Cyclosporine A-loaded and stem cell-seeded electrospun nanofibers for cell-based therapy and local immunosuppression

Holáň, Vladimı́r; Chudíčková, Milada; Trošan, Peter; Svobodová, Eliška; Krulová, Magdaléna; Kubinová, Šárka; Syková, Eva; Širc, Jakub; Michálek, Jiřı́; Juklickova, Martina; Munzarová, Marcela; Zajı́cová, Alena

doi:10.1016/j.jconrel.2011.07.022

Cited by 43 publications

(27 citation statements)

References 29 publications

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“…Nanofiber scaffolds were prepared from the biocompatible polymer poly(L‐lactic) acid (PLA) by a needleless electrospinning procedure, as we have described [23]. In brief, PLA polymer was dissolved in chloroform and two other solvents, 1,2‐dichlorethane and ethyl acetate, were added to this solution.…”

Section: Methodsmentioning

confidence: 99%

A Comparative Study of the Therapeutic Potential of Mesenchymal Stem Cells and Limbal Epithelial Stem Cells for Ocular Surface Reconstruction

Holáň

Trošan

Čejka

et al. 2015

Stem Cells Translational Medicine

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106

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Corneal regeneration x Stem cell-based therapy ABSTRACTStem cell-based therapy has become an attractive and promising approach for the treatment of severe injuries or thus-far incurable diseases. However, the use of stem cells is often limited by a shortage of available tissue-specific stem cells; therefore, other sources of stem cells are being investigated and tested. In this respect, mesenchymal stromal/stem cells (MSCs) have proven to be a promising stem cell type. In the present study, we prepared MSCs from bone marrow (BM-MSCs) or adipose tissue (Ad-MSCs) as well as limbal epithelial stem cells (LSCs), and their growth, differentiation, and secretory properties were compared. The cells were grown on nanofiber scaffolds and transferred onto the alkaliinjured eye in a rabbit model, and their therapeutic potential was characterized. We found that BM-MSCs and tissue-specific LSCs had similar therapeutic effects. Clinical characterization of the healing process, as well as the evaluation of corneal thickness, re-epithelialization, neovascularization, and the suppression of a local inflammatory reaction, were comparable in the BM-MSC-and LSC-treated eyes, but results were significantly better than in injured, untreated eyes or in eyes treated with a nanofiber scaffold alone or with a nanofiber scaffold seeded with Ad-MSCs. Taken together, the results show that BM-MSCs' therapeutic effect on healing of injured corneal surface is comparable to that of tissuespecific LSCs. We suggest that BM-MSCs can be used for ocular surface regeneration in cases when autologous LSCs are absent or difficult to obtain. STEM CELLS

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Section: Methodsmentioning

confidence: 99%

A Comparative Study of the Therapeutic Potential of Mesenchymal Stem Cells and Limbal Epithelial Stem Cells for Ocular Surface Reconstruction

Holáň

Trošan

Čejka

et al. 2015

Stem Cells Translational Medicine

Self Cite

106

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“…[1][2][3] Besides, inflammation is a critical component of tumor progression; the inflammatory cells are powerful tumor promoters in the early neoplastic progress. 14-17 Therapeutic molecules such as siRNA, 18,19 growth factors, 20, 21 and anti-inflammatory drugs 6,22,23 can be encapsulated into electrospun fibers directly through mixing in polymer solution for electrospinning. 5 New functional biomaterials that can regulate the inflammation are still on demand.…”

Section: Introductionmentioning

confidence: 99%

Delivery of dexamethasone from electrospun PCL–PEO binary fibers and their effects on inflammation regulation

Rubert

et al. 2015

RSC Adv.

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Electrospinning of immiscible polymer blends of PCL and PEO has rendered the solid, straight and hydrophobic PCL fibers into porous, hydrophilic microfibers. In this study, electrospun PCL, 11.4%PEO-88.6%PCL and 23.1%PEO-76.9%PCL fibers were loaded with dexamethasone (DEX) without changing the morphology. Their anti-inflammatory properties on Raw 264.7 cells were compared in vitro. All fibers were found biocompatible and the encapsulation of DEX could alleviate LPS induced inflammation response. Differences in surface topography, chemical composition, wettability and release kinetics among the different fibers collectively affected the regulation on inflammatory related gene expressions.

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“…25,26 The incorporation of various additional substances, such as pharmacologically active compounds, in the nanofibers can be accomplished by simply adding the substances to the polymer solution. In our previous work 27 using Nanospider™ technology, we demonstrated that the immunosuppressive drug cyclosporine A can be incorporated directly into electrospun nanofibers without any loss of its pharmacological activity.…”

Section: Introductionmentioning

confidence: 99%

Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses

Širc¹,

Kubinová²,

Hobzová³

et al. 2012

IJN

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Polyvinyl alcohol nanofibers incorporating the wide spectrum antibiotic gentamicin were prepared by Nanospider™ needleless technology. A polyvinyl alcohol layer, serving as a drug reservoir, was covered from both sides by polyurethane layers of various thicknesses. The multilayered structure of the nanofibers was observed using scanning electron microscopy, the porosity was characterized by mercury porosimetry, and nitrogen adsorption/desorption measurements were used to determine specific surface areas. The stability of the gentamicin released from the electrospun layers was proved by high-performance liquid chromatography (HPLC) and inhibition of bacterial growth. Drug release was investigated using in vitro experiments with HPLC/MS quantification, while the antimicrobial efficacy was evaluated on Gram-positive Staphylococcus aureus and Gram-negative Pseudomonas aeruginosa. Both experiments proved that the released gentamicin retained its activity and showed that the retention of the drug in the nanofibers was prolonged with the increasing thickness of the covering layers.

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Cyclosporine A-loaded and stem cell-seeded electrospun nanofibers for cell-based therapy and local immunosuppression

Cited by 43 publications

References 29 publications

A Comparative Study of the Therapeutic Potential of Mesenchymal Stem Cells and Limbal Epithelial Stem Cells for Ocular Surface Reconstruction

A Comparative Study of the Therapeutic Potential of Mesenchymal Stem Cells and Limbal Epithelial Stem Cells for Ocular Surface Reconstruction

Delivery of dexamethasone from electrospun PCL–PEO binary fibers and their effects on inflammation regulation

Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses

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