Cyclosporin A inhibits apolipoprotein A-I-induced early events in cellular cholesterol homeostasis in rat astrocytes

Kheirollah, Alireza; Ito, Jin‐ichi; Nagayasu, Yuko; Lu, Rui; Yokoyama, Shinji

doi:10.1016/j.neuropharm.2006.04.020

Cited by 12 publications

(8 citation statements)

References 35 publications

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“…Further, patients with kidney transplants show low HDL levels due to reduction in serum APOA1 levels [52,53]. It has been clearly demonstrated in cell lines harbouring APOA1 reporter gene that activation of the calcineurin pathway by intracellular calcium mobilization stimulates APOA1 gene expression and calcineurin inhibition by CsA results in reduced APOA1 gene expression [54].…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of zebrafish (Danio rerio) embryos exposed to cyclosporine A

Ponnudurai

Ahmad

Bhardwaj

et al. 2012

Journal of Proteomics

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of zebrafish (Danio rerio) embryos exposed to cyclosporine A

Ponnudurai

Ahmad

Bhardwaj

et al. 2012

Journal of Proteomics

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“…Proteins such as caveolin-1 and cyclophilin A are also translocated to this fraction in the cytosol of rat astrocytes after the apoA-I treatment for 90 min. A cyclophilin A-specific inhibitor, cyclosporin A, suppressed not only the translocation of newly synthesized lipid and caveolin-1 to the cytosol but also the cholesterol efflux in apoA-I-treated rat astrocytes [31, 68]. These findings suggest that the newly synthesized cholesterol and phospholipids exist as lipoprotein-like particles together with proteins such as caveolin-1 and cyclophilin A in the cytosol.…”

Section: Exogenous Apoa-i-mediated Intracellular Cholesterol Transmentioning

confidence: 99%

ApoA-I/HDL Generation and Intracellular Cholesterol Transport through Cytosolic Lipid-Protein Particles in Astrocytes

Ito

Michikawa

2014

Journal of Lipids

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Exogenous apolipoprotein A-I (apoA-I) associates with ATP-binding cassette transporter A1 (ABCA1) on the cell surface of astrocytes like various peripheral cells and enhances the translocation of newly synthesized cholesterol from the endoplasmic reticulum/Golgi apparatus (ER/Golgi) to the cytosol. The cholesterol translocated to the cytosol is incorporated to cytosolic lipid-protein particles (CLPP) together with phospholipids and proteins such as sphingomyelin, phosphatidylcholine, caveolin-1, protein kinase Cα (PK-Cα), and cyclophilin A. The CLPP are high density lipoproteins- (HDL-)like cytosolic lipid-protein complex with densities of 1.09–1.16 g/mL and diameters of 17-18 nm. The association of exogenous apoA-I with cellular ABCA1 induces tyrosine phosphorylation, activation, and translocation to the CLPP of ABCA1-associated phospholipase Cγ (PL-Cγ) in rat astrocytes. Furthermore, PK-Cα is translocated and activated to/in the CLPP through theproduction of diacylglyceride in the CLPP. ApoA-I enhances both the association of CLPP with microtubules and the phosphorylation of α-tubulin as a component of microtubules. The CLPP are dissociated from microtubules after α-tubulin in microtubules is phosphorylated by the CLPP-associated PK-Cα. The association and dissociation between CLPP and microtubules may participate in the intracellular transport of cholesterol to the plasma membrane.

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“…移行する．移行した phospholipase Cγにより CLPP では diacylglyceride 産生が亢進し，続いて protein kinase Cαが CLPP に移行する 18,19) (Fig. 6)…”

Section: はじめにunclassified

Mechanism for ApoE/HDL Generation and Cholesterol Homeostasis in the Brain

Ito

2007

membrane

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Neural cells such as neurons and glias in the central nervous system (CNS) appear in a multi-process with a large cell surface. Accordingly the CNS is a cholesterol-rich organ that accounts for approximately 25% of total body cholesterol in humans. As circulating plasma lipoproteins is segregated by the blood-brain barrier (BBB) from the CNS of vertebrates, a unique system for intercellular cholesterol transport functions in the brain. This review article describes the contribution of apoE/HDL generation by astrocytes to cholesterol homeostasis in the brain.

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Cyclosporin A inhibits apolipoprotein A-I-induced early events in cellular cholesterol homeostasis in rat astrocytes

Cited by 12 publications

References 35 publications

Proteomic analysis of zebrafish (Danio rerio) embryos exposed to cyclosporine A

Proteomic analysis of zebrafish (Danio rerio) embryos exposed to cyclosporine A

ApoA-I/HDL Generation and Intracellular Cholesterol Transport through Cytosolic Lipid-Protein Particles in Astrocytes

Mechanism for ApoE/HDL Generation and Cholesterol Homeostasis in the Brain

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