CyHV-2 ORF104 activates the p38 MAPK pathway

Du, Mi; Chen, Mingliang; Shen, Haifeng; Wang, Wei; Wang, Weiyi; Huang, Jianhui; Chen, Jianming

doi:10.1016/j.fsi.2015.06.011

Cited by 12 publications

(7 citation statements)

References 36 publications

(26 reference statements)

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“…The mitogen-activated protein kinase (MAPK) signal transduction pathway transfers signals from the cell surface to the cell interior for the regulation of cell growth and death. Cell fate is tightly regulated by the MAPK signaling pathway under various stressed conditions such as endoplasmic reticulum (ER) stress, hypoxia, and inflammation (Du et al, 2015;Farrukh et al, 2015). ERK, JNK, and p38 are three MAPK subgroups activated in response to ER stress (Darling and Cook, 2014).…”

Section: Erk/mapk Signaling Pathwaymentioning

confidence: 99%

Natural Product Ginsenoside 20(S)-25-Methoxyl-Dammarane-3β, 12β, 20-Triol in Cancer Treatment: A Review of the Pharmacological Mechanisms and Pharmacokinetics

et al. 2020

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Panax ginseng has been used as an herbal medicine for thousands of years. Most of its pharmacological effects are attributed to its constituent ginsenosides, including 20(S)-25methoxyl-dammarane-3b, 12b, 20-triol (20(S)-25-OCH 3 -PPD), which is one of the protopanaxadiol type ginsenosides. It has been found to exhibit anticancer effects by interacting with multiple pharmacological pathways, such as the Wnt/b-catenin, MDM2, ERK/MAPK, and STAT3 signaling pathways. However, its therapeutic potential could be limited by its low bioavailability mainly due to its low aqueous solubility. Thus, several studies have been conducted on its pharmacokinetics and its delivery systems, so as to increase its oral bioavailability. In this review, comprehensive information on its varying pharmacological pathways in cancer, as well as its pharmacokinetic behavior and pharmaceutical strategies, is provided. This information would be useful in the understanding of its diverse mechanisms and pharmacokinetics as an anticancer drug, leading to the design of superior 20(S)-25-OCH 3 -PPD-containing formulations that maximize its therapeutic potential.

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Section: Erk/mapk Signaling Pathwaymentioning

confidence: 99%

Natural Product Ginsenoside 20(S)-25-Methoxyl-Dammarane-3β, 12β, 20-Triol in Cancer Treatment: A Review of the Pharmacological Mechanisms and Pharmacokinetics

et al. 2020

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“…Bioinformatics analysis indicated 154 predicted open reading frames (ORFs) in the genome of CyHV-2, but the functions of most genes are unknown ( 2 ). A previous study demonstrated that ORF104 of CyHV-2 encodes a protein kinase, which can activate the p38-MAPK signaling pathway during CyHV-2 infection, and inhibiting this pathway could reduce the lethality of CyHV-2 ( 8 ). Although some progress has been made in the study of CyHV-2, the mechanism of interaction between CyHV-2 and crucian carp is still unclear.…”

Section: Introductionmentioning

confidence: 99%

Circ-Udg Derived from Cyprinid Herpesvirus 2 Promotes Viral Replication

et al. 2022

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“…Recombinant truncated membrane protein ORF25 was found to have certain immune protective effect against virus infection and was developed as a potential vaccine (Yuan et al, 2020; Zhou et al, 2015). ORF104 was found that can activate p38 mark pathway to help the virus invade cells, so it can prevent infection by blocking ORF104 and preventing p38 mark activation (Du et al, 2015). Moreover, recombinant membrane proteins of CyHV‐2 ORF25, ORF25C, ORF25D, ORF30, ORF124, ORF131, ORF136, ORF142a and ORF146 were expressed and neutralizing in vivo and the result showed that tORF25, tORF25C and tORF146 had better immunize protective effect against CyHV‐2 infection in crucian carp (Cao et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of monoclonal antibodies specific for cyprinid herpesvirus 2

Zhao

Gao

Zheng

et al. 2022

Journal of Fish Diseases

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Infections of Cyprinid herpesvirus 2 in goldfish and farmed crucian carp (Carassius auratus gibelio) are still an urgent problem worldwide. Detection and prevention are necessary for the control of haematopoietic necrosis disease caused by CyHV‐2. Although many sensitive molecular diagnostic methods have been developed, effective immunodiagnosis and neutralization approaches based on monoclonal antibodies (MAbs) against CyHV‐2 are still important to CyHV‐2 study. In this experiment, purified CyHV‐2 was used as antigens to produce monoclonal antibodies (Mabs). Six Mabs bound to different proteins were selected by Dot‐blot screening and Western‐blot analysis, and no one had cross‐reactivity with closely related koi herpesvirus. Among them, Mabs 2E1‐B10, 1F5‐A3 and 4C4‐A7 belonged to IgG1 isotype, while other three Mabs 3G9‐B11, 3B4‐G5 and 4F4‐B7 belonged to IgM isotype. These six Mabs all could specifically detect CyHV‐2 in CyHV‐2 infected caudal fin of Carassius auratus gibelio (GiCF) cells by immunofluorescence assays. Then, the neutralization ability was tested in vitro, and the result showed that all six Mabs can attenuate CPE by CyHV‐2 in vitro among which 2E1‐B10 had the best neutralization ability. The virus proteins recognized by these six Mabs were identified by mass spectrometry identification, and the result showed they probably were ORF88, ORF55R, ORF115 and ORF151R. This study is the first to prepare Mabs by purifying CyHV‐2, which will provide a practical basis for the in‐depth study of CyHV‐2 virus.

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CyHV-2 ORF104 activates the p38 MAPK pathway

Cited by 12 publications

References 36 publications

Natural Product Ginsenoside 20(S)-25-Methoxyl-Dammarane-3β, 12β, 20-Triol in Cancer Treatment: A Review of the Pharmacological Mechanisms and Pharmacokinetics

Natural Product Ginsenoside 20(S)-25-Methoxyl-Dammarane-3β, 12β, 20-Triol in Cancer Treatment: A Review of the Pharmacological Mechanisms and Pharmacokinetics

Circ-Udg Derived from Cyprinid Herpesvirus 2 Promotes Viral Replication

Development and characterization of monoclonal antibodies specific for cyprinid herpesvirus 2

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