYN did show binding affinity to the estrogen receptor [113]. Although studies dealing with CYN are very limited, and the results obtained were also variable, it showed ED effects mediated by changes in the transcript levels of related hormones, apoptosis induction [114], changes in thyroid-hormone-related genes [116], oxidative stress [117], or alterations in hormone levels [114,115].…”

Section: General Discussion and Final Remarksmentioning

confidence: 99%

“…The same authors investigated the adverse effects of CYN (0-2000 µg/L CYN) on zebrafish embryonic development [116], and numerous changes were observed: decreased growth, increased developmental abnormalities, such as pericardial and yolk sac edema, as well as swim bladder absence. In addition, CYN induced changes in thyroid-hormonerelated genes (tr1a, Dio1, Dio3) which could be involved in the induction of thyroid disrup-tion.…”

Section: Cylindrospermopsinmentioning

confidence: 99%

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Potential Endocrine Disruption of Cyanobacterial Toxins, Microcystins and Cylindrospermopsin: A Review

Casas-Rodríguez

Cameán

Jos

2022

Toxins

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Microcystins (MCs) and cylindrospermopsin (CYN), although classified as hepatotoxins and cytotoxins, respectively, have been shown to also induce toxic effects in many other systems and organs. Among them, their potential endocrine disruption (ED) activity has been scarcely investigated. Considering the increasing relevance of ED on humans, mammals, and aquatic organisms, this work aimed to review the state-of-the-art regarding the toxic effects of MCs and CYN at this level. It has been evidenced that MCs have been more extensively investigated than CYN. Reported results are contradictory, with the presence or absence of effects, but experimental conditions also vary to a great extent. In general, both toxins have shown ED activity mediated by very different mechanisms, such as estrogenic responses via a binding estrogen receptor (ER), pathological changes in several organs and cells (testis, ovarian cells), and a decreased gonad-somatic index. Moreover, toxic effects mediated by reactive oxygen species (ROS), changes in transcriptional responses on several endocrine axes and steroidogenesis-related genes, and changes in hormone levels have also been reported. Further research is required in a risk assessment frame because official protocols for assessment of endocrine disrupters have not been used. Moreover, the use of advanced techniques would aid in deciphering cyanotoxins dose-response relationships in relation to their ED potential.

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Section: Cylindrospermopsinsupporting

confidence: 80%

Section: General Discussion and Final Remarksmentioning

confidence: 99%

Section: Cylindrospermopsinmentioning

confidence: 99%

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Potential Endocrine Disruption of Cyanobacterial Toxins, Microcystins and Cylindrospermopsin: A Review

Casas-Rodríguez

Cameán

Jos

2022

Toxins

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“…In particular, the 70 kDa-HSP (Hsp70) family promotes the proteolytic removal of oxidatively damaged proteins by the proteasome ( 18 ) and closely interact with the nitric oxide generation systems ( 19 ). Hsp70 is represents a marker of oxidative response in different experimental models, including Danio rerio (commonly called zebrafish) ( 20 – 22 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of carnosine on the embryonic development and TiO2 nanoparticles-induced oxidative stress on Zebrafish

Caruso

Scalisi²,

Pecoraro³

et al. 2023

Front. Vet. Sci.

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Oxidative stress is due to an unbalance between pro-oxidants, such as reactive oxygen (ROS) and nitrogen (RNS) species, and antioxidants/antioxidant system. Under physiological conditions these species are involved in different cellular processes such as cellular homeostasis and immune response, while an excessive production of ROS/RNS has been linked to the development of various diseases such as cancer, diabetes, and Alzheimer's disease. In this context, the naturally occurring dipeptide carnosine has shown the ability to scavenge ROS, counteract lipid peroxidation, and inhibit proteins oxidation. Titanium dioxide nanoparticles (TiO2-NPs) have been widely used to produce cosmetics, in wastewater treatment, in food industry, and in healthcare product. As consequence, these NPs are often released into aquatic environments. The Danio rerio (commonly called zebrafish) embryos exposure to TiO2-NPs did not affect the hatching rate, but induced oxidative stress. According to this scenario, in the present study, we first investigated the effects of carnosine exposure and of a sub-toxic administration of TiO2-NPs on the development and survival of zebrafish embryos/larvae measured through the acute embryo toxicity test (FET-Test). Zebrafish larvae represent a useful model to study oxidative stress-linked disorders and to test antioxidant molecules, while carnosine was selected based on its well-known multimodal mechanism of action that includes a strong antioxidant activity. Once the basal effects of carnosine were assessed, we then evaluated its effects on TiO2-NPs-induced oxidative stress in zebrafish larvae, measured in terms of total ROS production (measured with 2,7-dichlorodihydrofluorescein diacetate probe) and protein expression by immunohistochemistry of two cellular stress markers, 70 kDa-heat shock protein (Hsp70) and metallothioneins (MTs). We demonstrated that carnosine did not alter the phenotypes of both embryos and larvae of zebrafish at different hours post fertilization. Carnosine was instead able to significantly decrease the enhancement of ROS levels in zebrafish larvae exposed to TiO2-NPs and its antioxidant effect was paralleled by the rescue of the protein expression levels of Hsp70 and MTs. Our results suggest a therapeutic potential of carnosine as a new pharmacological tool in the context of pathologies characterized by oxidative stress such as neurodegenerative disorders.

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From benthic to floating: phytoplankton dynamics in African freshwater lakes and reservoirs

Madzivanzira¹,

Mungenge²,

Dube³

et al. 2023

Lakes of Africa

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Cylindrospermopsin impairs zebrafish (Danio rerio) embryo development

Cited by 6 publications

References 50 publications

Potential Endocrine Disruption of Cyanobacterial Toxins, Microcystins and Cylindrospermopsin: A Review

Potential Endocrine Disruption of Cyanobacterial Toxins, Microcystins and Cylindrospermopsin: A Review

Effects of carnosine on the embryonic development and TiO2 nanoparticles-induced oxidative stress on Zebrafish

From benthic to floating: phytoplankton dynamics in African freshwater lakes and reservoirs

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