Cyclosporin A distribution in cholesterol-sphingomyelin artificial membranes modeled as Langmuir monolayers

Wnętrzak, Anita; Makyła-Juzak, Katarzyna; Chachaj‐Brekiesz, Anna; Lipiec, Ewelina; Romeu, Nuria Vila; Dynarowicz-Łątka, Patrycja

doi:10.1016/j.colsurfb.2018.03.031

Cited by 29 publications

(17 citation statements)

References 50 publications

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“…This indicates the liquid-expanded state of the Chol-CsA monolayers. Similar observations were described by Wnętrzak et al (2018) for the Chol-sphingomyelin mixed films. Moreover, CsA provokes formation of domain structure within the membrane with the coexisting LE/LC phases (see Sect.…”

Section: Compression Modulus Analysissupporting

confidence: 88%

“…The liquid condensed state of Chol proved Cs −1 high values up to about 900 mN/m (Fig. 4) and even very small additions of CsA cause a dramatic decrease of this parameter (Cs −1 = ~ 100 mN/m at π = 15 mN/m) changing the monolayer to liquid expanded/liquid-condensed state (Wnętrzak et al 2018). This behaviour was also observed by Söderlund et al (1999).…”

Section: Morphology and Thicknessmentioning

confidence: 95%

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Properties of the Langmuir and Langmuir–Blodgett monolayers of cholesterol-cyclosporine A on water and polymer support

et al. 2019

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The paper deals with the cholesterol-cyclosporine A (Chol-CsA) monolayers at the air/water interface investigated using the Langmuir trough coupled with the Brewster's angle microscopy. The compressed films were transferred onto the PEEK polymer support by means of the Langmuir-Blodgett technique. To improve molecules adhesion and organization the PEEK surface was treated with air plasma before thin films deposition. The obtained surfaces were characterized by means of atomic force microscope (AFM). Then, the wettability of the supported monolayers was determined by the contact angle measurements. Finally, the surface free energy and its components were evaluated from the theoretical approach proposed by van Oss et al. The obtained results reveal correlation between properties of the Langmuir monolayers at the air/water interface and those of the Langmuir-Blodgett films on PEEK. This was found to be helpful for understanding the wettability of organized molecular films on the polymer support as far as biocompatibility improve is concerned. The preparation of films with defined polarity and various compositions is an important step in the development of polymer surfaces with increased biofunctionality. It is believed that the results presented in this paper can be exploited in the in vivo studies.

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Section: Compression Modulus Analysissupporting

confidence: 88%

Section: Morphology and Thicknessmentioning

confidence: 95%

Properties of the Langmuir and Langmuir–Blodgett monolayers of cholesterol-cyclosporine A on water and polymer support

et al. 2019

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“…Collapse pressure values are very similar and do not differ significantly from the π c obtained for pure cholesterol monolayer (around 46 mN•m −1 ) [43]. This suggests some immiscibility phenomena [43][44][45] according with the presence of two domains. C −1 s values allow us to postulate that PEG mainly mixes with phospholipid-rich domains.…”

Section: Ocular Tolerancesupporting

confidence: 68%

Dexibuprofen Biodegradable Nanoparticles: One Step Closer towards a Better Ocular Interaction Study

et al. 2020

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Ocular inflammation is one of the most prevalent diseases in ophthalmology, which can affect various parts of the eye or the surrounding tissues. Non-steroidal anti-inflammatory drugs (NSAIDs) such as ibuprofen, are commonly used to treat ocular inflammation in the form of eye-drops. However, their bioavailability in ocular tissues is very low (less than 5%). Therefore, drug delivery systems such as biodegradable polymeric PLGA nanoparticles constitute a suitable alternative to topical eye administration, as they can improve ocular bioavailability and simultaneously reduce drug induced side effects. Moreover, their prolonged drug release can enhance patient treatment adherence as they require fewer administrations. Therefore, several formulations of PLGA based nanoparticles encapsulating dexibuprofen (active enantiomer of Ibuprofen) were prepared using the solvent displacement method employing different surfactants. The formulations have been characterized and their interactions with a customized lipid corneal membrane model were studied. Ex vivo permeation through ocular tissues and in vivo anti-inflammatory efficacy have also been studied.

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“…They were interpreted as considering that cholesterol protected the cell membrane from the effects of LBP. Another example of lipid mixtures in Langmuir monolayers included different proportions of Chol and sphingomyelin (SM) in a study of effects from the antimalarial drug cyclosporine A 69 . Cholesterol is known to affect membrane fluidity 70,71 and forms the so-called lipid rafts along with SM 72,73 .…”

Section: Summary Of Recent Resultsmentioning

confidence: 99%

“…Cholesterol is known to affect membrane fluidity 70,71 and forms the so-called lipid rafts along with SM 72,73 . Wnętrzak et al 69 observed that cyclosporine A was distributed on the monolayers in different ways, depending on the Chol-SM proportion. Cyclosporine A induces modifications in SM-Chol model membranes, especially in their mechanical properties.…”

Section: Summary Of Recent Resultsmentioning

confidence: 99%

Recent advances in the use of Langmuir monolayers as cell membrane models

Pereira

Oliveira

2021

Eclet. Quim. J.

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Understanding the role of biomolecules in cells at the molecular level has been the trade of Prof. Marcio Francisco Colombo and Prof. Jo�o Ruggiero Neto in their carriers, which is why it was found appropriate to address the use of Langmuir monolayers as cell membrane models in this special issue. In the review paper, we elaborate upon the reasons why Langmuir monolayers are good models with the possible control of membrane composition and molecular packing. After describing several experimental methods to characterize the Langmuir monolayers, we discuss selected results from the last five years where monolayers were made to interact with pharmaceutical drugs, emerging pollutants and other biologically-relevant molecules. The challenges to take the field forward are also commented upon.

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Cyclosporin A distribution in cholesterol-sphingomyelin artificial membranes modeled as Langmuir monolayers

Cited by 29 publications

References 50 publications

Properties of the Langmuir and Langmuir–Blodgett monolayers of cholesterol-cyclosporine A on water and polymer support

Properties of the Langmuir and Langmuir–Blodgett monolayers of cholesterol-cyclosporine A on water and polymer support

Dexibuprofen Biodegradable Nanoparticles: One Step Closer towards a Better Ocular Interaction Study

Recent advances in the use of Langmuir monolayers as cell membrane models

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